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Plasmid#198813PurposeNatural light-gated anion channels. In vivo inhibition of neurons using blue light.DepositorInsert15xUAS::GtACR2::SL2::GFP::let-858 3'UTR
ExpressionWormAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
UPB-pancreas (UPB7)
Plasmid#180787PurposeUnique Projection Barcode (UPB7)-Pancreas for Projection-seqDepositorInserthM4D(Gi) (partial )
UseAAVExpressionMammalianPromoterCAGAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
LbCas12a
Plasmid#188493PurposeExpresses FLAG tagged LbCas12aDepositorInsertCas12a
UseCRISPR and LentiviralExpressionMammalianPromoterEF1aAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-Electra1-P2A-EGFP
Plasmid#184935PurposeExpresses blue fluorescent protein Electra1 and EGFP in mammalian cellsDepositorInsertElectra1-P2A-EGFP
UseAAVExpressionMammalianPromoterCAGAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGPN-TMPRSS2
Plasmid#187484PurposeExpression vector - TMPRSS2DepositorAvailable sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmr101A-cPr-mNeonGreen (KmR)
Plasmid#177209PurposePlasmid with p15A origin for constitutive mNeonGreen expression in E. coliDepositorInsertmNeonGreen
ExpressionBacterialMutationYeast codon optimizedPromoterSynthetic constitutive promoterAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
PdnaK GFP pZa
Plasmid#170073PurposeGFP expression under the control of E. coli dnaK promoterDepositorInsertGreen fluorescent protein
UseSynthetic BiologyExpressionBacterialPromoterPdnaKAvailable sinceSept. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
DHFR-GFP-th-GluA1
Plasmid#173008PurposeFor light regulated release of GluA1 from the endoplasmic reticulum. Driven by synapsin promoter. Thrombin site is included between GFP and GluA1.DepositorInsertDHFR-GFP-th-GluA1
UseLentiviralTagsGFPExpressionMammalianPromoterhuman synapsinAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSynapsin_NLS_GFP_P2A_His6_NR1.
Plasmid#170457PurposeExpresses histidine tagged NR1 in neuronal cellsDepositorPublicationAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti HsATP10B WT
Plasmid#171770Purposetransfer plasmid for lentiviral vector production expressing Hs ATP10B WTDepositorAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_antiCD19_Z3_Gal4VP64
Plasmid#169916PurposeExpression of a synNotch receptor containing antiCD19, a zebrafish Notch core and Gal4VP64.DepositorInsertantiCD19-Notch3-GL4VP64
UseLentiviralExpressionMammalianPromoterPGKAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPR v2-sgACSL4 clone2
Plasmid#162129PurposeLentiviral sgRNA plasmid targeting human ACSL4DepositorInsertsgACSL4 (ACSL4 Human)
UseLentiviralAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPR v2-sgACSL4 clone1
Plasmid#162128PurposeLentiviral sgRNA plasmid targeting human ACSL4DepositorInsertsgACSL4 (ACSL4 Human)
UseLentiviralAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJW1836
Plasmid#154337PurposePromoter reporterDepositorInsertSV40 NLS::mScarlet_I::PEST (dpi)::-tbb-2 3'UTR
ExpressionWormMutationSilent mutations to remove piRNA sitesAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHelper-T7IS1
Plasmid#140631PurposeExpresses ShCAST under the T7 promoter and an empty sgRNADepositorInsertShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA targeting IS1 (Escherichia coli)
UseCRISPR; TransposonExpressionBacterialAvailable sinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
VQR variant
Plasmid#138560PurposeExpresses human codon-optimized SpCas9-VQR and blasticidin resistance: EFS promoter-VQR-NLS-FLAG-P2A-BSDDepositorInsertVQR
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationD1135V, R1335Q, T1337RPromoterEFSAvailable sinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSCBE2
Plasmid#133965PurposeExpresses BE2 CRISPR base editor and gRNA scaffold in StreptomycesDepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBE2
UseCRISPR and Synthetic BiologyAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Flag-LmoCas6
Plasmid#126485PurposeEncodes L.monocytogenes CRISPR Type I-B Cas6 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLmoCas6
UseCRISPRTagsFlag, NLSExpressionMammalianPromoterCMV and U6Available sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
EF1a_KLF16_P2A_Hygro_Barcode
Plasmid#120502PurposeBarcoded lentiviral vector to express KLF16 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorAvailable sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
EF1a_MYOG_P2A_Hygro_Barcode
Plasmid#120465PurposeBarcoded lentiviral vector to express MYOG in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only