-
Plasmid#202178PurposeLevel 0 plasmid containing a terminator sequence from gene 34971 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.DepositorInsertH4 terminator
UseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 28, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pL0_EF-fcpA
Plasmid#202164PurposeLevel 0 plasmid containing a terminator sequence from gene 18049 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.DepositorInsertfcpA terminator
UseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 28, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pL0_AC-H4
Plasmid#202122PurposeLevel 0 plasmid containing the promoter from gene 34971 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.DepositorInsertH4 promoter
UseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 24, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pL0_AC-P49
Plasmid#202120PurposeLevel 0 plasmid containing the promoter from gene 49202 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.DepositorInsertP49 promoter
UseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 24, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET51b(+)-NADPH-LUCID-1 (C50 5.1 nM)
Plasmid#117908PurposePlasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 5.1 nMDepositorInsertSNAP_Pro30_[cpNLuc23_24]eDHFR
UseTagsHis-tag and Strep-tagExpressionBacterialMutationPromoterT7Available sinceOct. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFIN-IRBP1783-CHER
Plasmid#44357DepositorInsertIRBP1783-CHER
UseLentiviralTagsExpressionMutationPromotermurine interphotoreceptor retinoid-binding proteiā¦Available sinceMay 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
delta-EGFP
Plasmid#119223PurposeGABAA receptor expression (delta subunit with EGFP tag at the C-terminus)DepositorInsertGABRD (Gabrd Rat)
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSYN-VWF-039
Plasmid#120292PurposeVWF expression construct expressing D'D3 domains with C1099A/C1142A mutations in D3 domain as an Fc fusion proteinDepositorInsertVWF D1-D2-D'-D3-Fc
UseTagsFcExpressionMammalianMutationC1099A/C1142APromoterCMVAvailable sinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)-NADPH-LUCID-4 (C50 6.5 uM)
Plasmid#117911PurposePlasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 6.5 uMDepositorInsertSNAP_Pro30_[cpNLuc23_24]eDHFR(R44A-H45Q)
UseTagsHis-tag and Strep-tagExpressionBacterialMutationR44A & H45Q on eDHFRPromoterT7Available sinceOct. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
gamma2 CR shRNA (shRNA2)
Plasmid#120794PurposeshRNA targeting the coding region of the gamma2 mRNADepositorInsertGABRG2 shRNA (Gabrg2 Rat)
UseRNAiTagsExpressionMutationPromoterAvailable sinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
JM3 [JBEI-6891]
Plasmid#89788PurposeBroad host range vector pBBR1-MCS2 containing 'tesA (E. coli), fadB (E. coli), Mlut_11700 (M. luteus) and fadM (E. coli) under control of BAD promoterDepositorInserttesA (E. coli), fadB (E. coli), Mlut_11700 (M. luteus) and fadM (E. coli)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
SPOP-V5 (BACK))
Plasmid#169722PurposeMammalian Expression of mouse SPOP Y353EDepositorInsertSPOP (Spop Mouse)
UseTagsV5ExpressionMammalianMutationY353EPromoterCMVAvailable sinceJune 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)-NADPH-LUCID-3 (C50 1.4 uM)
Plasmid#117910PurposePlasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 1.4 uMDepositorInsertSNAP_Pro30_[cpNLuc23_24]eDHFR(R44A)
UseTagsHis-tag and Strep-tagExpressionBacterialMutationR44A on eDHFRPromoterT7Available sinceOct. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)-NADPH-LUCID-2 (C50 29 nM)
Plasmid#117909PurposePlasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 29 nMDepositorInsertSNAP_Pro30_[cpNLuc23_24]eDHFR(R98A)
UseTagsHis-tag and Strep-tagExpressionBacterialMutationR98A on eDHFRPromoterT7Available sinceOct. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAPi
Plasmid#127548PurposeEmpty control for pGAPi-based RNAi silencing in tandem with APT transcript segmentDepositorInsertAdenine Phosphorybosyltransferase transcript segment
UseRNAi; Empty controlTagsExpressionMutationPromoterUbiquitinAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
MyoUTipGAPi
Plasmid#127549PurposePositive RNAi control for pGAPi-based RNAi silencing in tandem with APT transcript segmentDepositorInsertMyosin XIa+b 5'UTR and Adenine Phosphorybosyltransferase transcript segment
UseRNAi; Positive controlTagsExpressionMutationPromoterUbiquitinAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJL1-aadA
Plasmid#117050PurposeIn vitro expression of aadA (streptomycin resistance gene) from the T7 promoterDepositorInsertaadA
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFIN-IRBP156-tdTOM
Plasmid#44356DepositorInsertIRBP156-tdTOM
UseLentiviralTagsExpressionMutationPromotermurine interphotoreceptor retinoid-binding proteiā¦Available sinceMay 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
-