We narrowed to 10,128 results for: Met
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Plasmid#236039PurposeThe plasmid pQdCas12a.luxR(mut)-sggfp(A) expresses the dCas12a endonuclease and the sgRNA (design A) targeting the gfp gene. Additionally, it contains a mutation in the luxR gene.DepositorInsertquorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (B) of gfp gene
UseCRISPRPromoterquorum sensing promoterAvailable sinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
MKC84_HBB(HBA1reg-HBA1)
Plasmid#232410PurposeAAV production plasmid for HBA1 UTRs vector from Fig. 3 that mediates HDR at HBB locus using HBB sg7 gRNA. HBA gene includes HBA1 exons and introns; HBA1 UTRs flank HBA1 cassette.DepositorAvailable sinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
MKC83_HBB(HBBreg-HBA1)
Plasmid#232409PurposeAAV production plasmid for HBB UTRs vector from Fig. 3 that mediates HDR at HBB locus using HBB sg7 gRNA. HBA gene includes HBA1 exons and introns; HBB UTRs flank HBA1 cassette.DepositorAvailable sinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
KRD9_HBA1(HBA1reg-HBB)
Plasmid#232402PurposeAAV production plasmid for HBA1 UTRs vector from Fig. 3 that mediates HDR at HBA1 locus using HBA1 sg5 gRNA. HBB gene includes introns; HBA1 UTRs flank HBA1 cassette. HAs are ~400bp each.DepositorAvailable sinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD -3G1S+4V
Plasmid#234612PurposeBacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) with 3 Gly and 1 Ser mutated to AsnDepositorInserthnRNPA1_LCD_4GSV (HNRNPA1 Human)
Tags6xHis-TEVExpressionBacterialMutationG204V, S231V, G274V, G303VPromoterT7Available sinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHD-FlpTag-DsRed-HDR-ph2
Plasmid#231902PurposedsDNA donor vector with 3xP3-DsRed and FlpTag-GFP for precise genomic targeting and integration of the FlpTag-GFP cassette with codon phase 2.DepositorInsertFrt-Frt14-SD-GFP-SA-Frt-Frt14
ExpressionBacterialAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHD-FlpTag-DsRed-HDR-ph0
Plasmid#231900PurposedsDNA donor vector with 3xP3-DsRed and FlpTag-GFP for precise genomic targeting and integration of the FlpTag-GFP cassette with codon phase 0.DepositorInsertFrt-Frt14-SD-GFP-SA-Frt-Frt14
ExpressionBacterialAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD70EN19-Pct5-GPA_25810/25800-(+)-Eldh1/2(GpDSM19378)
Plasmid#225305PurposeExpresses Gordonibacter pamelaeae DSM 19378 (+)-Eldh1 and (+)-Eldh2 in Eggerthella lenta DSM 2243 with a cumate inducible promoterDepositorInsert(+)-Eldh1, (+)-Eldh2
ExpressionBacterialAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
EGFP_EHMT1V09
Plasmid#228813PurposeExpression of mouse EHMT1 with deletion of aa 1-31DepositorAvailable sinceMarch 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pENTR4 HopU1
Plasmid#228395PurposepENTR4 plasmid with CDS of Pseudomonas syringae pv tomato type III effector HopU1 without stop codon for C-terminal epitope tagsDepositorPublicationInsertHopU1
UseGateway CloningMutationAlanine inserted after start Methionine due to Nc…Available sinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
Athal_PheRS_TP_pK7fWG2_IVD_FP611
Plasmid#228370PurposeExpresses N-term transit peptide from Arabidopsis PheRS At3g58140 with demonstrated dual mito/plastid targeting; proof-of-concept for Plasmid 228371DepositorInsertN-terminal transit peptide from Arabidopsis PheRS At3g58140 (Warren et al., 2023) with demonstrated dual mito/plastid targeting.
TagsGreen Florescent ProtienExpressionPlantAvailable sinceFeb. 26, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFastBacI-KDAC8
Plasmid#224343PurposeExpresses human KDAC8 (HDAC8) in insect cellsDepositorAvailable sinceFeb. 26, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMVP-SEC24C-G220C
Plasmid#231560PurposepMVP expression vector for human SEC24C (G220C mutation, closed, resistant to sgSEC24C #1 and #2) (Blasticidin selection marker)DepositorInsertSEC24C (G220C) (SEC24C Human)
UseLentiviralTagsV5ExpressionMammalianMutationG220C, silent PAM mutations (G67 (GGG>GGA) and…PromoterCMVAvailable sinceFeb. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMVP-SEC24C-S107F
Plasmid#231561PurposepMVP expression vector for human SEC24C (S107F mutation, closesd, resistant to sgSEC24C #1 and #2) (Blasticidin selection marker)DepositorInsertSEC24C (S107F) (SEC24C Human)
UseLentiviralTagsV5ExpressionMammalianMutationS107F, silent PAM mutations (G67 (GGG>GGA) and…PromoterCMVAvailable sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGEX-parkin M458L
Plasmid#227356PurposeFor bacterial expression of hyperactive parkin mutant.DepositorInsertParkin M458L (PRKN Human)
TagsGSTExpressionBacterialMutationCodon usage optimized for E.coli expression. Chan…Available sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYX-N-Puro-mNeon-TSC22D1
Plasmid#222667Purposeendogenously tag TSC22D1 with N-terminal mNeonDepositorAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYX-N-Blast-miniTurbo-TSC22D2
Plasmid#222669Purposeendogenously tag TSC22D2 with N-terminal miniTurboDepositorAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pT7-IVT_ACC-msfGFP[r5M]_ACC-mEBFP2
Plasmid#221082PurposePlasmid for producing DNA Templates for in vitro transcription. Produces msfGFP[r5M] and mEBFP2 from IVT mRNA.DepositorInsertACC-msfGFP[r5M]_ACC-mEBFP2
UseSynthetic BiologyPromoterCMV, T7Available sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGECPL.BC10.MG
Plasmid#223822PurposeContains Cas9-editable barcode, marking guide (MG) for lineage tracing, Cre for Cas9 activation and loxP-mediated gene deletion, and Firefly Luciferase (FLuc) for live luminescence-based monitoring.DepositorInsertEf1a-Driven Cre-P2A-FLuc
UseCRISPR, Cre/Lox, Lentiviral, Luciferase, and Mous…TagsP2AExpressionMammalianPromoterEf1aAvailable sinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FKBP-MAVS(trunc Cterm)
Plasmid#221259PurposeExpression of MAVS truncation (393-431) in mammalian cells by retroviral transductionDepositorAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only