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pLV[Exp]-Hygro-CMV-FUCCIplex

Plasmid

#240221

Purpose

Multiplexable FUCCI sensor (FUCCIplex). The CMV promoter drives the expression of the fluorescent sensor.

Depositor

Francesco Pasqualini

Article

Di Sante et al bioRxiv 2024.12.19.629259

Insert

FUCCIplex

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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mTagRFP-T-Endosomes-14

Plasmid

#58014

Purpose

Localization: Endosomes, Excitation: 555, Emission: 584

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

Endosomes (RHOB Human)

Tags

mTagRFP

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2015

Availability

Industry, Academic Institutions, and Nonprofits
gTEMP_pcDNA3

Plasmid

#89583

Purpose

Genetically encoded ratiometric fluorescent thermometer

Depositor

Takeharu Nagai

Article

Nakano et al PLoS One. 2017 Feb 17;12(2):e0172344. do

Insert

gTEMP

Expression

Mammalian

Promoter

CMV

Available Since

May 5, 2017

Availability

Academic Institutions and Nonprofits only

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mWasabi-C1

Plasmid

#54743

Purpose

Localization: C1 Cloning Vector, Excitation: 493, Emission: 509

Depositor

Robert Campbell, Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Type

Empty backbone

Tags

mWasabi

Expression

Mammalian

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pHK326

Plasmid

#235475

Purpose

Inducible gene VIII by Ptac promoter

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

phage M13 geneVIII

Expression

Bacterial

Available Since

May 9, 2025

Availability

Academic Institutions and Nonprofits only

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HP17deltaP8

Plasmid

#235447

Purpose

Helper plasmid for regulated phage transfer

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

Coding part of the M13KO7 helper phage genome, except gene VIII

Expression

Bacterial

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pfMDH_WT

Plasmid

#67721

Purpose

Expresses WT mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

Depositor

Justin Siegel

Article

Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

Insert

Mannitol 2-Dehydrogenase from Pseudomonas fluorescens

Tags

6x-His tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only
mWasabi-N1

Plasmid

#54765

Purpose

Localization: N1 Cloning Vector, Excitation: 493, Emission: 509

Depositor

Robert Campbell, Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Type

Empty backbone

Tags

mWasabi

Expression

Mammalian

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only
pNCS YFP

Plasmid

#91759

Purpose

Bacterial expression of a fluorescent protein, Yellow Fluorescent Protein (YFP)

Depositor

Erik Rodriguez, Roger Tsien

Article

Cubitt et al Trends Biochem Sci. 1995 Nov;20(11):448-

Insert

Venus YFP (A206K)

Tags

Hexa-Histidine tag, Xpress epitope for detection …

Expression

Bacterial

Promoter

T7

Available Since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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pET24_NusA-Tus

Plasmid

#165959

Purpose

Expression of soluble E. coli Tus protein

Depositor

Keith Pardee

Article

Norouzi et al ACS Synth Biol. 2021 Jun 23. doi: 10.102

Insert

Tus

Tags

His-NusA-Tev

Expression

Bacterial

Promoter

T7

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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mEmerald-Vinexin-C-14

Plasmid

#54305

Purpose

Localization: Focal Adhesions, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

Vinexin (SORBS3 Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits
mTFP1-C1

Plasmid

#54613

Purpose

Localization: C1 Cloning Vector, Excitation: 462, Emission: 492

Depositor

Robert Campbell, Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Type

Empty backbone

Tags

mTFP1

Expression

Mammalian

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

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mKusabira Orange-C1 (mKO)

Plasmid

#54834

Purpose

Localization: C1 Cloning Vector, Excitation: 548, Emission: 561

Depositor

Michael Davidson, Atsushi Miyawaki

Article

Karasawa et al Biochem J. 2004 Jul 1. 381(Pt 1):307-12.

Type

Empty backbone

Tags

mKusabira Orange

Expression

Mammalian

Available Since

June 12, 2014

Availability

Academic Institutions and Nonprofits only

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MiCy-pBAD

Plasmid

#54565

Purpose

Localization: Protein Expression Vector , Excitation: 472, Emission: 495

Depositor

Michael Davidson, Atsushi Miyawaki

Article

Karasawa et al Biochem J. 2004 Jul 1. 381(Pt 1):307-12.

Type

Empty backbone

Tags

6xHis and MiCy

Expression

Bacterial

Available Since

July 25, 2014

Availability

Academic Institutions and Nonprofits only
mCyRFP1-Cdc42

Plasmid

#84360

Purpose

fusion protein of Cdc42, FRET donor

Depositor

Ryohei Yasuda

Article

Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

mCyRFP1-Cdc42

Tags

mCyRFP1 N terminal fusion to Cdc42

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-URA-GAL1-HALO-3xHA

Plasmid

#188937

Purpose

Integration cassette to create N-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

URA-GAL1-HALO-3xHA

Expression

Yeast

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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csr-1_son-1_mNeonGreen

Plasmid

#191743

Purpose

Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.

Depositor

Jay C Dunlap

Article

Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

Insert

NCU04288 (NCU04288 Neurospora crassa)

Tags

NCU04502 promoter and mNeonGreen

Expression

Bacterial

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pJOE7771.1

Plasmid

#135077

Purpose

Mannitol, arabitol, and glucitol-inducible mtlR/PmtlE expression vector

Depositor

Josef Altenbuchner

Article

Hoffmann et al PLoS One. 2015 Jul 24;10(7):e0133248. do

Insert

mtlR

Expression

Bacterial

Available Since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pHK316

Plasmid

#235474

Purpose

Inducible gene VIII by Ptet promoter

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

phage M13 geneVIII

Expression

Bacterial

Available Since

May 9, 2025

Availability

Academic Institutions and Nonprofits only

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mAzami Green-N1 (mAG)

Plasmid

#54798

Purpose

Localization: N1 Cloning Vector, Excitation: 492, Emission: 505

Depositor

Michael Davidson, Atsushi Miyawaki

Article

Karasawa et al J Biol Chem. 2003 Sep 5. 278(36):34167-7

Type

Empty backbone

Tags

mAzami Green

Expression

Mammalian

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only