Addgene

We narrowed to 25,909 results for: Vit;

Showing: 1801 - 1840 of 25909 results

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LSB-hsa-miR-152-3p

Plasmid

#103260

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-152-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-152-3p target (MIR152 Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LSB-hsa-miR-145-5p

Plasmid

#103246

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-145-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-145-5p target (MIR145 Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 2xFLAG-2xSTREP EMI1

Plasmid

#158947

Purpose

Express human EMI1 in mammalian cells

Depositor

Michele Pagano

Article

Marzio et al Mol Cell. 2019 Jan 17;73(2):224-237.e6.

Insert

EMI1 (FBXO5 Human)

Use

Tags

2X FLAG 2X STREP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
LSB-hsa-miR-1908-5p

Plasmid

#103296

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-1908-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-1908-5p target (MIR1908 Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LSB-hsa-miR-181d-5p

Plasmid

#103281

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-181d-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-181d-5p target (MIR181D Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LSB-hsa-miR-99a-3p

Plasmid

#103764

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-99a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-99a-3p target (MIR99A Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LSB-hsa-miR-616-5p

Plasmid

#103677

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-616-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-616-5p target (MIR616 Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LSB-hsa-miR-132-5p

Plasmid

#103220

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-132-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-132-5p target (MIR132 Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LSB-hsa-miR-149-3p

Plasmid

#103254

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-149-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-149-3p target (MIR149 Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LSB-hsa-miR-149-5p

Plasmid

#103255

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-149-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 FPs. . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-149-5p target (MIR149 Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LSB-hsa-miR-140-5p

Plasmid

#103237

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-140-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-140-5p target (MIR140 Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LSB-hsa-miR-182-3p

Plasmid

#103282

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-182-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-182-3p target (MIR182 Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LSB-hsa-miR-150-3p

Plasmid

#103256

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-150-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-150-3p target (MIR150 Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LSB-hsa-miR-126-3p

Plasmid

#103192

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-126-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-126-3p target (MIR126 Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LSB-hsa-miR-22-3p

Plasmid

#103363

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-22-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-22-3p target (MIR22 Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LSB-hsa-miR-125a-3p

Plasmid

#103187

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-125a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-125a-3p target (MIR125A Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
STARR-seq luciferase validation vector_SCP1_CMV

Plasmid

#99314

Purpose

Luciferase validation vector with CMV enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity

Depositor

Alexander Stark

Article

Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

Insert

CMV enhancer

Use

Luciferase; Starr-seq luciferase validation vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
LSB-hsa-miR-302b-5p

Plasmid

#103402

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-302b-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-302b-5p target (MIR302B Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LSB-hsa-miR-24-1-5p

Plasmid

#103374

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-24-1-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-24-1-5p target (MIR24-1 Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LSB-hsa-miR-148b-3p

Plasmid

#103252

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-148b-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-148b-3p target (MIR148B Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LSB-hsa-miR-195-5p

Plasmid

#103312

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-195-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-195-5p target (MIR195 Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LSB-hsa-miR-130a-3p

Plasmid

#103216

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-130a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-130a-3p target (MIR130A Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LSB-hsa-miR-28-3p

Plasmid

#103385

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-28-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-28-3p target (MIR28 Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LSB-hsa-miR-630

Plasmid

#103690

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-630 target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-630 target (MIR630 Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LSB-hsa-miR-633

Plasmid

#103691

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-633 target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-633 target (MIR633 Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LSB-hsa-miR-135b-5p

Plasmid

#103229

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-135b-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-135b-5p target (MIR135B Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LSB-hsa-miR-30a-5p

Plasmid

#103410

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-30a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-30a-5p target (MIR30A Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LSB-hsa-miR-584-5p

Plasmid

#103665

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-584-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-584-5p target (MIR584 Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LSB-hsa-let-7g-5p

Plasmid

#103158

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-let-7g-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-let-7g-5p target (MIRLET7G Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LSB-hsa-miR-125a-5p

Plasmid

#103188

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-125a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-125a-5p target (MIR125A Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LSB-hsa-miR-876-3p

Plasmid

#103742

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-876-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-876-3p target (MIR876 Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LSB-hsa-miR-301a-3p

Plasmid

#103396

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-301a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-301a-3p target (MIR301A Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LSB-hsa-miR-30c-1-3p

Plasmid

#103413

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-30c-1-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-30c-1-3p target (MIR30C1 Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LSB-hsa-miR-25-5p

Plasmid

#103376

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-25-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-25-5p target (MIR25 Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LSB-hsa-let-7e-5p

Plasmid

#103153

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-let-7e-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-let-7e-5p target (MIRLET7E Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LSB-hsa-let-7e-3p

Plasmid

#103152

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-let-7e-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-let-7e-3p target (MIRLET7E Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
STARR-seq luciferase validation vector_mP_CMV

Plasmid

#99313

Purpose

Luciferase validation vector with CMV enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity

Depositor

Alexander Stark

Article

Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

Insert

CMV enhancer

Use

Luciferase; Starr-seq luciferase validation vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCSmChnERT(#99)

Plasmid

#184063

Purpose

cyclofen-inducible mCherry nuclear relocation via mammalian cell transfection or mRNA synthesis

Depositor

Sophie Vriz

Article

Sinha et al Chembiochem. 2010 Mar 22;11(5):653-63. d

Insert

mCherry-nls-ERT2

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

ERT2: G400V, M543A, L544A, deleted 1-281;

Promoter

sCMV+SP6

Available since

Aug. 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-Cerulean3-cpCerulean3-FLARE-AKAR

Plasmid

#123336

Purpose

Cyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity. Contains monomeric Cerulean3/cpCerulean3-E173 homoFRET pair.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

Cerulean3-cpCerulean3-FLARE-AKAR

Use

Tags

6xHIS, Cerulean3, T7 tag (gene 10 leader), Xpress…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
LSB-hsa-miR-27a-5p

Plasmid

#103382

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-27a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-27a-5p target (MIR27A Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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