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Plasmid#44021DepositorInsertHRP
UseTagsHA and c-mycExpressionYeastMutationPromoterAvailable sinceMarch 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJYDN
Plasmid#162450PurposeYeast display plasmid intended for expression of N-terminal fusions with Aga2p yeast agglutinin and eUnaG2 bilirubin dependent green-yellow fluorescent protein for detection of expression.DepositorTypeEmpty backboneUseShuttle vector bacteria/yeastTagsAga2p, HA, myc tags, and eUnaG2 fluorescent prote…ExpressionYeastMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pCTCON2-Aga1p
Plasmid#158141PurposeExpresses Aga1p in yeastDepositorInsertAga1p
UseTagsExpressionYeastMutationPromoterGAL10Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
p414ADH_Hsp82_CycTER
Plasmid#61712Purposeyeast plasmid with Hsp82DepositorInsert6xHis-Hsp82 (HSP82 Budding Yeast)
UseTags6xHisExpressionYeastMutationPromoterADH1Available sinceJan. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pETconNK
Plasmid#81169PurposepETconNK Empty BackboneDepositorTypeEmpty backboneUseTagsc-mycExpressionBacterial and YeastMutationPromoterGAL1Available sinceFeb. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJYDC3
Plasmid#162460PurposeYeast display plasmid for expression of C-terminal fusions with Aga2p yeast agglutinin. The reporter DnbALFA was fused to the N-terminus of Aga2p.DepositorTypeEmpty backboneUseShuttle vector bacteria/yeastTagsDnbALFA designed ALFA nanobody, HA tag, HDEL sequ…ExpressionMutationPromoterAvailable sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS424GFP-2xPH(Osh2)
Plasmid#36095DepositorInsert2xPH(Osh2) fusion
UseTagsGFPExpressionYeastMutationPromoterPRC1Available sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pJYDN3
Plasmid#162456PurposeYeast display platform plasmid N3 which allows for simultaneous expression of the protein of interest at the N-terminus of Aga2p yeast agglutinin and eUnaG2 or nbALFA detection.DepositorTypeEmpty backboneUseShuttle vector bacteria/yeastTagsALFA tag, HA, myc tags, and eUnaG2 fluorescent pr…ExpressionMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTPGI_dCas9
Plasmid#64279Purposeencodes yeast-optimized dCas9 synthetic transcription factorDepositorInsertYeast-optimized dCas9
UseTagsSV40 NLSExpressionYeastMutationD10A & H840A relative to S. pyogenes Cas9PromoterpTPGIAvailable sinceMay 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJYDN2
Plasmid#162454PurposeYeast display plasmid intended for co-cultivation labeling by using designed ALFA-tag binding nanobody (DnbALFA) and expression of N-terminal fusions with Aga2p yeast agglutinin.DepositorTypeEmpty backboneUseShuttle vector bacteria/yeastTagsDnbALFA nanobody and Ha and mycExpressionMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
YIplac204-TC-OM45-FKBPx4
Plasmid#104472PurposeIntegrating plasmid for overexpression of OM45-FKBPx4.DepositorInsertOM45p (OM45 Budding Yeast)
UseTagsFKBPx4- HAExpressionYeastMutationPromoterTPI1Available sinceFeb. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
LHP1463 - INP52
Plasmid#32432DepositorInsertINP52 (INP52 Budding Yeast)
UseTagsExpressionYeastMutationPromoterLacAvailable sinceNov. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCUP1pNuiHA kanMX CEN
Plasmid#131168Purposefor ScCUP1 promoter regulated N-terminal fused Nui-HA prey expression, centromeric ARS plasmid, kanMX marker confers resistance to geneticin in S. cerevisiaeDepositorTypeEmpty backboneUseTagsNui-HAExpressionYeastMutationPromoterAvailable sinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
YIPlac204TC-NLS-DsRed-Express2
Plasmid#25443DepositorInsertNLS-DsRed-Express2
UseTagsNLSExpressionYeastMutationPromoterAvailable sinceJuly 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
pHES875
Plasmid#89192PurposeExpresses mCherry under control of GAL1 promoter.DepositorInsertpGAL1-mCherry
UseTagsExpressionYeastMutationPromoterGAL1Available sinceJan. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMLB10
Plasmid#177273PurposeGAL1p:T7RNAP(F11L;T613A):CYC1tDepositorInsertT7RNAP(F11L;T613A)
UseTagsExpressionBacterial and YeastMutationF11L T613APromoterGAL1Available sinceApril 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
YIplac204-TC-OM45-FKBPx2
Plasmid#104471PurposeIntegrating plasmid for overexpression of OM45-FKBPx2.DepositorInsertOM45p (OM45 Budding Yeast)
UseTagsFKBPx2- HAExpressionYeastMutationPromoterTPI1Available sinceFeb. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHES820
Plasmid#89190PurposeExpresses YFP under control of GAL1 promoter.DepositorInsertpGAL1-YFP
UseTagsExpressionYeastMutationPromoterGAL1Available sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only