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pYPQ132A

Plasmid

#69274

Purpose

Golden Gate entry vector; 2nd gRNA under AtU6 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pYPQ133A

Plasmid

#69275

Purpose

Golden Gate entry vector; 3rd gRNA under AtU6 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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PX851

Plasmid

#62883

Purpose

N-term SpCas9 piece of inducible split-4 (Cas9(N)-FRB-NES split-4).

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

Insert

SpCas9 (aa 2-535)

Use

CRISPR

Tags

FRB and NES PTK2

Expression

Mammalian

Promoter

CBh

Available Since

March 4, 2015

Availability

Academic Institutions and Nonprofits only

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pSADdeltaG-DsRed2

Plasmid

#32638

Depositor

Edward Callaway

Article

Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

Insert

DsRed2

Use

Rabies virus

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 26, 2011

Availability

Academic Institutions and Nonprofits only

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pCWU6-xylR-E-luciferase

Plasmid

#190721

Purpose

fine tune expression of luciferase under the control of xylose- and theophylline- inducible system

Depositor

Chenggang Wu

Article

Bibek et al bioRxiv. 2023 Apr 24:2023.04.24.538132.

Insert

xylR-E-luciferase

Use

Luciferase

Expression

Bacterial

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5 HA GST rap2a

Plasmid

#14952

Depositor

David Sabatini

Article

Sancak et al Mol Cell. 2007 Mar 23. 25(6):903-15.

Insert

rap2a (RAP2A Human)

Tags

HA-GST

Expression

Mammalian

Available Since

May 10, 2007

Availability

Academic Institutions and Nonprofits only
pHPRT-DRGFP

Plasmid

#26476

Purpose

DR-GFP substrate (Addgene 26475) flanked by mouse hprt targeting sequences

Depositor

Maria Jasin

Article

Pierce et al Genes Dev. 2001 Dec 15. 15(24):3237-42.

Insert

pHPRT-DRGFP

Use

Mouse Targeting

Expression

Mammalian

Available Since

Nov. 3, 2010

Availability

Academic Institutions and Nonprofits only

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pWJ 1781 URA

Plasmid

#245252

Purpose

Protein expression in yeast

Depositor

Neel Shah

Article

Jiang et al Nat Commun. 2025 Jul 1;16(1):5464. doi:

Type

Empty backbone

Expression

Yeast

Available Since

Sept. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-Myr_mCherry-2A-eGFP

Plasmid

#194877

Purpose

Ratiometric Myr/Palm_mCherry control probe

Depositor

Peter Scheiffele

Article

Czernecki et al bioRxiv 2022.11.16.516849

Insert

Myristoylated/Palmitoylated_mCherry-T2A-GFP

Use

AAV

Expression

Mammalian

Promoter

human Synapsin

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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Myo1E-pmAppleC1

Plasmid

#27698

Depositor

Christien Merrifield

Article

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

Myo1E (Myo1e Mouse)

Tags

mApple

Expression

Mammalian

Available Since

May 4, 2011

Availability

Academic Institutions and Nonprofits only
pKL05

Plasmid

#104429

Purpose

super Ecliptic pHluorin in yeast expression vector

Depositor

Joel Kralj

Article

Dodd et al Sci Rep. 2017 Oct 24;7(1):13922. doi: 10

Insert

Super Ecliptic pHluorin

Expression

Yeast

Promoter

Tef1

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pMF230

Plasmid

#62546

Purpose

Broad host range plasmid for constitutive expression of the eGFP. Developed for imaging Pseudomonas aeruginosa in biofilms.

Depositor

Michael Franklin

Article

Nivens et al J Bacteriol. 2001 Feb;183(3):1047-57.

Insert

GFP mut2

Use

Broad host range plasmid

Promoter

trc

Available Since

April 21, 2015

Availability

Academic Institutions and Nonprofits only

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pYPQ131C

Plasmid

#69284

Purpose

Golden Gate entry vector; 1st gRNA under OsU6 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pYPQ132C

Plasmid

#69285

Purpose

Golden Gate entry vector; 1st gRNA under OsU6 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pGP8A-ccdB

Plasmid

#83883

Purpose

Destination vector for integration at HIS3, expression from the ADH1 promoter

Depositor

Jennifer Nemhauser

Article

Pierre-Jerome et al Proc Natl Acad Sci U S A. 2014 Jul 1;111

Type

Empty backbone

Use

Gateway destination vector

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pBAD-I-SceI

Plasmid

#60960

Purpose

Plasmid that allows an arabinose-inducible expression of the I-SceI homing endonuclease - for the en passant (two-step Red-mediated) mutagenesis

Depositor

Nikolaus Osterrieder

Article

Tischer et al Biotechniques. 2006 Feb;40(2):191-7.

Insert

I-SceI restriction enzyme

Available Since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pGL3 RARE-d2EGFP

Plasmid

#183055

Purpose

Destabilized EGFP fluorescent reporter for Retinoic Acid activity

Depositor

Chaya Kalcheim

Article

Rekler et al Elife. 2022 Apr 8;11. pii: 72723. doi: 1

Insert

d2EGFP

Promoter

RARE, SV40

Available Since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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pJYDNp

Plasmid

#162451

Purpose

Positive control plasmid carrying the eUnaG2 bilirubin dependent fluorescent protein fused to the C-terminus of Aga2p protein. This plasmid serves as an expression control.

Depositor

Gideon Schreiber

Article

Zahradník et al bioRxiv Preprint 2020

Insert

Aga2p-eUnaG2

Use

Shuttle vector bacteria/yeast

Tags

HA and myc

Promoter

GAL1

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-(empty)-TAG

Plasmid

#138209

Purpose

To facilitate restriction cloning of an RNA of interest with a TGT recognition element in the 3' region

Depositor

Neal Devaraj

Article

Busby et al Methods Enzymol. 2020;641:373-399. doi:

Type

Empty backbone

Use

T7 transcription

Tags

TAG sequence

Expression

Mammalian

Promoter

CMV Promoter, T7 Promoter

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pMyNT-kan

Plasmid

#84693

Purpose

Protein expression in mycobacteria

Depositor

Matthias Wilmanns

Article

Beckham et al Protein Sci. 2020 Oct 2. doi: 10.1002/pr

Type

Empty backbone

Tags

His6

Expression

Bacterial

Promoter

Acetamidase

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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