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pCMV-T7-AcrIIA5-NLS(SV40) (KAC203)

Plasmid

#133802

Purpose

CMV and T7 promoter expression plasmid for human codon optimized AcrIIA5 with C-terminal NLS (SV40)

Depositor

Joseph Bondy-Denomy, Benjamin Kleinstiver

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized AcrIIA5

Tags

NLS(SV40)

Expression

Mammalian

Mutation

n/a

Promoter

CMV and T7

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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E3020X

Plasmid

#21366

Depositor

Gregory Germino

Article

Qian et al Proc Natl Acad Sci U S A. 2002 Dec 24. 9

Insert

autosomal dominant polycystic kidney disease type I (PKD1 Human)

Expression

Mammalian

Mutation

Changed E3020 to stop codon

Available Since

Feb. 17, 2010

Availability

Academic Institutions and Nonprofits only
WT (AF20)

Plasmid

#21359

Depositor

Gregory Germino

Article

Qian et al Proc Natl Acad Sci U S A. 2002 Dec 24. 9

Insert

autosomal dominant polycystic kidney disease type I (PKD1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

wild-type sequence*

Available Since

Jan. 21, 2010

Availability

Academic Institutions and Nonprofits only

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WT (REP10)

Plasmid

#21368

Depositor

Gregory Germino

Article

Qian et al Proc Natl Acad Sci U S A. 2002 Dec 24. 9

Insert

autosomal dominant polycystic kidney disease type I (PKD1 Human)

Expression

Mammalian

Available Since

Jan. 4, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3_attR1-ORF-attR2-NTEV-TCS-GV-2xHA_DEST

Plasmid

#194385

Purpose

Gateway Destination vector for split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

attR1-ORF-attR2-NTEV-TCS-GV-2xHA

Tags

2xHA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pPpT4- HTA1prom-TIR1 F74G -FLAG

Plasmid

#189728

Purpose

Enables expression of OsTIR1(F74G) from the HTA1 promoter, used to implement the AID2 system in Komagataella phaffii (original name pLB220)

Depositor

Anita Emmerstorfer-Augustin

Article

Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

Insert

OsTIR1

Tags

FLAG

Expression

Yeast

Promoter

HTA1

Available Since

Sept. 20, 2022

Availability

Academic Institutions and Nonprofits only

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psbA2(noATbox)-PHLS (c)

Plasmid

#52308

Purpose

Replaces the psbA2 gene in Synechocystis with the PHLS gene

Depositor

Anastasios Melis

Article

Formighieri et al Planta. 2014 Aug;240(2):309-24. doi: 10.

Inserts

beta-phellandrene synthase
Chloramphenicol resistance

Use

Synthetic Biology

Expression

Bacterial

Mutation

CAAATACA box in the psbA2 promoter to ggcgcgcc

Promoter

psbA2

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

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psbA2-Ptrc-T7-g10-PHLS (e)

Plasmid

#52310

Purpose

Replaces the psbA2 gene in Synechocystis with the Ptrc promoter-T7-g10-PHLS gene

Depositor

Anastasios Melis

Article

Formighieri et al Planta. 2014 Aug;240(2):309-24. doi: 10.

Inserts

beta-phellandrene synthase
Chloramphenicol resistance

Use

Synthetic Biology

Expression

Bacterial

Promoter

Ptrc-T7

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

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psbA2-PHLS (b)

Plasmid

#52307

Purpose

Replaces the psbA2 gene in Synechocystis with the PHLS gene

Depositor

Anastasios Melis

Article

Formighieri et al Planta. 2014 Aug;240(2):309-24. doi: 10.

Inserts

beta-phellandrene synthase
Chloramphenicol resistance

Use

Synthetic Biology

Expression

Bacterial

Promoter

psbA2

Available Since

May 21, 2014

Availability

Academic Institutions and Nonprofits only

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psbA2-Ptrc-PHLS (d)

Plasmid

#52309

Purpose

Replaces the psbA2 gene in Synechocystis with the Ptrc promoter - PHLS gene

Depositor

Anastasios Melis

Article

Formighieri et al Planta. 2014 Aug;240(2):309-24. doi: 10.

Inserts

beta-phellandrene synthase
Chloramphenicol resistance

Use

Synthetic Biology

Expression

Bacterial

Promoter

Ptrc

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

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pCK761

Plasmid

#242916

Purpose

Constitutive expression of dCas9, αNTD-MCP, and PspF-λN22

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

Insert

PspF-λN22 (pspF Synthetic)

Use

CRISPR and Synthetic Biology

Promoter

BBa_J23107

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCK948

Plasmid

#242917

Purpose

Constitutive expression of dCas9, MCP-TetD, and PspF-λN22

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

Insert

PspF-λN22 (pspF Synthetic)

Use

CRISPR and Synthetic Biology

Promoter

BBa_J23107

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pKO025

Plasmid

#242913

Purpose

aTc-inducible expression of Spy-dCas9-AsiA and Sth1-dCas9, and constitutive expression of MCP-SoxS

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

Insert

Sth1-dCas9

Use

CRISPR and Synthetic Biology

Promoter

Ptet

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCK660

Plasmid

#242914

Purpose

Constitutive expression of dCas9, MCP-SoxS, and PspF-λN22

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

Insert

PspF-λN22 (pspF Synthetic)

Use

CRISPR and Synthetic Biology

Promoter

BBa_J23107

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pWR58

Plasmid

#202795

Purpose

integrating bioluminescene reporter for Cas9 activity in S. stipitis

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

P-TDH3/coCBG(first portion)-YUM1
P-TEF1/ShBle/T-ACT1
YUM1-coCBG(last portion)/T-ADH2
URA3 and flanking sequence

Expression

Yeast

Available Since

Dec. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3_ReBphP-PCM_IRES_sfGFP

Plasmid

#166917

Purpose

Express ReBphP-PCM and sfGFP

Depositor

Andre Stiel

Article

Mishra et al Sci Adv. 2020 Jun 12;6(24):eaaz6293. doi

Inserts

ReBphP-PCM
sfGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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p3.2mar(T->G)-Luc

Plasmid

#63697

Purpose

3.2 kb marine armor plate enhancer with a single T->G bp change driving Luciferase expression

Depositor

David Kingsley

Article

O'Brown et al Elife. 2015 Jan 28;4:e05290. doi: 10.755

Insert

3.2 kb marine stickleback armor plate enhancer with a single T->G base pair change

Tags

Luciferase

Expression

Mammalian

Promoter

TATA box

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

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p3.2mar(T->G)-GFP

Plasmid

#63695

Purpose

3.2 kb marine armor plate enhancer with a single T->G bp change driving GFP reporter

Depositor

David Kingsley

Article

O'Brown et al Elife. 2015 Jan 28;4:e05290. doi: 10.755

Insert

3.2 kb marine stickleback armor plate enhancer with a single T->G base pair change

Tags

EGFP

Expression

Mammalian

Mutation

T->G bp change

Promoter

hsp70

Available Since

April 1, 2015

Availability

Academic Institutions and Nonprofits only

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pN3-MGA

Plasmid

#107715

Purpose

Expression plasmid for 3xFLAG-tagged MGA

Depositor

Guntram Suske

Article

Stielow et al PLoS Genet. 2018 Jan 30;14(1):e1007193.

Insert

MGA (MGA Human)

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV-T7-AcrIIC3-NLS(SV40) (KAC209)

Plasmid

#134341

Purpose

CMV and T7 promoter expression plasmid for human codon optimized AcrIIC3 with C-terminal NLS (SV40)

Depositor

Joseph Bondy-Denomy, Benjamin Kleinstiver

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized AcrIIC3

Tags

NLS(SV40)

Expression

Mammalian

Promoter

CMV and T7

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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