We narrowed to 63,052 results for: cloning
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Plasmid#121764PurposepMVP L3-L2 entry plasmid, contains eGFP-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term eGFP linked by P2A to gene of interest.DepositorInsertP2A-eGFP + polyA
UseGateway Cloning and Synthetic BiologyAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KO801: pMVP (L5-L4) Hygro-P2A
Plasmid#121718PurposepMVP L5-L4 entry plasmid, contains Hygro-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term Hygro selection marker linked by P2A to gene of interest.DepositorInsertHygro-P2A (open)
UseGateway Cloning and Synthetic BiologyAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
II901: pMVP (L5-L4) FKBP DD
Plasmid#121720PurposepMVP L5-L4 entry plasmid, contains FKBP DD for 4-component MultiSite Gateway Pro assembly. Allows fusion of N-term FKBP degron domain (stabilized by SHLD1) to gene of interest.DepositorInsertFKBP Degron Domain (open)
UseGateway Cloning and Synthetic BiologyAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
II801: pMVP (L5-L4) ecDHFR DD
Plasmid#121721PurposepMVP L5-L4 entry plasmid, contains ecDHFR DD for 4-component MultiSite Gateway Pro assembly. Allows fusion of N-term ecDHFR degron domain (stabilized by TMP) to gene of interest.DepositorInsertecDHFR Degron Domain (open)
UseGateway Cloning and Synthetic BiologyAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
8xCTS1_delta-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro_SV40polyA
Plasmid#124540PurposeCloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.DepositorInsertdelta-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro
ExpressionMammalianMutationd11-41, d11-41PromoterMinimal ADEAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
8xCTS1_deltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro_SV40polyA
Plasmid#124538PurposeCloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.DepositorInsertdeltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro
ExpressionMammalianMutationd11-41 C55G, d11-41PromoterMinimal ADEAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
YPRCΔ15Ω1
Plasmid#101710PurposeGoldenBraid vector pDGB2Ω1, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YPRCΔ15 solo LTR lociDepositorTypeEmpty backboneUseYeast expressionAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
BB3aN_14*
Plasmid#98548Purposeempty BB3 for insertion and overexpression of 1 gene in P. pastorisDepositorTypeEmpty backboneExpressionBacterial and YeastAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
BB3rN_AG
Plasmid#98555Purposeempty BB3 for insertion and overexpression of 6 genes in P. pastorisDepositorTypeEmpty backboneExpressionBacterial and YeastAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
BB3rN_AH
Plasmid#98556Purposeempty BB3 for insertion and overexpression of 7 genes in P. pastorisDepositorTypeEmpty backboneExpressionBacterial and YeastAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
BB3aK_14*
Plasmid#98529Purposeempty BB3 for insertion and overexpression of 1 gene in P. pastorisDepositorTypeEmpty backboneExpressionBacterial and YeastAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
BB3aK_AF
Plasmid#98533Purposeempty BB3 for insertion and overexpression of 5 genes in P. pastorisDepositorTypeEmpty backboneExpressionBacterial and YeastAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
BB3aZ_14*
Plasmid#98542Purposeempty BB3 for insertion and overexpression of 1 gene in P. pastorisDepositorTypeEmpty backboneExpressionBacterial and YeastAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3E-TurboID
Plasmid#166570PurposeMultisite Gateway 3' entry clone for fusions onto the N-terminus of TurboID. Parton lab clone JSSDepositorInsertTurboID
UseGateway CloningAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
mPapaya-C1
Plasmid#54524PurposeLocalization: C1 Cloning Vector, Excitation: 530, Emission: 541DepositorTypeEmpty backboneTagsmPapayaExpressionMammalianAvailable sinceJune 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBlue-TAL
Plasmid#49401PurposeThe pBlue-TAL is suitable for TALEN mutagenesis in insects. It is compatible with Golden Gate Cloning Kit. It has Esp3I cleavage sites and a T7 promoter.DepositorTypeEmpty backboneUseTALENExpressionInsectAvailable sinceDec. 19, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Hs.KRAS4b Q61H
Plasmid#83140PurposeGateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), Q61H mutationDepositorAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLKO5.U6.DR130(CasRX)_Empty.EFS.tRFP657
Plasmid#212961PurposeLentiviral plasmid for the cloning of mature gRNAs of the RfxCas13d (CasRx) CRISPR system. As a marker, the plasmid also encodes the tRFP657 fluorescence protein.DepositorInsertDR1_CasRx_backbone
UseCRISPR and LentiviralTagsTagRFP657ExpressionMammalianPromoterU6Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
phU1-PspCas13b-non-targeting (pEZ36)
Plasmid#229053PurposeCloning backbone for PspCas13b crRNA (Human U1 promoter)DepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTHSSe_46
Plasmid#109244PurposepSC101 var4 BbsI cloning vectorDepositorInsertsfGFP
ExpressionBacterialPromoterPJ23105Available sinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only