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pHR-CMV-TetO2_3C-mVenus-Twin-Strep

Plasmid

#113891

Purpose

Mammalian (inducible) protein expression, C-terminal 3C-mVenus-Twin-Strep fusion

Depositor

A. Radu Aricescu

Publication

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-mVenus-Twin-Strep

Promoter

CMV-MIE-TetO2

Available since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCMV6-AC 4-μHOM

Plasmid

#113619

Purpose

Reporter for Alt-EJ, variant of EJ7-GFP. Double strand breaks are induced with the 7a & 7h, or 7a & 7i sgRNAs, with CAS9. EJ using 4 nt of microhomology causes a deletion mutation and restores GFP

Depositor

Jeremy Stark

Publication

Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

Insert

4-μHOM variant of EGFP

Expression

Mammalian

Available since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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Human USP30 (1-517, WT, MG-38-11)

Plasmid

#110748

Purpose

Transient transfection of human USP30

Depositor

David Komander

Publication

Gersch et al Nat Struct Mol Biol. 2017 Sep 25. doi: 1

Insert

USP30 (USP30 Human)

Expression

Mammalian

Promoter

CMV

Available since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CWB-pre-eGRASP(p30)

Plasmid

#111597

Purpose

An AAV vector that expresses green pre-eGRASP(p30) under the CaMKIIa promoter.

Depositor

Bong-Kiun Kaang

Publication

Choi et al Science. 2018 Apr 27;360(6387):430-435.

Insert

green pre-eGRASP(p30)

Use

AAV

Promoter

CaMKIIa

Available since

June 12, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCas9-Geminin

Plasmid

#109401

Purpose

Expresses Cas9 fusion to Geminin degron domain

Depositor

Jean-Paul Concordet

Publication

Charpentier et al Nat Commun. 2018 Mar 19;9(1):1133. doi:

Insert

Geminin (GMNN Human)

Mutation

Geminin degron fragment

Available since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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VEGFR2-YFP

Plasmid

#108853

Purpose

Encodes for human VEGFR2 fluorescently labeled with eYFP on the C-Terminus via a 3 amino acid (GGS) flexible linker

Depositor

Kalina Hristova

Publication

Sarabipour et al Elife. 2016 Apr 7;5:e13876. doi: 10.7554

Insert

KDR (KDR Human)

Tags

Labeled with eYFP on the C-Terminus via a 3 amino…

Expression

Mammalian

Available since

May 10, 2018

Availability

Academic Institutions and Nonprofits only

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LentiRCas9-Lambda2

Plasmid

#104184

Purpose

Lentiviral transfer vector that carries U6-driven non-targeting sgRNA using a modified scaffold (Chen et al. Cell 2013) and CMV-driven PIN-dCas9. Derived from LentiCRISPR v2 (Zhang lab)

Depositor

Eugene Yeo

Publication

Batra et al Cell. 2017 Aug 24;170(5):899-912.e10. do

Insert

U6-sgRNA, EFS-PIN-dCas9

Use

CRISPR and Lentiviral

Available since

April 13, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-hSyn-ASAP2s

Plasmid

#101276

Purpose

Genetically encoded voltage indicator (GEVI) ASAP2s in AAV vector for neuron-specific expression using the promoter hSyn1

Depositor

Francois St-Pierre

Publication

Chamberland et al eLife. 2017 Jul 27;6. pii: e25690. doi:

Insert

ASAP2s

Use

AAV

Expression

Mammalian

Promoter

hSyn (human synapsin)

Available since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pcrDNA3.1-Chimera

Plasmid

#100891

Purpose

Mammalian expression of DNAJB1(exon1)-PRKACA(exon2-Cterminus) chimeric protein from fibrolamellar hepatocellular carcinoma

Depositor

Sanford Simon

Publication

Honeyman et al Science. 2014 Feb 28;343(6174):1010-4. d

Insert

Major Fibrolamellar hepatocellular carcinoma Chimera (DNAJB1, PRKACA Human)

Expression

Mammalian

Promoter

CMV

Available since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pINDUCER-21-LCOR

Plasmid

#97038

Purpose

Expresses LCOR in mammalian cells

Depositor

George Daley

Publication

Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

Insert

Ligand Dependent Nuclear Receptor Corepressor (LCOR Human)

Use

Lentiviral

Expression

Mammalian

Promoter

Tet on

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pIRES-hrGFP II-TET1_CD

Plasmid

#83570

Purpose

Expresses the catalytic domain of TET1 in mammalian cells

Depositor

Jean-Pierre Issa

Publication

Jin et al Nucleic Acids Res. 2014 Jun;42(11):6956-

Insert

Tet Methylcytosine Dioxygenase 1 (TET1 Human)

Tags

3X FLAG tag and hr GFP II

Expression

Mammalian

Mutation

TET1 amino acids 1-1417 deleted

Promoter

CMV

Available since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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GST-mDia1(FH1FH2DAD)-His

Plasmid

#85822

Purpose

contains the formin homolgy domains: FH2 is required for nucleation, processive tracking of barbed-end of actin filaments ; FH1 permits enhancement of actin polymerization rate.

Depositor

Antoine Jégou, Guillaume Romet-Lemonne

Publication

Jegou et al Nat Commun. 2013;4:1883. doi: 10.1038/nc

Insert

Protein diaphanous homolog 1 (Diaph1 Mouse)

Tags

8*His and GST

Expression

Bacterial

Promoter

tac

Available since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Lenti-sgRNA(MS2)-puro-barcode

Plasmid

#89493

Purpose

Cloning backbone for sgRNA, also has a barcode that allows detection from scRNA-seq

Depositor

Gary Hon

Publication

Xie et al Mol Cell. 2017 Apr 20;66(2):285-299.e5.

Insert

sgRNA (with MS2 loop)

Use

Lentiviral

Promoter

U6

Available since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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hZMPSTE24-3FLAG_pQCXIP

Plasmid

#89759

Purpose

Human ZMPSTE24 mammalian expression vector. Retroviral bicistronic puromycin vector.

Depositor

Martin Dorf

Publication

Fu et al J Exp Med. 2017 Feb 28. pii: jem.2016127

Insert

Homo sapiens zinc metallopeptidase STE24 (ZMPSTE24) (ZMPSTE24 Human)

Use

Retroviral; Bicistronic expression: puromycin re…

Tags

9bp spacer (NotI site) - 3xFLAG epitopes - Stop c…

Expression

Mammalian

Mutation

Y253H in ZMPSTE24 (see depositor comments below)

Promoter

CMV

Available since

June 8, 2017

Availability

Academic Institutions and Nonprofits only

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pJYS2cm_crtYf

Plasmid

#85543

Purpose

Constitutive transcription of crRNA of crtYf, Chloramphenicol resistance

Depositor

Sheng Yang

Publication

Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

Insert

crRNA of crtYf

Use

CRISPR; Shuttle vector corynebacterium glutamicum…

Expression

Bacterial

Available since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-hL1

Plasmid

#89411

Purpose

mammalian expression of hL1

Depositor

Fritz Rathjen

Publication

De Angelis et al EMBO J. 1999 Sep 1. 18(17):4744-53.

Insert

L1CAM (L1CAM Human)

Expression

Mammalian

Promoter

CMV

Available since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNAZeo(-) LAMP2C

Plasmid

#89342

Purpose

Expresses human LAMP-2C in mammalian cells

Depositor

Janice Blum

Publication

Perez et al J Immunol. 2016 Mar 15;196(6):2457-65. d

Insert

Lysosomal-associated membrane protein 2C (LAMP2 Human)

Expression

Mammalian

Promoter

CMV

Available since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA-mCherry-4A/4B-CAAX

Plasmid

#87699

Purpose

Express mCherry with a peptide linker (contaning a HCVp 4A/4B substrate site) and a C-terminal CAAX box from KRras for plasma-membrane targeting in mammalian cells

Depositor

Robert Campbell

Publication

Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

mCherry-4A/4B-CAAX

Expression

Mammalian

Available since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-2/hGGA3(GAT)

Plasmid

#79436

Purpose

Expresses GST-tagged GAT domain of GGA3 (107-286 aa) in E.coli

Depositor

Kazuhisa Nakayama

Publication

Takatsu et al Biochem J. 2002 Jul 15;365(Pt 2):369-78.

Insert

GGA3 (GGA3 Human)

Tags

GST

Expression

Bacterial

Mutation

amino acids 107-286

Available since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSLI-2xFKBP-GFP(1463000)

Plasmid

#85789

Purpose

P. falciparum integration plasmid to fuse endogenous genes with tags using SLI

Depositor

Tobias Spielmann

Publication

Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

PF3D7146300 targeting region, 2xFKBP-GFP-2TA-Neo-R

Use

P. falciparum integration

Tags

2xFKBP, GFP

Promoter

none

Available since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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