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pLV-hPGK-DEST-IRES-Hygro

Plasmid

#253771

Purpose

Third generation lentiviral expression Gateway cloning destination vector under a hPGK promoter for expression of a gene of interest linked to hygromycin selection by an IRES.

Depositor

Samuel McBrayer

Publication

Savani et al Nat Metab. 2026 Apr 29. doi: 10.1038/s42

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available since

April 22, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MSTN

Plasmid

#241319

Purpose

Expresses bovine MSTN (wild-type) in mammalian cells

Depositor

Mathilde Dupont-Nivet

Publication

Gaiani et al Anim Genet. 2026 Feb;57(1):e70073. doi:

Insert

MSTN full gene (MSTN Bovine)

Expression

Mammalian

Promoter

CMV

Available since

Feb. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pDQM045

Plasmid

#247633

Purpose

to generate mRNA for membrane mScarlet3 for zebrafish

Depositor

Gokul Upadhyayula

Publication

Tschanz et al MicroPubl Biol. 2025 Nov 25;2025:10.1791

Insert

2x Lyn Kinase membrane localization sequence

Use

Zebrafish mrna expression using sp6

Tags

mScarlet3

Available since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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SARS-CoV-2 Nucleocapsid

Plasmid

#242452

Purpose

Expresses the SARS-CoV-2 Nucleocapsid protein

Depositor

Benjamin Schuster

Publication

Favetta et al bioRxiv [Preprint]. 2025 Jun 10:2024.10.

Insert

Nucleocapsid protein

Tags

His

Expression

Bacterial

Promoter

T7

Available since

Sept. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pAL004

Plasmid

#233863

Purpose

MoClo-YTK URA multigene plasmid with MCS; Tet systems; pRNR2-rtTA-v16-tADH1; tetO7-pLEU2m-MCS-tENO2

Depositor

Jeff Hasty

Publication

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

pRNR2-rtTA-v16-tADH1; tetO7-pLEU2m-MCS-tENO2

Expression

Yeast

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pQE-meGFP-DLC1

Plasmid

#192048

Purpose

Plasmid expresses DLC1 binding peptide to Talin R8 domain in chimera with meGFP

Depositor

Ionel Popa

Publication

Dahal et al Sci Adv. 2022 Jul 15;8(28):eabl7719. doi

Insert

DLC1 (DLC1 Human)

Expression

Bacterial

Available since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-mGold2t-CAF1-C-10

Plasmid

#231782

Purpose

Visualization of nucleus in mammalian cells using mGold2t

Depositor

Francois St-Pierre

Publication

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

mGold2t-CAF1-C-10 (Chaf1a Mouse)

Tags

mGold2s

Expression

Mammalian

Promoter

CMV

Available since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-mGold2t-Calnexin-N-14

Plasmid

#231776

Purpose

Visualization of ER in mammalian cells using mGold2t

Depositor

Francois St-Pierre

Publication

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

mGold2t-Calnexin-N-14 (CANX Human)

Tags

mGold2s

Expression

Mammalian

Mutation

I474N

Promoter

CMV

Available since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pET-22b(+) pCopA sfgfp

Plasmid

#226374

Purpose

Plasmid carries copper sensor circuit genes for whole-cell sensing of copper ions with native RBS (ribosome binding site).

Depositor

Urartu Seker

Publication

Koksaldi et al Biosens Bioelectron. 2024 Aug 10;264:116

Insert

sfGFP

Tags

6x HisTag

Expression

Bacterial

Promoter

pCopA

Available since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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FIRESFLIP-PBCS-E670

Plasmid

#228701

Purpose

Sensor cassette FIRE2, sensor in backwards orientation, FIRE fluorescent ratiometric sensor

Depositor

Carolyn Sangokoya

Publication

Sangokoya et al Cell Rep Methods. 2025 Jan 11:100960. do

Insert

FIRE2 SENSOR

Use

Synthetic Biology and Unspecified

Expression

Mammalian

Promoter

EF1-alpha

Available since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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sg_Snrpa_i2-ipUSEPR-TR657

Plasmid

#228923

Purpose

Knockdown of Snrpa in mammalian cells

Depositor

Sika Zheng

Publication

Lin et al Neuron. 2024 Jul 3;112(13):2157-2176.e12

Insert

sg_Snrpa_i2 (Snrpa Mouse)

Use

Lentiviral

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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sg_Snrpa_i4-ipUSEPR-TR657

Plasmid

#228924

Purpose

Knockdown of Snrpa in mammalian cells

Depositor

Sika Zheng

Publication

Lin et al Neuron. 2024 Jul 3;112(13):2157-2176.e12

Insert

sg_Snrpa_i4 (Snrpa Mouse)

Use

Lentiviral

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pETM6-T7-VHH-GFPminimizer

Plasmid

#202488

Purpose

Expresses the nanobody GFP minimizer with T7 induction

Depositor

Michael Lynch

Publication

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody GFP minimizer

Tags

6XHis

Expression

Bacterial

Promoter

T7

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pLPR3

Plasmid

#209986

Purpose

Contains Level 0* Part: resistance cassette (ermBL) for the construction of Level 2 plasmids

Depositor

Thorsten Mascher

Publication

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

resistance cassette (ermBL)

Expression

Bacterial

Available since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pCW-CCND1-HA-puro

Plasmid

#212670

Purpose

Express tagged cyclin D1

Depositor

Yumi Konagaya, Tobias Meyer

Publication

Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

Insert

CCND1 (CCND1 Human)

Use

Lentiviral

Tags

HA

Promoter

TRE

Available since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pTn7-SCOUT14.G

Plasmid

#201002

Purpose

pLv1-pUC18R6KT-miniTn7-aad9-sfGFP, SpR, StR, ApR

Depositor

Philip Poole

Publication

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Insert

superfolder GFP

Expression

Bacterial

Promoter

J23104

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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HRH1-DuET

Plasmid

#213312

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Publication

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

HRH1 (HRH1 Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM033_P7_3"His5

Plasmid

#216458

Purpose

3' homology arm for S. pombe genomic integration. Part type 7 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Publication

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

3''his5

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pPBpuro-hM3D-P2Av3-mCherryNLS

Plasmid

#184714

Purpose

Gq DREADD

Depositor

Michiyuki Matsuda

Publication

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

hM3D

Expression

Mammalian

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo6

Plasmid

#203957

Purpose

Cloning plasmid containing the kanamycin resistance gene insert

Depositor

Nathan Crook

Publication

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Kanamycin Resistance Gene

Expression

Plant

Available since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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