We narrowed to 64,737 results for: cell;

1. pU6-TREX1 g1 Cas9-T2A-mCherry

   Plasmid

   #164250

   Purpose

   Transcription of TREX1 guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells

   Depositor

   John Maciejowski

   Article

   Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

   Insert

   sgTREX1
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. p1.2-Hygro

   Plasmid

   #162741

   Purpose

   Protein expression in CHO cells

   Depositor

   Ivan Vorobiev

   Article

   Orlova et al BMC Biotechnol. 2014 Jun 14;14:56. doi:

   Type

   Empty backbone

   Expression

   Mammalian

   Promoter

   CHO Elongation factor 1A

   Available Since

   July 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pACEBac1-GCP3

   Plasmid

   #178069

   Purpose

   Insect cell expression of GCP3

   Depositor

   Tarun Kapoor

   Article

   Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

   Insert

   GCP3 (TUBGCP3 Human)
   

   Expression

   Insect

   Available Since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. p1.2-Neo

   Plasmid

   #162739

   Purpose

   Protein expression in CHO cells

   Depositor

   Ivan Vorobiev

   Article

   Orlova et al BMC Biotechnol. 2014 Jun 14;14:56. doi:

   Type

   Empty backbone

   Expression

   Mammalian

   Promoter

   CHO Elongation factor 1A

   Available Since

   July 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pmOrange 2-Actin

   Plasmid

   #199703

   Purpose

   visualization of cleavage furrow in living mammalian mitotic cells

   Depositor

   Kenji Sugimoto

   Article

   Tanaka et al Bioimages 25: 7-19, 2017

   Insert

   human cytoplasmic action
   

   Tags

   mOrange 2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. Ac5-STABLE2-blast

   Plasmid

   #208034

   Purpose

   Enables expression of 2 ORFs (with or without tags) and selection with blasticidin in insect cells. Includes Flag-mCherry-T2A-GFP-T2A-blast with Actin5C promoter.

   Depositor

   Rosa Barrio, James Sutherland

   Article

   Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

   Type

   Empty backbone

   Expression

   Insect

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. YIplac211-HaloTag-DRS2

   Plasmid

   #177103

   Purpose

   Expresses HaloTag-Drs2 in yeast cells

   Depositor

   Benjamin Glick

   Article

   Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

   Insert

   DRS2 (DRS2 Budding Yeast)
   

   Expression

   Yeast

   Available Since

   Aug. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLH-stsgRNA2.1

   Plasmid

   #64117

   Purpose

   Vector for expression of St1 sgRNA2.1 in mammalian cells

   Depositor

   Thoru Pederson

   Article

   Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

   Insert

   St1 sgRNA2.1
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. YIplac211-NHX1-HaloTagx2

   Plasmid

   #177107

   Purpose

   Expresses Nhx1-HaloTagx2 in yeast cells

   Depositor

   Benjamin Glick

   Article

   Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

   Insert

   NHX1 (NHX1 Budding Yeast)
   

   Expression

   Yeast

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHAGE-SSFV-dCas9-GFP

    Plasmid

    #64105

    Purpose

    Expression of Sp dCas9-GFP in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    Sp dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    sfGFP

    Expression

    Mammalian

    Promoter

    SSFV

    Available Since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-Flag-YOD1 C160S

    Plasmid

    #49785

    Purpose

    Express YOD1 C160S mutant with a N-terminal Flag-tag in mammalian cells

    Depositor

    Christian Schlieker

    Article

    Ernst et al Mol Cell. 2009 Oct 9;36(1):28-38. doi: 1

    Insert

    YOD1 (YOD1 Human)
    

    Tags

    Flag-tag

    Mutation

    C160S

    Available Since

    Dec. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

12. YIplac211-ANP1-msGFP2

    Plasmid

    #177100

    Purpose

    Expresses Anp1-msGFP2 in yeast cells

    Depositor

    Benjamin Glick

    Article

    Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

    Insert

    ANP1 (ANP1 Budding Yeast)
    

    Expression

    Yeast

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. Cdc2-DN

    Plasmid

    #1887

    Depositor

    Sander van den Heuvel

    Article

    van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

    Insert

    Cdc2 (CDK1 Human)
    

    Expression

    Mammalian

    Mutation

    D146N, dominant negative

    Available Since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

14. pME-puro-CLIP-FLAG-CD59

    Plasmid

    #50375

    Purpose

    Express CLIP and FLAG-tagged human CD59, a GPI-AP in mammmalian cells

    Depositor

    Reika Watanabe

    Article

    Castillon et al Mol Biol Cell. 2013 Jun;24(12):2021-33.

    Insert

    CLIP
    

    Expression

    Mammalian

    Promoter

    SR alpha

    Available Since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMX1418

    Plasmid

    #127856

    Purpose

    Expresses EGFP-IPNN-MCAK in mammalian cells

    Depositor

    Linda Wordeman

    Article

    Domnitz et al J Cell Biol. 2012 Apr 16;197(2):231-7. d

    Insert

    IPNN-MCAK
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    mutation is I97N and P98N

    Promoter

    CMV

    Available Since

    July 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAc-sgRNA-Cas9.scr_2

    Plasmid

    #190704

    Purpose

    Expresses scrambled sgRNA and Cas9-Puro in Drosophila S2 cells

    Depositor

    David Bartel

    Article

    Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

    Insert

    Scrambled sgRNA 2
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    Drosophila U6

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLH-stsgRNA1.1

    Plasmid

    #64116

    Purpose

    Vector for expression of St1 sgRNA1.1 in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    St1 sgRNA1.1
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX165-xCas9

    Plasmid

    #140568

    Purpose

    Expresses xCas9 in mammalian cells

    Depositor

    Feng Zhang

    Article

    Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

    Insert

    Cas9
    

    Mutation

    A262T R324L S409I E480K E543D M694I E1219V

    Available Since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. 7iBi89 (rat fibronectin)

    Plasmid

    #14065

    Depositor

    Richard Hynes

    Article

    Huh et al Mol Cell Biol. 1993 Sep . 13(9):5301-14.

    Insert

    Rat Fibronectin 7iBi89 (Fn1 Rat)
    

    Expression

    Mammalian

    Mutation

    Insert was cut out of pGEM3 with SalI and SacI an…

    Available Since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

20. mCherry-INPP5J

    Plasmid

    #171949

    Purpose

    Expresses INPP5J in mammalian cells

    Depositor

    Cheng-han Yu

    Article

    Feng et al Proc Natl Acad Sci U S A. 2021 May 11;11

    Insert

    INPP5J (INPP5J Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    July 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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