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pICE TRP1

Plasmid

#122881

Purpose

TRP1 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available Since

Oct. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pICE LYS2

Plasmid

#122879

Purpose

LYS2 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available Since

May 25, 2019

Availability

Academic Institutions and Nonprofits only

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YIp TRP1-KanMx

Plasmid

#122923

Purpose

TRP1-KanMx integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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YIp TRP1-NatMx

Plasmid

#122924

Purpose

TRP1-NatMx integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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pQcre1

Plasmid

#135659

Purpose

Clostridial vector, encoding the Cre protein, which mediates recombination between lox sites.

Depositor

Andrew Tolonen

Article

Cerisy et al mSphere. 2019 Dec 11;4(6). pii: 4/6/e007

Insert

Cre recombinase

Use

Cre/Lox

Expression

Bacterial

Promoter

ppag promoter

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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YIp TRP1-HIS3

Plasmid

#122912

Purpose

TRP1-HIS3 integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available Since

Dec. 3, 2019

Availability

Academic Institutions and Nonprofits only

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YIp ADE2-LEU2

Plasmid

#122905

Purpose

ADE2-LEU2 integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available Since

Oct. 18, 2019

Availability

Academic Institutions and Nonprofits only

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YIp TRP1-HphMx

Plasmid

#122922

Purpose

TRP1-HphMx integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available Since

July 4, 2019

Availability

Academic Institutions and Nonprofits only

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pMKV001

Plasmid

#35107

Depositor

John McCusker

Article

Vorachek-Warren et al Yeast. 2004 Jan 30;21(2):163-71.

Insert

dsdaMX4

Use

Yeast replicating

Available Since

Aug. 29, 2012

Availability

Academic Institutions and Nonprofits only

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lvl0 HSPt

Plasmid

#153378

Purpose

Plant terminator

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

HSP terminator

Expression

Plant

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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lvl0 NDUFA8t

Plasmid

#153379

Purpose

Plant terminator

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

NDUFA8 terminator

Expression

Plant

Available Since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pICE ADE2-HphMx

Plasmid

#122895

Purpose

ADE2-HphMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available Since

Oct. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pET-His-Tre

Plasmid

#123129

Purpose

Bacterial expression plasmid for Tre with an N-terminal His-tag.

Depositor

David Liu

Article

Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

Insert

Tre recombinase

Tags

6x His tag

Expression

Bacterial

Promoter

T7

Available Since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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pGGE009

Plasmid

#48841

Purpose

Provides the UBQ10 terminator as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

UBQ10 terminator

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGR-ECK125108723

Plasmid

#46008

Depositor

Christopher Voigt

Article

Chen et al Nat Methods. 2013 Jun 2. doi: 10.1038/nm

Insert

ECK125108723 Terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

PBAD

Available Since

June 28, 2013

Availability

Academic Institutions and Nonprofits only

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pGR-ECK120010783

Plasmid

#46009

Depositor

Christopher Voigt

Article

Chen et al Nat Methods. 2013 Jun 2. doi: 10.1038/nm

Insert

ECK120010783 Terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

PBAD

Available Since

June 28, 2013

Availability

Academic Institutions and Nonprofits only

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pLIC.B4.Ta1145

Plasmid

#11533

Depositor

Sung-Hou Kim

Article

Shin et al J Biol Chem. 2005 May 6. 280(18):18326-3

Insert

nicotinate phosphoribosyltransferase (Ta1145 T. acidophilum)

Tags

HisTag-MBP, TEV cleavable

Expression

Bacterial

Available Since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
pCMV-T7-ABE8e-SpCas9-NRCH-P2A-EGFP (RMD57)

Plasmid

#197504

Purpose

CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRCH(D10A) and P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

Insert

pCMV-T7-ABE8e-NRCH-P2A-EGFP

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS and BPNLS-P2A-EGFP

Expression

Mammalian

Mutation

ABE8e mutations in TadA and nSpCas9-NRCH(D10A/I32…

Promoter

CMV and T7

Available Since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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pEA01

Plasmid

#170766

Purpose

Utilisation of pSAM2 excision tool in Amycolatopsis spp

Depositor

Jean-Luc Pernodet

Article

Santos et al Microorganisms. 2022 Apr 16;10(4). pii:

Inserts

xis, int
oriT
pac

Expression

Bacterial

Promoter

trcp

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-Halobacterium GvpA scFv 2B10-1-pcDNA 3.4 TOPO

Plasmid

#174688

Purpose

Plasmid for recombinant expression in mammalian cells of an anti-Halobacterium salinarum GvpA single chain variable fragment derived from monoclonal antibody 2B10-1

Depositor

Jean Gariepy

Article

Fernando et al ACS Pharmacol Transl Sci. 2024 Jun 3;7(6

Insert

Anti- Halobacterium salinarum GvpA single chain variable fragment 2B10-1

Tags

hemagglutinin (HA) tag, hexahistidine (HIS) tag

Expression

Mammalian

Mutation

None

Promoter

CMV promoter

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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