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pMXBK_pFa

Plasmid

#114255

Purpose

POC12397, MetClo assembly vector with F replication origin and kanamycin resistance for BsaI-based MetClo, adaptor sequence type p-a

Depositor

Christopher A. O'Callaghan

Article

Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

Insert

LacZalpha

Use

Synthetic Biology

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pMXBK_aFb

Plasmid

#114256

Purpose

POC12398, MetClo assembly vector with F replication origin and kanamycin resistance for BsaI-based MetClo, adaptor sequence type a-b

Depositor

Christopher A. O'Callaghan

Article

Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

Insert

LacZalpha

Use

Synthetic Biology

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pMXBK_bFc

Plasmid

#114257

Purpose

POC12399, MetClo assembly vector with F replication origin and kanamycin resistance for BsaI-based MetClo, adaptor sequence type b-c

Depositor

Christopher A. O'Callaghan

Article

Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

Insert

LacZalpha

Use

Synthetic Biology

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pMXBK_aFq

Plasmid

#114258

Purpose

POC12400, MetClo assembly vector with F replication origin and kanamycin resistance for BsaI-based MetClo, adaptor sequence type a-q

Depositor

Christopher A. O'Callaghan

Article

Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

Insert

LacZalpha

Use

Synthetic Biology

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pMXBK_bFq

Plasmid

#114259

Purpose

POC12401, MetClo assembly vector with F replication origin and kanamycin resistance for BsaI-based MetClo, adaptor sequence type b-q

Depositor

Christopher A. O'Callaghan

Article

Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

Insert

LacZalpha

Use

Synthetic Biology

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pMXBK_cFq

Plasmid

#114260

Purpose

POC12402, MetClo assembly vector with F replication origin and kanamycin resistance for BsaI-based MetClo, adaptor sequence type c-q

Depositor

Christopher A. O'Callaghan

Article

Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

Insert

LacZalpha

Use

Synthetic Biology

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pMXLK_bFc

Plasmid

#114231

Purpose

POC12369, MetClo assembly vector with p15A replication origin and kanamycin resistance for BsaI-based MetClo, adaptor sequence type b-c

Depositor

Christopher A. O'Callaghan

Article

Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

Insert

LacZalpha

Use

Synthetic Biology

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pMXLK_cFd

Plasmid

#114232

Purpose

POC12370, MetClo assembly vector with p15A replication origin and kanamycin resistance for BsaI-based MetClo, adaptor sequence type c-d

Depositor

Christopher A. O'Callaghan

Article

Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

Insert

LacZalpha

Use

Synthetic Biology

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pMXLK_dFe

Plasmid

#114233

Purpose

POC12371, MetClo assembly vector with p15A replication origin and kanamycin resistance for BsaI-based MetClo, adaptor sequence type d-e

Depositor

Christopher A. O'Callaghan

Article

Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

Insert

LacZalpha

Use

Synthetic Biology

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pMXLK_aFq

Plasmid

#114234

Purpose

POC12374, MetClo assembly vector with p15A replication origin and kanamycin resistance for BsaI-based MetClo, adaptor sequence type a-q

Depositor

Christopher A. O'Callaghan

Article

Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

Insert

LacZalpha

Use

Synthetic Biology

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pMXLK_bFq

Plasmid

#114235

Purpose

POC12375, MetClo assembly vector with p15A replication origin and kanamycin resistance for BsaI-based MetClo, adaptor sequence type b-q

Depositor

Christopher A. O'Callaghan

Article

Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

Insert

LacZalpha

Use

Synthetic Biology

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pMXLK_cFq

Plasmid

#114236

Purpose

POC12376, MetClo assembly vector with p15A replication origin and kanamycin resistance for BsaI-based MetClo, adaptor sequence type c-q

Depositor

Christopher A. O'Callaghan

Article

Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

Insert

LacZalpha

Use

Synthetic Biology

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pMXLK_eFq

Plasmid

#114238

Purpose

POC12378, MetClo assembly vector with p15A replication origin and kanamycin resistance for BsaI-based MetClo, adaptor sequence type e-q

Depositor

Christopher A. O'Callaghan

Article

Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

Insert

LacZalpha

Use

Synthetic Biology

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pTK643

Plasmid

#114703

Purpose

Express NuMA(214-705)-RFP-Nano from Rosa 26 locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

NuMA(214-705)-RFP-Nano

Available Since

Oct. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pSEVA12TEC-B

Plasmid

#58307

Purpose

GeneGuard genomic cassette: for constructing ΔthyA::RBS B repA, Epsilon, FRT-kanR-FRT (low-copy support for ColE2 COR). Will support pSEVAT7Z.

Depositor

Tom Ellis

Article

Wright et al ACS Synth Biol. 2014 May 13.

Insert

Genomic cassette

Use

Synthetic Biology

Expression

Bacterial

Available Since

March 6, 2015

Availability

Academic Institutions and Nonprofits only

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pCHAC-mt-mKeima

Plasmid

#72342

Purpose

retrovirus construct for stable expressing mt-mKeima

Depositor

Richard Youle

Article

Lazarou et al Nature. 2015 Aug 20;524(7565):309-14. do

Insert

mt-mKeima

Use

Retroviral

Available Since

Jan. 8, 2016

Availability

Academic Institutions and Nonprofits only

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sgOpti

Plasmid

#85681

Purpose

Lentiviral vector to express sgRNAs. The cloning site is BsmBI.

Depositor

Eric Lander, David Sabatini

Article

Fulco et al Science. 2016 Sep 29. pii: aag2445.

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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RP4-11

Plasmid

#79812

Purpose

Modified RP4 plasmid with Km resistance and sacB gene. Easily curable helper plasmid

Depositor

Michael Hynes

Article

Quandt et al Plasmid. 2004 Jul;52(1):1-12.

Insert

Kanamycin cassette and SacB gene (sacB )

Use

Helper plasmid for mobilization

Promoter

Km

Available Since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only
Lenti_gRNA-Puro

Plasmid

#84752

Purpose

Expresses Cpf1 customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter

Depositor

Hyongbum Kim

Article

Kim et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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sc AAV miR26a eGFP

Plasmid

#21894

Depositor

Joshua Mendell

Article

Kota et al Cell. 2009 Jun 12. 137(6):1005-17.

Insert

EF1 alpha -miR26a GFP p(A)

Use

AAV; Sc

Tags

GFP

Expression

Mammalian

Mutation

N/A

Available Since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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