We narrowed to 13,392 results for: mpl

1. pD649-HAsp-PTPRD-COMP5AP-AviTag-9xHis

   Plasmid

   #157601

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   PTPRD (PTPRD Human)
   

   Use

   Tags

   COMP5AP-AviTag-9xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/SP6

   Available since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC19-YAP-mEGFP

   Plasmid

   #166472

   Purpose

   HDR template for tagging of endogenous mouse YAP N-terminus with mEGFP

   Depositor

   Shuguo Sun

   Article

   Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

   Insert

   YAP-mEGFP HDR template(mouse Yap) (Yap1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. YAP1-Y357F-V5 in pLX304

   Plasmid

   #42556

   Depositor

   William Hahn

   Article

   Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

   Insert

   YAP1 (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Y357F

   Promoter

   CMV

   Available since

   Feb. 8, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pD649-HAsp-PILRA-Fc(DAPA)-AviTag-6xHis

   Plasmid

   #156577

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   PILRA (PIGR Human)
   

   Use

   Tags

   Fc(DAPA)-AviTag-6xHis

   Expression

   Mammalian

   Mutation

   Fc region of human IgG contains D265A and P329A m…

   Promoter

   CMV/SP6

   Available since

   Aug. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBK1303-AAV-EFSNC-dCjCas9-MECP2(204-310)

   Plasmid

   #223148

   Purpose

   Expression of truncated MECP2 with dCjCas9 and empty gRNA scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   MECP2 (MECP2 Human)
   

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 204-310

   Promoter

   EF1a

   Available since

   Aug. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. Nav1.7 Na+ channel scFv [N68/6]

   Plasmid

   #182094

   Purpose

   Mammalian Expression of Nav1.7 Na+ channel scFv. Derived from hybridoma N68/6.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   recombinant mouse scFv targeting Nav1.7 Na+ channel (Homo sapiens) (SCN9A Mouse)
   

   Use

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIG-703pUC19-tNGFR-P2A-BACH2

   Plasmid

   #186112

   Purpose

   Knockin of truncated NGFR to target gene

   Depositor

   Alexander Marson

   Article

   Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

   Insert

   BACH2 (BACH2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   WT with tNGFR sequence

   Promoter

   Available since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pD649-HAsp-ROR1-Fc(DAPA)-AviTag-6xHis

   Plasmid

   #156810

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   ROR1 (ROR1 Human)
   

   Use

   Tags

   Fc(DAPA)-AviTag-6xHis

   Expression

   Mammalian

   Mutation

   Fc region of human IgG contains D265A and P329A m…

   Promoter

   CMV/SP6

   Available since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pODN-mR2-ACTB

   Plasmid

   #183868

   Purpose

   Repair template for the N-terminal tagging of beta-actin with mRuby2 in human cells using CRISPR/Cas9.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   ACTB homology arms with mRuby2-linker (ACTB Human)
   

   Use

   CRISPR; Donor template

   Tags

   mRuby2-linker

   Expression

   Mammalian

   Mutation

   Homology arms contain point mutations to remove t…

   Promoter

   Available since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMRX-IP-muGFP-hATG2A ΔWIR

    Plasmid

    #215507

    Purpose

    Expresses GFP tagged human ATG2A with the mutation in WIPI interacting region.

    Depositor

    Noboru Mizushima

    Article

    Shimizu et al Hum Mol Genet. 2023 Aug 7;32(16):2623-26

    Insert

    Autophagy related 2A (ATG2A Human)
    

    Use

    Retroviral

    Tags

    muGFP

    Expression

    Mammalian

    Mutation

    P656R (natural variant with no functional relevan…

    Promoter

    Available since

    June 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNTKV- Elf5-KI-3'UTR-2A-EYFP-NLS

    Plasmid

    #128833

    Purpose

    Knock-in of nuclear EYFP into mouse Elf5 locus

    Depositor

    Yossi Buganim

    Article

    Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

    Insert

    Nuclear EYFP into Elf5 locus (Elf5 Mouse)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pD649-HAsp-PTPRS-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #157038

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    PTPRS (PTPRS Human)
    

    Use

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pD649-HAsp-LAIR1-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156536

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    LAIR1 (LAIR1 Human)
    

    Use

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pD649-HAsp-SIGLEC15-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156698

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    SIGLEC15 (SIGLEC15 Human)
    

    Use

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Anti-NALCN [N185/7R]

    Plasmid

    #177508

    Purpose

    Mammalian Expression Plasmid of anti-NALCN (Rat). Derived from hybridoma N185/7.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-NALCN (Rattus norvegicus) recombinant mouse monoclonal antibody (Nalcn Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    Nov. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-hSyn-DIO {hCAR}off-{DTR-GFP}on-W3SL

    Plasmid

    #111396

    Purpose

    AAV vector with hSynapsin promoter, Cre-OFF hCAR (for efficient CAV-2 infection), Cre-ON DTR-EGFP (diphtheria toxin receptor for cell ablation), and W3SL cassette (for maximize cloning capacity)

    Depositor

    Adam Kepecs

    Article

    Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

    Inserts

    hCAR (CXADR Human)
    DTR-EGFP
    

    Use

    AAV

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin

    Available since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pD649-HAsp-MXRA5(Trunc3)-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #157014

    Purpose

    Mammalian expression of cell-surface protein partial extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    MXRA5 (MXRA5 Human)
    

    Use

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pD649-HAsp-TIGIT-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156497

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    TIGIT (TIGIT Human)
    

    Use

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. RP1B FUS 1-163 S12xQ

    Plasmid

    #127193

    Purpose

    Protein expression for affinity purification

    Depositor

    Nicolas Fawzi

    Article

    Murthy et al Nat Struct Mol Biol. 2019 Jul;26(7):637-

    Insert

    FUS 1-163 S12xQ (FUS Human)
    

    Use

    Tags

    hexahistidine

    Expression

    Bacterial

    Mutation

    S12xQ: S30Q, S44Q, S48Q, S53Q, S70Q, S84Q, S89Q, …

    Promoter

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTR-177 pUC19-CLTA-N-mCherry

    Plasmid

    #186128

    Purpose

    knockin of mCherry to CLTA target

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    CLTA (CLTA Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with mCherry sequence

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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