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pcDNA3-ApaLID211A-HA

Plasmid

#211459

Purpose

Express mitochondrial localized ApaLI with D211A mutation

Depositor

Agnel Sfeir

Publication

Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ApaLI-D211A

Tags

HA

Expression

Mammalian

Mutation

D211A

Promoter

CMV

Available since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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BA31_pU.CAG.SaCas9-D10A.rBGpA

Plasmid

#199251

Purpose

Expression construct encoding the S. aureus Cas9.D10A nickase (SaCas9.D10A)

Depositor

Manuel Goncalves

Publication

Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SaCas9.D10A nickase

Use

CRISPR

Tags

3XHA epitope, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Mutation

D10A

Promoter

CAG promoter

Available since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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EC71100

Plasmid

#154068

Purpose

Level 0 Golden Gate vector, pHvHSP17

Depositor

Cristobal Uauy

Publication

Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

Insert

pHvHSP17

Use

Standard cloning vector used by geneart for synth…

Mutation

All BsaI, Esp3I and BpiI restriction sites were r…

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pEasyCas9

Plasmid

#184909

Purpose

Expresses the Streptococcus pyogenes Cas9 protein

Depositor

Jeferson Gross

Publication

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

Cas9

Use

CRISPR

Expression

Bacterial and Yeast

Available since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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PDF1::mCitrine-MBD

Plasmid

#115222

Purpose

Microtubule visualization

Depositor

Jan Traas

Publication

Armezzani et al Development. 2018 Jun 4;145(11). pii: de

Insert

Microtubule binding domain

Tags

mCitrine

Expression

Plant

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223-NME6

Plasmid

#23562

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

NME6 (NME6 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pIOM15

Plasmid

#20869

Depositor

Carmen Beuzón

Publication

Ortiz-Martin et al J Microbiol Methods. 2006 Dec . 67(3):39

Insert

aphA::aadA

Expression

Bacterial

Available since

July 15, 2009

Availability

Academic Institutions and Nonprofits only
pET-22b(+)-HpENR

Plasmid

#12651

Depositor

SeWon Suh

Publication

Lee et al Acta Crystallogr D Biol Crystallogr. 200

Insert

noyl-acyl carrier protein reductase (fabI Helicobacter pylori)

Expression

Bacterial

Available since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFastBac1_GI.1_WT

Plasmid

#162579

Purpose

Expresses norovirus GI.1 VP1 protein in Sf9 insect cells

Depositor

Peter Kwong

Publication

Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

Insert

VP1 protein from human norovirus GI.1

Expression

Insect

Promoter

polyhedrin

Available since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pOTTC840 - pAAV 5xATF6 secNLuc

Plasmid

#82497

Purpose

Longitudinal monitoring of Gaussia and Nano luciferase activities to simultaneously assess ER calcium homeostasis and ER stress in vivo

Depositor

Brandon Harvey

Publication

Wires et al PLoS One. 2017 Apr 12;12(4):e0175481. do

Insert

secNLuc

Use

AAV

Expression

Mammalian

Promoter

5x(ATF6 binding site) c-Fos minimal promoter

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pNCST-mAvicFP1

Plasmid

#129509

Purpose

Expresses mAvicFP1 constitutively in E. coli (most strains)

Depositor

Nathan Shaner

Publication

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

mAvicFP1

Expression

Bacterial

Promoter

synthetic constitutive (stationary phase) promote…

Available since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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ERG_pet28a

Plasmid

#131642

Purpose

Bacterial expression of ETS gene ERG

Depositor

Peter Hollenhorst

Publication

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ERG (ERG Human)

Tags

6xHis

Expression

Bacterial

Available since

Oct. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pGL4.13/AUG-FFLuc-3XFLAG

Plasmid

#127333

Purpose

Express 3xFLAG tagged firefly luciferase (FFLuc) from AUG start codon

Depositor

Jeremy Wilusz

Publication

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Firefly luciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CV40

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBF_HsPepT1

Plasmid

#73943

Purpose

Xenopus oocyte expression vector containing the cDNA of human PepT1 with a c terminal flag tag

Depositor

Simon Newstead

Publication

Beale et al Structure. 2015 Oct 6;23(10):1889-99. do

Insert

PepT1 (SLC15A1 Human)

Use

Make rna for xenopus oocyte injection

Tags

Flag

Promoter

beta globin

Available since

April 19, 2016

Availability

Academic Institutions and Nonprofits only
myr-FLAG-PIK3CA-pcw107

Plasmid

#64605

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Publication

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

PIK3CA (PIK3CA Bovine)

Use

Lentiviral

Tags

FLAG and myristoylated

Promoter

PGK

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pSUPER retro puro macroH2A1 shRNA

Plasmid

#30517

Depositor

John Gurdon

Publication

Pasque et al EMBO J. 2011 May 6;30(12):2373-87. doi:

Insert

mH2A1 shRNA

Use

RNAi and Retroviral

Expression

Mammalian

Available since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only

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pSUPER retro puro macroH2A2 shRNA

Plasmid

#30518

Depositor

John Gurdon

Publication

Pasque et al EMBO J. 2011 May 6;30(12):2373-87. doi:

Insert

mH2A2 shRNA

Use

RNAi and Retroviral

Expression

Mammalian

Available since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only

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pGBT-R16

Plasmid

#31827

Depositor

Stephen Ekker

Publication

Clark et al Nat Methods. 2011 Jun;8(6):506-15. Epub

Insert

mini Tol2 ITR

Use

Cre/Lox; Gene trap

Tags

mRFP

Mutation

mRFP lacks start codon

Promoter

beta Actin

Available since

Sept. 15, 2011

Availability

Academic Institutions and Nonprofits only

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pDONR223-ASCIZ

Plasmid

#23571

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ASCIZ (ATMIN Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
HOXC13(human)-pET

Plasmid

#8679

Depositor

Corey Largman

Publication

Komuves et al Dev Dyn 2003 Jun;227(2):192-202.

Insert

HOXC13 (HOXC13 Human)

Tags

His and T7

Expression

Bacterial

Mutation

makes protein by Coomassie blue staining

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only