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We narrowed to 14,953 results for: pET

Showing: 8781 - 8800 of 14953 results

  2. pX330_Actb sgRNA / hSpCas9

    Plasmid
    #172832
    Purpose
    Mammalian expression of a sgRNA targeting the intron 1of Actb (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    sgRNA targeting intron 1 of Actb under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172832-plasmid-map-sequence-id-340914

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  11. pX330_ACTB-i4 sgRNA / hSpCas9

    Plasmid
    #172828
    Purpose
    Mammalian expression of a sgRNA targeting the intron 1 position 4 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    sgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    172828-plasmid-map-sequence-id-341092

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  12. pCX-SponGee-Kras

    Plasmid
    #134777
    Purpose
    mammalian expression of SponGee-Kras, a plasma membrane-targeted but lipid raft-excluded variant of the cGMP buffer SponGee that prevents downstream signaling
    Depositor
    Xavier Nicol
    Article
    Ros et al Cell Rep. 2019 Jun 25;27(13):4003-4012.e
    Insert
    SponGee-Kras
    Use
    Tags
    FLAG and mRFP
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Jan. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    134777-plasmid-map-sequence-id-266270

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  15. pOPS0378

    Plasmid
    #133229
    Purpose
    Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.
    Depositor
    Philip Poole
    Article
    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117
    Insert
    constructed with constitutive promoter pNeo (pOGG001), mCherry (EC15071) and T-pharma (pOGG003)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    133229-plasmid-map-sequence-id-276542

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  16. pAM4951 (pCV0063)

    Plasmid
    #120084
    Purpose
    Cyanobacteria cloning vector. Chromosomal integration into Synechococcus elongatus neutral site 1 (NS1).
    Depositor
    Susan Golden
    Article
    Taton et al Nucleic Acids Res. 2014 Jul 29. pii: gku
    Insert
    Plasmid assembled from 3 CYANO-VECTOR modular devices: CV-S7942NS1-RK2BOM, CV-aadA_nt, CV-ccdB-SwaI.
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120084-plasmid-map-sequence-id-234399

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  18. VVT1

    Plasmid
    #65779
    Purpose
    The Joung Lab recommends using BPK2660 (Addgene plasmid 70709) instead of VVT1 as guide RNAs cloned into BPK2660 are more effective than this plasmid. Human expression plasmid for S. aureus Cas9 sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    SaCas9 gRNA backbone, without spacer sequence
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    65779-plasmid-map-sequence-id-112660

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  19. pHS1477

    Plasmid
    #205970
    Purpose
    MWEE01000013 Cas5-HNH operon (E. coli codon optimized) with His14-tagged Cse2 in pET45b(+)
    Depositor
    Feng Zhang
    Article
    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.
    Insert
    Cas5-HNH all proteins, with His-tagged Cas11
    Use
    Tags
    14xHis on Cas11
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    205970-plasmid-map-sequence-id-416229

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Showing: 8781 - 8800 of 14953 results