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  1. pETM11-6xHis-TEV-hORF1p_K3AK4A

    Plasmid
    #202585
    Purpose
    Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the K3A and K4A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scar
    Depositor
    Liam Holt
    Article
    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75
    Insert
    ORF1p
    Tags
    6x His and TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    Changed ORF1p Lysine 3 to Alanine, changed ORF1p …
    Available Since
    Nov. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    202585-plasmid-map-sequence-id-414431

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  19. pGEX2TK-Osh2-PH

    Plasmid
    #71370
    Purpose
    bacterial expression of PH domain for phosphoinositide binding assay
    Depositor
    Mark Lemmon
    Article
    Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.
    Insert
    Osh2-PH
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Pleckstrin homology (PH) domain; aa 277-398
    Promoter
    tac
    Available Since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 8981 - 9000 of 61872 results