We narrowed to 4,743 results for: MLS

1. pBS FGF3 isoform 1

   Plasmid

   #19230

   Depositor

   Cliff Tabin

   Article

   Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

   Insert

   Fibroblast growth factor receptor 3 isoform 1 (FGFR3 Chicken)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 19, 2008

   Availability

   Academic Institutions and Nonprofits only

2. pBS ABI3BP

   Plasmid

   #19191

   Depositor

   Cliff Tabin

   Article

   Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

   Insert

   ABI3BP (ABI3BP Chicken)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

3. pBS ADAMTS3

   Plasmid

   #19205

   Depositor

   Cliff Tabin

   Article

   Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

   Insert

   ADAM metallopeptidase with thrombospondin type 1 (ADAMTS3 Chicken)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

4. pRS416 Gal-RNQ1 L94A-YFP

   Plasmid

   #18692

   Depositor

   Susan Lindquist

   Article

   Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

   Insert

   Rnq1 L94A (RNQ1 Budding Yeast)
   

   Use

   Tags

   YFP

   Expression

   Yeast

   Mutation

   Leucine 94 mutated to Alanine (L94A)

   Promoter

   Available since

   June 11, 2008

   Availability

   Academic Institutions and Nonprofits only

5. pLVX-TetOne-Puro-LUC

   Plasmid

   #218630

   Purpose

   This plasmid contains luciferase under doxycycline inducible control.

   Depositor

   Valentin Cracan

   Article

   Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038

   Insert

   LUC (LOC116160065 )
   

   Use

   Lentiviral, Luciferase, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-6xHis-(-30)GFP

   Plasmid

   #62936

   Purpose

   Expression of 6xHis-(-30)GFP in bacterial cells

   Depositor

   David Liu

   Article

   Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

   Insert

   (-30)GFP
   

   Use

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. UAS:mCherry

   Plasmid

   #170994

   Purpose

   UAS:GAL4 repeat-containing promoter drives simple mCherry reporter gene. Blasticidin selectable.

   Depositor

   Alice Ting

   Article

   Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

   Insert

   UAS:mCherry
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Synthetic 14x UAS:Gal4

   Available since

   Aug. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-CAG-FLEX-BFP2-(mCherry)’

   Plasmid

   #115234

   Purpose

   Lentiviral transfer plasmid for expression of mTagBFP2 before Cre recombination and mCherry after it

   Depositor

   Ian Wickersham

   Article

   Jin et al Proc Natl Acad Sci U S A. 2023 Feb 14;12

   Insert

   mTagBFP2 and mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCA24N-p50-ligase

   Plasmid

   #87742

   Purpose

   IPTG-inducible expression of p50-ligase fusion for protein purification

   Depositor

   Wayne Patrick

   Article

   Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

   Insert

   T4 DNA ligase (30 )
   

   Use

   Tags

   6xHis and human p50/NF-kB (amino acids 40-366)

   Expression

   Bacterial

   Mutation

   Promoter

   T5-lac

   Available since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-DIO-EF1α-mKate2-biPAC

    Plasmid

    #169127

    Purpose

    AAV plasmid containing a mKate2 and a light-gated adenylate cyclase biPAC

    Depositor

    Georg Nagel

    Article

    Zhang et al Nature. 2021 Sep;597(7875):245-249. doi:

    Insert

    mKate2-biPAC
    

    Use

    AAV and Cre/Lox

    Tags

    mKate2

    Expression

    Mammalian

    Mutation

    codon optimized to mouse

    Promoter

    EF1a

    Available since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV CMV-dSaCas9-KRAB-bGHpA

    Plasmid

    #106219

    Purpose

    AAV expressing deactivated S. aureus Cas9 fused to a KRAB repressor

    Depositor

    Charles Gersbach

    Article

    Thakore et al Nat Commun. 2018 Apr 26;9(1):1674. doi:

    Insert

    dead S. aureus Cas9 KRAB
    

    Use

    AAV and CRISPR

    Tags

    HA-NLS

    Expression

    Mammalian

    Mutation

    D10A and N580A

    Promoter

    CMV

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-6xHis-(pos36)GFP

    Plasmid

    #62937

    Purpose

    Expression of 6xHis-(+36)GFP in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    (pos36)GFP
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRPN265NB-enh-MNase

    Plasmid

    #166035

    Purpose

    Expresses a nanobody against GFP fused to micrococcal nuclease for purposes of green Cut & Run

    Depositor

    Marc Timmers

    Article

    Nizamuddin et al Nucleic Acids Res. 2021 May 21;49(9):e49

    Insert

    Glutathione-S-Transferase/enhancer/MNase
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    lac promoter / tac promoter

    Available since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV-REPORT(PGKCMV)-nucTomato-hygro

    Plasmid

    #208573

    Purpose

    LV REPORT containing minimal promoter driving monomeric nuclear Tomato followed by an PGK/CMV promoter driving nuclear d2eGFP E2A Hygromycin

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Inserts

    mnucTomato
    d2nucEGFP
    HygroR
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK/CMV and minP

    Available since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCA24N-sso7d-ligase

    Plasmid

    #87745

    Purpose

    IPTG-inducible expression of sso7d-ligase fusion for protein purification

    Depositor

    Wayne Patrick

    Article

    Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

    Insert

    T4 DNA ligase (30 )
    

    Use

    Tags

    6xHis and codon optimized sso7d (Sulfolobus solfa…

    Expression

    Bacterial

    Mutation

    Promoter

    T5-lac

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pETSUMO2_Vitiosangium-bGSDM-thrombin

    Plasmid

    #222099

    Purpose

    For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Vitiosangium species with a thrombin protease cleavage site for controlled activation.

    Depositor

    Philip Kranzusch

    Article

    Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:

    Insert

    Vitiosangium-bGSDM-thrombin ORF
    

    Use

    Tags

    6xHis-SUMO2

    Expression

    Bacterial

    Mutation

    N-terminal methionine deleted and replaced by sin…

    Promoter

    T7

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pETSUMO2_Nostoc-bGSDM-WELQ

    Plasmid

    #222100

    Purpose

    For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Nostoc species with a WELQut protease cleavage site for controlled activation.

    Depositor

    Philip Kranzusch

    Article

    Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:

    Insert

    Nostoc-bGSDM-WELQ ORF
    

    Use

    Tags

    6xHis-SUMO2

    Expression

    Bacterial

    Mutation

    N-terminal methionine deleted and replaced by sin…

    Promoter

    T7

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV-REPORT(PGK/CMV)

    Plasmid

    #172330

    Purpose

    LV REPORT containing minimal promoter driving monomeric nuclear Tomato 2a HSVtk 2a Neo followed by an PGK/CMV promoter driving nuclear d2eGFP E2A Hygromycin

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Inserts

    monomeric nuclear Tomato
    d2eGFP
    HygR
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3x SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    PGK/CMV

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. mCerulean3-Rap1

    Plasmid

    #134928

    Purpose

    Fluorescently tagged GTPase.

    Depositor

    Klaus Hahn

    Article

    O'Shaughnessy et al J Cell Biol. 2019 Sep 2;218(9):3153-3160

    Insert

    Rap1B, Cerulean
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMCB619

    Plasmid

    #171011

    Purpose

    CRISPR sgRNA vector (puro-BFP with BstXI/BlpI digest sites).

    Depositor

    Alice Ting

    Article

    Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

    Insert

    U6:sgRNA_Puro-T2A-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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