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pTwist-SARS-CoV-2 Spike-deltaC18 Y453F (TTC)

Plasmid

#212369

Purpose

Encodes SARS-CoV-2 spike Y453F for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike Y453F (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

Y453F

Promoter

CAG

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 BA.5

Plasmid

#212505

Purpose

Encodes SARS-CoV-2 variant BA.5 Spike for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike BA.5 (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

See Depositor Comments.

Promoter

CAG

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 F456L (CTT)

Plasmid

#212542

Purpose

Encodes SARS-CoV-2 Spike F456L for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike F456L (CTT) (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

F456L

Promoter

CAG

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 FL.1.5.1

Plasmid

#212539

Purpose

Encodes SARS-CoV-2 variant FL1.5.1 Spike for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike FL.1.5.1 (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

See Depositor Comments.

Promoter

CAG

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pMV-TC1-2_DM

Plasmid

#217081

Purpose

Donor plasmid of TC1-2_DM Transposon

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

TIRs of TC1-2_DM transposon

Expression

Mammalian

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pMV-Tc1-8_Xt

Plasmid

#217092

Purpose

Donor plasmid of Tc1-8_Xt Transposon

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

TIRs of Tc1-8_Xt transposon

Expression

Mammalian

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pC368: pAAV.CMV-Cas13e-VEGFA sgRNA2

Plasmid

#227800

Purpose

Plasmid expressing active Cas13e with VEGFA mRNA targeting gRNA2

Depositor

Guei-Sheung Liu

Article

Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

Inserts

U6-VEGFA sgRNA 2 (Vegfa Synthetic)
Cas13e

Use

AAV

Tags

HA and NLS

Expression

Mammalian

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pC369: pAAV.CMV-Cas13e-VEGFA sgRNA3

Plasmid

#227801

Purpose

Plasmid expressing active Cas13e with VEGFA mRNA targeting gRNA3

Depositor

Guei-Sheung Liu

Article

Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

Inserts

U6-VEGFA sgRNA 3 (Vegfa Synthetic)
Cas13e

Use

AAV

Tags

HA and NLS

Expression

Mammalian

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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Cdk13_intron3_sg3_pX458

Plasmid

#127338

Purpose

sgRNA that cuts within intron 3 of mouse CDK13 genomic locus- guide #3

Depositor

Phil Sharp

Article

Insco et al Science. 2023 Apr 21;380(6642):eabn7625.

Insert

Mouse Cdk13 Intron 3 sgRNA

Use

CRISPR and Mouse Targeting

Promoter

U6 Promoter

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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Cdk13_intron4_sg4_pX330

Plasmid

#127341

Purpose

sgRNA that cuts within intron 4 of mouse CDK13 genomic locus- guide #4

Depositor

Phil Sharp

Article

Insco et al Science. 2023 Apr 21;380(6642):eabn7625.

Insert

Mouse Cdk13 Intron 4 Targeting sgRNA

Use

CRISPR and Mouse Targeting

Promoter

U6 Promoter

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pMV-Mariner-2_XT

Plasmid

#217096

Purpose

Donor plasmid of Mariner-2_XT Transposon

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

TIRs of Mariner-2_XT transposon

Expression

Mammalian

Available Since

Dec. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 Q183H (CAT)

Plasmid

#223279

Purpose

Encodes SARS-CoV-2 spike Q183H for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike Q183H (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

Q183H

Promoter

CAG

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pMV-Tc1-2_ST-10 kb

Plasmid

#218314

Purpose

Donor plasmid of Tc1-2_ST Transposon with 10 kb cargo gene

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

10 kb cargo gene

Expression

Mammalian

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pQLL-MAG-10 kb

Plasmid

#218315

Purpose

Donor plasmid of MAG Transposon with 10 kb cargo gene

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

10 kb cargo gene

Expression

Mammalian

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pQLL-Tc1-8B_DR-10 kb

Plasmid

#218318

Purpose

Donor plasmid of Tc1-8B_DR Transposon with 10 kb cargo gene

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

10 kb cargo gene

Expression

Mammalian

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pMV-Tc1-1_PM-10 kb

Plasmid

#218319

Purpose

Donor plasmid of Tc1-1_PM Transposon with 10 kb cargo gene

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

10 kb cargo gene

Expression

Mammalian

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pMV-piggyBac-1_AMi-10 kb

Plasmid

#218320

Purpose

Donor plasmid of piggyBac-1_AMi Transposon with 10 kb cargo gene

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

10 kb cargo gene

Expression

Mammalian

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pMV-SB100X-10 kb

Plasmid

#218313

Purpose

Donor plasmid of SB100X Transposon with 10 kb cargo gene

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

10 kb cargo gene

Expression

Mammalian

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pQLL-MAG w/o spacer

Plasmid

#218292

Purpose

Donor plasmid of MAG Transposon without spacer

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

TIRs of MAG Transposon

Expression

Mammalian

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pRRLSIN-[EF1α-CD19 4-1BB]

Plasmid

#218340

Purpose

Lentiviral transfer plasmids for mammalian expression CD19 CAR

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

CD19 CAR mammalian expression cassette

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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