We narrowed to 25,030 results for: Nes

1. pCMV-AAV2cap

   Plasmid

   #198014

   Purpose

   For high-level expression of the AAV2 cap gene, suitable for use with pCMV-Rep78/68 SSM library

   Depositor

   George Church

   Article

   Jain et al bioRxiv 2023.01.31.526541

   Insert

   AAV2 cap
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available Since

   May 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCK122 pDESTattB_exorh:mCherry

   Plasmid

   #213511

   Purpose

   attB-containing pDEST plasmid, for use in Multisite Gateway reaction

   Depositor

   Christian Mosimann

   Article

   Lalonde et al Sci Adv. 2024 Jun 7;10(23):eadn6603. doi

   Type

   Empty backbone

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSBtet-GP

   Plasmid

   #60495

   Purpose

   SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of GFP, rtTA and puromycin resistance gene

   Depositor

   Eric Kowarz

   Article

   Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TCE & RPBSA

   Available Since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGFP-Rab8A

   Plasmid

   #24898

   Depositor

   Maxence Nachury

   Article

   Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

   Insert

   hRab8A
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 17, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHO4d-Cas9

   Plasmid

   #67881

   Purpose

   Cas9nlsHis6 Bacterial Expression construct

   Depositor

   Michael Nonet

   Article

   Fu et al Nucleic Acids Res. 2014 Dec 16;42(22):13

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   His6 and T7 epitope

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Sept. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-Tol2

   Plasmid

   #31823

   Depositor

   Stephen Ekker

   Article

   Balciunas et al PLoS Genet. 2006 Nov 10;2(11):e169. Epub

   Insert

   Tol2 Transposase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB-TO-MYOD1-shOct4

   Plasmid

   #182309

   Purpose

   Piggybac Tet-ON plasmid for differentiating hiPSCs into skeletal muscle cells via MYOD1 and shOCT4 expression

   Depositor

   Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

   Inserts

   MYOD1-shOct4 (MYOD1 Human)
   puro-T2A-mycNLS-mTagBFP2
   

   Use

   Tags

   T2A-mycNLS-mTagBFP2

   Expression

   Mammalian

   Mutation

   shOct4

   Promoter

   EF1a and TRE3G

   Available Since

   April 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLCNICK

   Plasmid

   #84653

   Purpose

   Genome editing for Lactobacillus casei Lc2W

   Depositor

   Sheng Yang

   Article

   Song et al Appl Environ Microbiol. 2017 Sep 1. pii:

   Inserts

   Cas9 nickase
   sgRNA
   homology arms of LC2W_2179
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A

   Promoter

   Available Since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCherry-PTDSS1-IRES-puromycin-pLVx-EF1a

   Plasmid

   #177435

   Purpose

   To express mCherry fused to PTDSS1, a protein that localized to mitochondria associated ER membranes (MAMs). Lentiviral vector used to make cell lines expressing this MAM landmark.

   Depositor

   David Andrews

   Insert

   Phosphatidylserine synthase-1, PTDSS1, LMHD, PSS1, PSSA (PTDSS1 Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pVS1-SAH2

    Plasmid

    #134746

    Purpose

    Replication helper

    Depositor

    Qi-Jun Chen

    Article

    Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

    Insert

    trfA-trfB-parDE
    

    Use

    Helper plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pVS1-VIR2

    Plasmid

    #134745

    Purpose

    Virulence and replication helper

    Depositor

    Qi-Jun Chen

    Article

    Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

    Insert

    vir gene cluster
    

    Use

    Vir gene expression

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCB301-2μ-HDV

    Plasmid

    #196329

    Purpose

    A yeast-Escherichia coli-Agrobacterium shuttle vector

    Depositor

    Zhenghe Li

    Article

    Sun et al Viruses. 2017 Nov 7;9(11):332. doi: 10.3

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGL0_1 [RK2 ORI]

    Plasmid

    #198918

    Purpose

    Level 0 part

    Depositor

    Nili Ostrov, Cultivarium Tools, Charlie Gilbert

    Article

    Gilbert et al bioRxiv 2023.05.19.541510

    Insert

    RK2 ORI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCFJ90 - Pmyo-2::mCherry::unc-54utr

    Plasmid

    #19327

    Depositor

    Erik Jorgensen

    Article

    Christian et al

    Insert

    Pmyo-2::mCherry
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    Oct. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSBbi-Neo

    Plasmid

    #60525

    Purpose

    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: neomycin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSBbi-Hyg

    Plasmid

    #60524

    Purpose

    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: hygromycin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. PKC alpha WT

    Plasmid

    #21232

    Depositor

    Bernard Weinstein

    Article

    Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

    Insert

    PKC alpha (PRKCA Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX165-HiFi Cas9

    Plasmid

    #140563

    Purpose

    Expresses HiFi Cas9 in mammalian cells

    Depositor

    Feng Zhang

    Article

    Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Mutation

    R691A

    Promoter

    Available Since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAMBIA-FLS2prom::FLS2-3xmyc-GFP

    Plasmid

    #86157

    Purpose

    FLS2-GFP expresssion

    Depositor

    Silke Robatzek

    Article

    Robatzek et al Genes Dev. 2006 Mar 1;20(5):537-42.

    Insert

    FLS2 (FLS2 Mustard Weed)
    

    Use

    Tags

    3xmyc and GFP

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDD426

    Plasmid

    #202079

    Purpose

    Frame +1/-0 selector for NHEJ knock-in

    Depositor

    Daniel Dickinson

    Article

    Shi et al Development. 2023 May 15;150(10):dev2013

    Insert

    pEF1a-mCherry-Cas9 + Frame +1/-0 sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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