We narrowed to 31,178 results for: PLE;
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Plasmid#217723PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRE2 (ADGRE2 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-523Available SinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJZC116
Plasmid#62344PurposesgRNA + 2x MS2(wt+f6) with MCP-VP64 effector for mammalian cells, marked by BFPDepositorInsertssgRNA + 2x MS2 (wt+f6) binding module
MCP-VP64
UseLentiviralTagsVP64ExpressionMammalianMutationTargets CXCR4 , sequence: GCAGACGCGAGGAAGGAGGGCGCPromoterCMV and U6Available SinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dCas12a-A3A-Y132D
Plasmid#193641PurposeExpression of base editor dCas12a-A3A-Y132D in mammalian cells for base editingDepositorInsertdLbCas12a-hA3A-Y132D
ExpressionMammalianMutationhAPOBEC3A-Y132DPromoterCMVAvailable SinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSF3-Flag-Cbir-Ago2_Myc-Nbir-GFP
Plasmid#90015PurposeSplit-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Ago2 and N-terminally tagged NBir[2-256]-GFPDepositorInsertsCBirA*-Ago2
NBirA*-GFP
UseRetroviral; Flp/frtTagsFLAG and MycExpressionMammalianPromoterCMVAvailable SinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRE1 CTFdeltaTA-FLAG
Plasmid#217722PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRE1 (EMR1 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-590Available SinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRF4 CTFdeltaTA-FLAG
Plasmid#217730PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRF4 (ADGRF4 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-390Available SinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRF3 CTFdeltaTA-FLAG
Plasmid#217729PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRF3 (ADGRF3 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-758Available SinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSKC12 - DelQ
Plasmid#110493PurposeMonitor the expression of Luciferase driven by a mutated NRAS 5'-UTR.DepositorInsertNRAS 5'- DelQ (bp 30-254) (NRAS Human)
UseLuciferaseTagsFirefly luciferaseMutationDeletion of the first 29 base pairs of the NRAS 5…PromoterT7Available SinceJuly 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP-itis pBE-nt
Plasmid#195343PurposeBase editor plasmid expressing ABE8e-NG-Cas9n under Theophylline Riboswitch and constituitively expressed nonsense non-targeting gRNADepositorInsertsecTadA(8e)-SpCas9-NG
gRNA gtgcacgacgccgtatgcga
UseCRISPRMutationSpCas9n-NG: D10A, L1111R, D1135V, G1218R, E1219F,…PromoterLac and ProCAvailable SinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTU422
Plasmid#178982Purpose13S-S Candidatus “Scalindua brodae” Twin- Strep SUMO tagged RAMP (codon-optimization) enriched with CRISPR array spacer 1 (five times)DepositorInsertsgRAMP
CRISPR array
TagsStrep-Tag II SUMOExpressionBacterialPromoterT7Available SinceFeb. 10, 2022AvailabilityAcademic Institutions and Nonprofits only