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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 7,696 results for: Pol

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Showing: 861 - 880 of 7696 results

  7. Polymerase expression - pCMV-Phi29 (LM2708)

    Plasmid
    #208965
    Purpose
    Unfused Phi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Publication
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Phi29-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    Phi29(-exo;D169A)
    Promoter
    CMV and T7
    Available since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208965-plasmid-map-sequence-id-415813

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  15. Ec dnaN-pHK/P

    Plasmid
    #237239
    Purpose
    over-express E. coli hk/P-dnaN (Pol III beta sliding clamp subunit, N-terminal cleveable his-kinase tag)
    Depositor
    Michael O'Donnell
    Insert
    dnaN (beta), N-terminal his-kinase cleavable tag (dnaN E. coli)
    Tags
    his-10+PKA kinase motif+PreScission Protease clea…
    Expression
    Bacterial
    Available since
    April 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    237239-plasmid-map-sequence-id-469256

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  16. Sc Pol3-FLAG(Ec)-pRSF405/GAL

    Plasmid
    #239198
    Purpose
    Overexpress Sc Pol3(C-terminal 3X FLAG tag) when integrated into yeast (S. cer)
    Depositor
    Michael O'Donnell
    Insert
    Pol3 (POL3 Budding Yeast)
    Tags
    3X FLAG
    Expression
    Yeast
    Mutation
    E. coli codon bias (synthetic gene), but same ami…
    Available since
    June 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    239198-plasmid-map-sequence-id-474564

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  18. pAAV.CMV.SV40.THBS1-HA-KDEL.SV40(polyA)

    Plasmid
    #156164
    Purpose
    Expresses murine Thrombospondin-1 (THBS1) protein with HA-tag at C-terminus and KDEL (ER retention) sequence
    Depositor
    Kevin Park
    Publication
    Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d
    Insert
    Thrombospondin-1 (Thbs1 Mouse)
    Use
    AAV
    Tags
    HA-tag
    Expression
    Mammalian
    Mutation
    THBS1 with addition of ER-retention sequence KDEL
    Promoter
    CMV
    Available since
    Aug. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    156164-plasmid-map-sequence-id-309334

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Showing: 861 - 880 of 7696 results