We narrowed to 5,835 results for: pCas

1. pCas-4/1Δ2_VB

   Plasmid

   #186443

   Purpose

   Encodes Cas4/1, leader, repeat and one spacer from A. acidoterrestris (type V-B)

   Depositor

   John van der Oost

   Article

   Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

   Insert

   Cas4/1
   

   Use

   CRISPR

   Promoter

   T7

   Available Since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCas_4(I-U)/1_2_VB

   Plasmid

   #186445

   Purpose

   Encodes Cas4/1, Cas2, leader, repeat and one spacer from A. acidoterrestris (type V-B). Cas4 domain swapped with type U-I Cas4 domain from G. sulfurreducens

   Depositor

   John van der Oost

   Article

   Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

   Insert

   Cas4/1 and Cas2
   

   Use

   CRISPR

   Mutation

   Cas4 domain swapped with Cas4 domain from G. sulf…

   Promoter

   T7

   Available Since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. 2X_pX458_pSpCas9(BB)-2A-GFP_eSOX17.2_KO

   Plasmid

   #195495

   Purpose

   Cas9-2A-GFP expression vector bearing two sgRNAs targeting the core endoderm distal enhancer of human SOX17

   Depositor

   Alexander Meissner

   Article

   Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

   Insert

   sgRNA (SOX17 Human)
   

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. eSpCas9-EF-5Flnc

   Plasmid

   #174870

   Purpose

   CRISPR vector for generating Flnc STREAMING-tag KI cell

   Depositor

   Hiroshi Ochiai

   Article

   Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

   Insert

   sgRNA for mouse Flnc (Flnc Synthetic)
   

   Use

   CRISPR

   Available Since

   Dec. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. eSpCas9-EF-5Usp5

   Plasmid

   #174873

   Purpose

   CRISPR vector for generating Usp5 STREAMING-tag KI cell

   Depositor

   Hiroshi Ochiai

   Article

   Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

   Insert

   sgRNA for mouse Usp5 (Usp5 Synthetic)
   

   Use

   CRISPR

   Available Since

   Dec. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. eSpCas9-EF-5Wnk1

   Plasmid

   #174874

   Purpose

   CRISPR vector for generating Wnk1 STREAMING-tag KI cell

   Depositor

   Hiroshi Ochiai

   Article

   Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

   Insert

   sgRNA for mouse Wnk1 (Wnk1 Synthetic)
   

   Use

   CRISPR

   Available Since

   Dec. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCASCADE-xylA

   Plasmid

   #158611

   Purpose

   Low phosphate inducible gRNA silencing the xylA promoter

   Depositor

   Michael Lynch

   Article

   Li et al Metab Eng. 2021 Jan 15. pii: S1096-7176(

   Insert

   xylA guide array
   

   Expression

   Bacterial

   Promoter

   E . coli ugpB gene promoter, low phosphate induct…

   Available Since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-hPPP3R2

   Plasmid

   #179163

   Purpose

   Expression vector of human protein phosphatase 3, regulatory subunit B, beta isoform (PPP3R2), CAG promoter, rabbit globin poly(A) signal.

   Depositor

   Masahito Ikawa

   Article

   Miyata et al Science. 2015 Oct 23;350(6259):442-5. do

   Insert

   protein phosphatase 3, regulatory subunit B, beta isoform (PPP3R2 Human)
   

   Expression

   Mammalian

   Available Since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-mPpp3r2

   Plasmid

   #179161

   Purpose

   Expression vector of mouse protein phosphatase 3, regulatory subunit B, beta isoform (Ppp3r2), CAG promoter, rabbit globin poly(A) signal.

   Depositor

   Masahito Ikawa

   Article

   Miyata et al Science. 2015 Oct 23;350(6259):442-5. do

   Insert

   protein phosphatase 3, regulatory subunit B, beta isoform (Ppp3r2 Mouse)
   

   Expression

   Mammalian

   Available Since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-mSpata33-PA

    Plasmid

    #179129

    Purpose

    Expression vector of mouse spermatogenesis associated 33 (Spata33) tagged with PA at C-terminus, CAG promoter, rabbit globin poly(A) signal.

    Depositor

    Masahito Ikawa

    Article

    Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

    Insert

    spermatogenesis associated 33 (Spata33 Mouse)
    

    Tags

    PA

    Expression

    Mammalian

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-mDrc3-PA

    Plasmid

    #176467

    Purpose

    Expression vector of mouse dynein regulatory complex subunit 3 (Drc3) tagged with PA at C-terminus.

    Depositor

    Masahito Ikawa

    Article

    Morohoshi et al PLoS Genet. 2020 Jan 21;16(1):e1008585.

    Insert

    dynein regulatory complex subunit 3 (Drc3 Mouse)
    

    Tags

    PA

    Expression

    Mammalian

    Available Since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAFNF-Rhoa

    Plasmid

    #163605

    Purpose

    Expresses RhoA Flp-dependently in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Rhoa (Rhoa Mouse)
    

    Expression

    Mammalian

    Available Since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAFNF-EGFP

    Plasmid

    #163609

    Purpose

    Expresses EGFP Flp-dependently in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Egfp
    

    Expression

    Mammalian

    Available Since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAFNF-Cdc42

    Plasmid

    #163606

    Purpose

    Expresses Cdc42 Flp-dependently in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Cdc42 (Cdc42 Mouse)
    

    Expression

    Mammalian

    Available Since

    Aug. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-mEGFP-HO-G

    Plasmid

    #164163

    Purpose

    Expresses mEGFP (monomeric EGFP with the A206K mutation) under the control of CAG promoter, codon-optimized for Homo sapiens, basic vector, multiclonig site at C terminal

    Depositor

    Isei Tanida

    Article

    Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1

    Insert

    mEGFP A236K
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAMTA2.1.0-gDNA

    Plasmid

    #132456

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    CAMTA2 (CAMTA2 Human)
    

    Use

    CRISPR

    Available Since

    July 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCas9n-Nanog-L

    Plasmid

    #122302

    Purpose

    Expresses sgRNA targeting mouse Nanog and Cas9 nickase in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    sgRNA for mouse Nanog (Nanog Synthetic)
    

    Expression

    Mammalian

    Available Since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCas9n-Nanog-R

    Plasmid

    #122303

    Purpose

    Expresses sgRNA targeting mouse Nanog and Cas9 nickase in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    sgRNA for mouse Nanog (Nanog Synthetic, Mouse)
    

    Expression

    Mammalian

    Available Since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAGGS-synPCB2.2

    Plasmid

    #139479

    Purpose

    Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.

    Depositor

    Kazuhiro Aoki

    Article

    Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

    Insert

    MTS-Fnr-Fd-P2A-MTS-HO1-PcyA-FLAG
    

    Expression

    Mammalian

    Mutation

    V554L

    Promoter

    CAG promoter

    Available Since

    April 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-T3-HypaCAS9-pA

    Plasmid

    #131467

    Purpose

    Expresses HypaCas9 nuclease in mammalian cells. Used to modify genome of mouse embroys

    Depositor

    Wataru Fujii

    Article

    Ikeda et al Commun Biol. 2019 Oct 10;2:371. doi: 10.

    Insert

    Codon optimized HypaCas9
    

    Use

    CRISPR

    Tags

    Flag, NLS, and polyA

    Expression

    Mammalian

    Available Since

    Nov. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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