We narrowed to 27,033 results for: EMB

1. pcDNA-Flag-H3.3-polyA

   Plasmid

   #59780

   Purpose

   Express Flag-tagged H3.3 (H3F3B) in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

   Depositor

   Yi Zhang

   Article

   Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

   Insert

   H3F3B (H3-3B Human)
   

   Expression

   Mammalian

   Promoter

   CMV and T7

   Available Since

   Sept. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-RAM-d2TTA::TRE-FLEX-tdTomato-WPREpA

   Plasmid

   #84468

   Purpose

   pAAV-CRAM-tdTomato

   Depositor

   Yingxi Lin

   Article

   Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

   Insert

   tdTomato
   

   Use

   AAV

   Mutation

   T230I (please see depositor comments below)

   Promoter

   TRE

   Available Since

   Dec. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-RAM-d2TTA::TRE-FLEX-MCS-WPRE-pA

   Plasmid

   #84467

   Purpose

   Empty pAAV-CRAM plasmid w. multiple cloning site (MCS)

   Depositor

   Yingxi Lin

   Article

   Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

   Type

   Empty backbone

   Use

   AAV

   Promoter

   pRAM and TRE

   Available Since

   Jan. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLNCX2-mCherry-CHMP2A

   Plasmid

   #115330

   Purpose

   expresses mCherry-CHMP2A in mammalian cells

   Depositor

   Sanford Simon

   Article

   Johnson et al Elife. 2018 Jul 4;7. pii: 36221. doi: 10

   Insert

   CHMP2A (CHMP2A Human)
   

   Use

   Retroviral

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. 3XFlag pCMV5-TOPO TAZ WT

   Plasmid

   #24809

   Depositor

   Jeff Wrana

   Article

   Varelas et al Nat Cell Biol. 2008 Jul . 10(7):837-48.

   Insert

   TAZ (WWTR1 Human)
   

   Tags

   3XFlag

   Expression

   Mammalian

   Available Since

   May 14, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. GST-Delta23C11orf83

   Plasmid

   #65849

   Purpose

   bacterial expression of GST (N-ter) - Delta 23 C11orf83/UQCC3 (UQCC3 protein depleted of its N-terminal transmembrane domain)

   Depositor

   Amos Bairoch

   Article

   Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d

   Insert

   UQCC3 depleted of its N terminal transmembrane part (UQCC3 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   deletion of the 23 first aa (TM)

   Available Since

   July 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLNCX2-mCherry-CHMP2B

   Plasmid

   #115331

   Purpose

   expresses mCherry-CHMP2B in mammalian cells

   Depositor

   Sanford Simon

   Article

   Johnson et al Elife. 2018 Jul 4;7. pii: 36221. doi: 10

   Insert

   CHMP2B (CHMP2B Human)
   

   Use

   Retroviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   silent mutation in position 4205 C to T

   Promoter

   CMV

   Available Since

   Sept. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-RAM-d2TTA::TRE-ArchT-WPREpA

   Plasmid

   #84472

   Purpose

   pAAV-RAM-ArchT

   Depositor

   Yingxi Lin

   Article

   Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

   Insert

   ArchT
   

   Use

   AAV

   Tags

   EGFP

   Promoter

   TRE

   Available Since

   Jan. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLNCX2-mEGFP-CHMP2A

   Plasmid

   #115328

   Purpose

   expresses mEGFP-CHMP2A in mammalian cells

   Depositor

   Sanford Simon

   Article

   Johnson et al Elife. 2018 Jul 4;7. pii: 36221. doi: 10

   Insert

   CHMP2A (CHMP2A Human)
   

   Use

   Retroviral

   Tags

   mEGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. EYFP-hDF-deltaTM-pCMV-9

    Plasmid

    #72586

    Purpose

    human dysferlin no transmembrane domain tagged with YFP

    Depositor

    Renzhi Han

    Article

    Xu et al PLoS One. 2011;6(11):e27884. doi: 10.137

    Insert

    dysferlin (DYSF Human)
    

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    transmembrane domain deleted

    Promoter

    CMV

    Available Since

    April 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA3_altPKA-Cα-mEGFP

    Plasmid

    #114132

    Purpose

    Alternative spliced variant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric EGFP.

    Depositor

    Haining Zhong

    Article

    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

    Insert

    18747 (Prkaca Mouse)
    

    Expression

    Mammalian

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHis6-hDF-deltaTM-pCMV-9

    Plasmid

    #72587

    Purpose

    human dysferlin no transmembrane domain tagged with his tag

    Depositor

    Renzhi Han

    Article

    Xu et al PLoS One. 2011;6(11):e27884. doi: 10.137

    Insert

    dysferlin (DYSF Human)
    

    Tags

    His

    Expression

    Mammalian

    Mutation

    transmembrane domain deleted

    Promoter

    CMV

    Available Since

    April 15, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pCRE-iRFP670

    Plasmid

    #82696

    Purpose

    Expresses CRE and iRFP670 under two separate PGK promoters.

    Depositor

    Alan Mullen

    Article

    Daneshvar et al Cell Rep. 2016 Oct 4;17(2):353-365. doi:

    Insert

    CRE, iRFP670
    

    Use

    Cre/Lox

    Available Since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pgRGFP

    Plasmid

    #82695

    Purpose

    Expresses gRNA of interest under U6 promoter (cloning by BpiI) and GFP under PGK promoter.

    Depositor

    Alan Mullen

    Article

    Daneshvar et al Cell Rep. 2016 Oct 4;17(2):353-365. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    PGK, U6

    Available Since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX458-sgRNA_Ago1_1

    Plasmid

    #73533

    Purpose

    Expresses SpCas9, GFP, and sgRNA targeting Ago1

    Depositor

    Constance Ciaudo

    Article

    Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

    Insert

    AGO1
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX458-sgRNA_Ago1_2

    Plasmid

    #73534

    Purpose

    Expresses SpCas9, GFP, and sgRNA targeting Ago1

    Depositor

    Constance Ciaudo

    Article

    Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

    Insert

    AGO1
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

17. pBTK614

    Plasmid

    #110608

    Purpose

    BTK assembled plasmid - Encodes dCas9 with no targeting sgRNA (used in Stage 2 assembly to repress target genes)

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    dCas9
    

    Use

    Synthetic Biology

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHisAlix deltaPRD

    Plasmid

    #42577

    Depositor

    James Hurley

    Article

    Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

    Insert

    delta-PRD ALIX (residues 1-702) (PDCD6IP Human)
    

    Tags

    HIS-TEV

    Expression

    Bacterial

    Mutation

    silent mutation A1617G (numbering in ALIX gene)

    Available Since

    Dec. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pX458-sgRNA_Ago2_3

    Plasmid

    #73531

    Purpose

    Expresses SpCas9, GFP, and sgRNA targeting Ago2

    Depositor

    Constance Ciaudo

    Article

    Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

    Insert

    AGO2
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pX458-sgRNA_Ago2_4

    Plasmid

    #73532

    Purpose

    Expresses SpCas9, GFP, and sgRNA targeting Ago2

    Depositor

    Constance Ciaudo

    Article

    Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

    Insert

    AGO2
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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