We narrowed to 16,971 results for: pET

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p141 pETF-GST RanBP10 G308R

Plasmid

#11094

Depositor

Ramesh Shivdasani

Insert

RanBP10 (Ranbp10 Mouse)

Tags

GST

Expression

Bacterial

Mutation

G308R

Available since

Dec. 16, 2005

Availability

Academic Institutions and Nonprofits only

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Kv7.4 BD (522-645, C643A)/pET28HMT

Plasmid

#111073

Purpose

Protein expression in E.Coli

Depositor

Dan Minor

Publication

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)

Tags

His Tag and MBP tag

Expression

Bacterial

Mutation

Kv7.4 BD (522-645, C643A)

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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Pet28a His6-HSPB7 (zebrafish) C117S

Plasmid

#110072

Purpose

Express C117S mutant of Zebrafish His6-HSPB7 in bacteria

Depositor

Yimon Aye

Publication

Surya et al ACS Chem Biol. 2018 Feb 8. doi: 10.1021/

Insert

His6-HSPB7 (hspb7 Zebrafish)

Tags

His6

Expression

Bacterial

Mutation

Cysteine 117 to Serine

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pET28a-MGGG-ybbr-HIS-SdrG_B1-DK

Plasmid

#117981

Purpose

SdrG B1 domain with N-terminal ybbr and sortase tags for covalent immobilization and C-terminal DK peptide for thethering with ClfB

Depositor

Hermann Gaub

Publication

Milles et al Nat Commun. 2018 Nov 12;9(1):4764. doi:

Insert

SdrG_B1

Tags

HIS, sortase, and ybbr

Expression

Bacterial

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pET28a-MGGG-ybbr-HIS-SdrG_B2-DK

Plasmid

#117982

Purpose

SdrG B2 domain with N-terminal ybbr and sortase tags for covalent immobilization and C-terminal DK peptide for thethering with ClfB

Depositor

Hermann Gaub

Publication

Milles et al Nat Commun. 2018 Nov 12;9(1):4764. doi:

Insert

SdrG_B2

Tags

HIS, sortase, and ybbr

Expression

Bacterial

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pET28a-MGGG-ybbr-HIS-SdrD_B1-DK

Plasmid

#117983

Purpose

SdrD B1 domain with N-terminal ybbr and sortase tags for covalent immobilization and C-terminal DK peptide for thethering with ClfB

Depositor

Hermann Gaub

Publication

Milles et al Nat Commun. 2018 Nov 12;9(1):4764. doi:

Insert

SdrG_B2

Tags

HIS, sortase, and ybbr

Expression

Bacterial

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pENTR2b ITGA2-tail-YPet (GFFKR/GAAKR)

Plasmid

#145144

Purpose

Shuttle vector with YPet-tagged ITGA2 cytoplasmic tail as an insert with a GFFKR/GAAKR mutation

Depositor

Johanna Ivaska

Publication

Miihkinen et al Cell Rep. 2021 Sep 14;36(11):109716. doi

Insert

ITGA2-tail-YPet (GFFKR/GAAKR) (ITGA2 Human)

Use

Gateway Cloning

Available since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a-Fgß-FIVAR-ELP-HIS-ybbR

Plasmid

#169136

Purpose

E. coli expression of FIVAR domain containing Fgß tag, ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.

Depositor

Michael Nash

Publication

Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

Insert

FIVAR (found in various architectures)

Tags

3xELP linker, 6x Histag, Fgβ peptide, and ybbr ta…

Expression

Bacterial

Available since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pETM-60-NcQDE-2_506-938-del787-838GSG_K

Plasmid

#146730

Purpose

Bacterial Expression of NcQde-2_506-938-del787-838GSG

Depositor

Elisa Izaurralde

Insert

NcQde-2_506-938-del787-838GSG

Expression

Bacterial

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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human KANK2-A670V Ankyrin Repeats pET151

Plasmid

#164055

Purpose

Expresses human KANK2 Ank domain - A670V mutant in bacterial cells

Depositor

Ben Goult

Publication

Khan et al bioRxiv 2026.06.09.731086

Insert

Kidney Ankyrin Repeat-Containing Protein 2 A670V mutant (KANK2 Human)

Tags

His-tag, TEV cleavage site

Expression

Bacterial

Promoter

T7

Available since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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mouse KANK4-Y801H Ankyrin Repeats pET151

Plasmid

#164058

Purpose

Expresses mouse KANK4 Ank domain -Y801H mutant in bacterial cells

Depositor

Ben Goult

Publication

Khan et al bioRxiv 2026.06.09.731086

Insert

Kidney Ankyrin Repeat-Containing Protein 4 Y801H mutant (Kank4 Mouse)

Tags

His-tag, TEV cleavage site

Expression

Bacterial

Promoter

T7

Available since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pTS1024-Tier1-PRPBSA-YPet-p2A-PuroR

Plasmid

#169499

Purpose

Tier-1 vector encoding PRPBSA-driven YPet in combination with a marker for puromycine selection (PRPBSA-YPet-p2A-PuroR-pA)

Depositor

Martin Fussenegger

Publication

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PRPBSA-driven YPet-P2aA-PuroR

Expression

Mammalian

Promoter

PRBSA

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pTS1047-Tier1-PhCMV-YPet-p2A-PuroR

Plasmid

#169498

Purpose

Tier-1 vector encoding PhCMV-driven YPet in combination with a marker for puromycin selection (PhCMV-YPet-p2A-PuroR-pA).

Depositor

Martin Fussenegger

Publication

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven YPet-P2aA-PuroR

Expression

Mammalian

Promoter

PhCMV

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pET28-His10-BTLAicd (AA190-289)-TwinStrep

Plasmid

#180805

Purpose

Expression of 10*His tagged human BTLA intracellular domain (AA190-289)-TwinStrep

Depositor

Enfu Hui

Publication

Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

BTLAint (AA190-289)-TwinStrep (BTLA Human)

Expression

Bacterial

Promoter

T7

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pET29b-AviTag-mEGFP-dspB(E184Q)-6xHis

Plasmid

#176573

Purpose

Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein mEGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.

Depositor

Mark Nitz

Publication

Eddenden et al Methods Enzymol. 2022;665:209-231. doi:

Insert

Dispersin B

Tags

Avitag, Hexahistidine tag, and mEGFP

Expression

Bacterial

Mutation

E184Q

Promoter

T7

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pET29b-mVenus-dspB(E184Q W330Y)-6xHis

Plasmid

#176577

Purpose

Plasmid for overexpression of recombinant His-tagged fusion protein mVenus-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.

Depositor

Mark Nitz

Publication

Eddenden et al Methods Enzymol. 2022;665:209-231. doi:

Insert

Dispersin B

Tags

Hexahistidine tag and mVenus

Expression

Bacterial

Mutation

E184Q W330Y

Promoter

T7

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-anticalin-FLN-ELP-His-ybbr

Plasmid

#168039

Purpose

Expresses anticalin targeting CTLA-4 with FLN fingerprint domain, ELP linker, 6x-His tag and ybbr tag at the C terminus

Depositor

Michael Nash

Publication

Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

Insert

Anticalin

Tags

3xELP linker, 6x Histag, ddFLN4 (C18S), and ybbr …

Expression

Bacterial

Promoter

T7 promoter

Available since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-SdrG-FLN-ELP-His-ybbr

Plasmid

#168047

Purpose

Expresses SdrG with FLN fingerprint domain, ELP linker, 6x-His tag and ybbr tag at the C terminus

Depositor

Michael Nash

Publication

Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

Insert

Staphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G

Tags

3xELP linker, 6x Histag, ddFLN4, and ybbr tag

Expression

Bacterial

Promoter

T7 promoter

Available since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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CPH3506 (pET151_Spt6 1247-1451 K1355A,K1435A)

Plasmid

#91818

Purpose

Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with K1435A and K1355A mutations

Depositor

Chris Hill

Publication

Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

Insert

SPT6 (SPT6 Budding Yeast)

Tags

6xHis

Expression

Bacterial

Mutation

changed lysine 1355 and lysine 1435 to alanines; …

Promoter

T7

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-Synapsin-YPET-3XNLS-optoNES-WPRE

Plasmid

#159947

Purpose

optical regulation of nuclear export

Depositor

Michael Courtney

Publication

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

YPET-3XNLS-optoNES

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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