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pMRMTK-clo8

Plasmid

#203959

Purpose

Cloning plasmid containing the spectinomycin resistance gene insert

Depositor

Nathan Crook

Publication

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Spectinomycin Resistance Gene

Expression

Plant

Promoter

N/A

Available since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo10

Plasmid

#203961

Purpose

Cloning plasmid containing the erythromycin resistance gene insert

Depositor

Nathan Crook

Publication

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Erythromycin Resistance Gene

Expression

Plant

Promoter

N/A

Available since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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hPRL3_1-169

Plasmid

#209796

Purpose

Bacterial expression of human PRL-3 without farnesylation motif

Depositor

Kalle Gehring

Publication

Kozlov et al J Biol Chem. 2004 Mar 19. 279(12):11882-

Insert

PRL-3 (PTP4A3 Human)

Tags

6xHis

Expression

Bacterial

Mutation

aa 1-169 only

Promoter

T7

Available since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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OR1-consensus

Plasmid

#208059

Purpose

Consensus OR1 engineered from human OR1 family members

Depositor

Hiroaki Matsunami

Publication

Ikegami et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

Insert

OR1-consensus

Tags

Rhodopsin tag

Expression

Mammalian

Promoter

CMV

Available since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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OR2-consensus

Plasmid

#208060

Purpose

Consensus OR2 engineered from human OR2 family members

Depositor

Hiroaki Matsunami

Publication

Ikegami et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

Insert

OR2-consensus

Tags

Rhodopsin tag

Expression

Mammalian

Promoter

CMV

Available since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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OR4-consensus

Plasmid

#208061

Purpose

Consensus OR4 engineered from human OR4 family members

Depositor

Hiroaki Matsunami

Publication

Ikegami et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

Insert

OR4-consensus

Tags

Rhodopsin tag

Expression

Mammalian

Promoter

CMV

Available since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pGL2_164

Plasmid

#199096

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Publication

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pIP501 ORI plus NGS barcode 4, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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NUDT16

Plasmid

#155794

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Publication

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

NUDT16 (NUDT16 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pDendra2-Hygro

Plasmid

#202407

Purpose

Dendra2 expression plasmid

Depositor

Morito Kurata

Publication

Sugita et al Commun Biol. 2023 Jun 1;6(1):582. doi: 1

Insert

Hygromycin

Tags

Dendra2

Expression

Mammalian

Promoter

CMV

Available since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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Lyn11C3S-EGFP

Plasmid

#202747

Purpose

weakly plasma membrane targeted fluorescent protein

Depositor

Gerry Hammond

Publication

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

LYN (1-11) (C3S):EGFP (LYN Human)

Expression

Mammalian

Available since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pGL2_177

Plasmid

#199109

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Publication

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pBC1 ORI plus NGS barcode 25, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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pGL2_174

Plasmid

#199106

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Publication

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pNG2 ORI plus NGS barcode 22, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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pGL2_165

Plasmid

#199097

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Publication

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pSC101ts ORI plus NGS barcode 10, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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pGL2_161

Plasmid

#199093

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Publication

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pAL5000 ORI plus NGS barcode 1, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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pGL2_162

Plasmid

#199094

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Publication

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pUB110 ORI plus NGS barcode 2, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pJUMP24_T24_pRham-ilvA-relE

Plasmid

#201531

Purpose

Expressing synthetic overlapping gene, ilvA-relE. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)

Depositor

Dan Park

Publication

Chlebek et al Nucleic Acids Res. 2023 Jun 1:gkad484. d

Insert

ilvA-relE

Use

Synthetic Biology

Promoter

PrhaBAD (rhamnose)

Available since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

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UG75-ADH1-CDS1-3HA

Plasmid

#198596

Purpose

CDS1 overexpression. Diverts glycerolipid synthesis pathway towards phospholipids. Uses antibiotic resistance marker hphMX6.

Depositor

Zhiping Xie

Publication

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

CDS1 (CDS1 Budding Yeast)

Tags

3HA

Expression

Yeast

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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UG75-ADH1-PAH1-3HA

Plasmid

#198597

Purpose

PAH1 overexpression. Promotes the formation of diacylglycerol from phosphatidate. Uses antibiotic resistance marker hphMX6.

Depositor

Zhiping Xie

Publication

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

PAH1 (PAH1 Budding Yeast)

Tags

3HA

Expression

Yeast

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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ABF2-2EGFP-ter-URA3

Plasmid

#184768

Purpose

Marker for yeast mitochondria. Integration of 2xGFP tag at ABF2 C terminus. Uses auxotrophic marker URA3(Candida albicans).

Depositor

Zhiping Xie

Publication

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

ABF2 (ABF2 Budding Yeast)

Tags

2EGFP

Expression

Yeast

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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LLP747_L2pV1_OR_B3RT_FRT_Rluc_2IBF_lacZ

Plasmid

#192391

Purpose

To act as a 2-input state of the OR gate.

Depositor

Ryan Lister

Publication

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-B3RT-OCS-FRT-B3RT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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