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p2703-AGER-gRNA2

Plasmid

#216472

Purpose

to express Cas9 from S. pyogenes with 2A-eGFP and sgRNA targeting human AGER endogenous ATG start site

Depositor

Darrell Kotton

Article

Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

Insert

sgRNA targeting human AGER locus

Use

CRISPR

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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p2704-AGER-gRNA3

Plasmid

#216473

Purpose

to express Cas9 from S. pyogenes with 2A-eGFP and sgRNA targeting human AGER endogenous ATG start site

Depositor

Darrell Kotton

Article

Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

Insert

sgRNA targeting human AGER locus

Use

CRISPR

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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p2705-AGER-eGFP

Plasmid

#216474

Purpose

donor vector for targeting an eGFP reporter to the human AGER locus at the endogenous ATG start site

Depositor

Darrell Kotton

Article

Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

Insert

eGFP

Use

CRISPR

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-Hygro-2A-TMRPSS2

Plasmid

#170390

Purpose

Generation of stable lines to enhance SARS-CoV-2 infectivity in ACE2 expressing cells

Depositor

Nir Hacohen

Article

Filbin et al Cell Rep Med. 2021 May 18;2(5):100287. d

Insert

TMPRSS2 (TMPRSS2 Human)

Use

Lentiviral

Promoter

SFFV

Available Since

June 23, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pD649-HAsp-TNFSF12-COMP5AP-AviTag-9xHis

Plasmid

#157187

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

TNFSF12 (TNFSF12 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+/-FLI1-C‐(K)‐DYK

Plasmid

#129497

Purpose

Mammalian expression of human FLI1

Depositor

Marjorie Brand

Article

Palii et al Cell Stem Cell. 2019 May 2;24(5):812-820

Insert

FLI1 (FLI1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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p2709-pHAGE2-Ef1aL-YAP5SA-UBC-tagBFP-loxP

Plasmid

#216478

Purpose

lentiviral dual expression of YAP5SA and tagBFP with loxP site for Cre excision

Depositor

Darrell Kotton

Article

Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

Inserts

YAP5SA
tagBFP

Use

Cre/Lox and Lentiviral

Mutation

S61A, S109A, S127A, S164A, S381A

Promoter

EF1aL and UBC

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-KIR2DL5A-COMP5AP-AviTag-9xHis

Plasmid

#157207

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

KIR2DL5A (KIR2DL5A Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-PILRA-COMP5AP-AviTag-9xHis

Plasmid

#157142

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

PILRA (PILRA Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-KIR2DL5B-COMP5AP-AviTag-9xHis

Plasmid

#157208

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

KIR2DL5B (KIR2DL5B Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-SEMA5A-COMP5AP-AviTag-9xHis

Plasmid

#157572

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

SEMA5A (SEMA5A Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-CEACAM5-COMP5AP-AviTag-9xHis

Plasmid

#157501

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

CEACAM5 (CEACAM5 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-CEACAM6-COMP5AP-AviTag-9xHis

Plasmid

#157287

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

CEACAM6 (CEACAM6 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-HLA-E-COMP5AP-AviTag-9xHis

Plasmid

#157396

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

HLA-E (HLA-E Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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p1303 DV-SFTPC-tdTomato

Plasmid

#126696

Purpose

donor vector for targeting a tdTomato reporter to the human SFTPC locus at the endogenous ATG start site

Depositor

Darrell Kotton

Article

Jacob et al Cell Stem Cell. 2017 Oct 5;21(4):472-488

Insert

tdTomato

Expression

Mammalian

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-flag-hKDM4A-polyA

Plasmid

#101051

Purpose

in vitro transcription (T7 promoter)

Depositor

Yi Zhang

Article

Chung et al Cell Stem Cell. 2015 Dec 3;17(6):758-66.

Insert

KDM4A (KDM4A Human)

Tags

Flag

Expression

Mammalian

Promoter

T7

Available Since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-SIGLEC8-COMP5AP-AviTag-9xHis

Plasmid

#157356

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

SIGLEC8 (SIGLEC8 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-SIGLEC15-COMP5AP-AviTag-9xHis

Plasmid

#157262

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

SIGLEC15 (SIGLEC15 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-tetO-Esrrb

Plasmid

#71152

Purpose

Lentiviral plasmid for tetracycline/doxycycline dependent expression of murine Esrrb

Depositor

Hubert Schorle

Article

Kubaczka et al Cell Stem Cell. 2015 Sep 22. pii: S1934-

Insert

Estrogen related receptor, beta (Esrrb Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

tetO

Available Since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pScalps_Puro_mTet2 catalytic domain

Plasmid

#79554

Purpose

Expression of catalytic domain of mouse Tet2

Depositor

Silvia Monticelli

Article

Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

Insert

Tet2 (Tet2 Mouse)

Use

Lentiviral

Tags

Myc

Mutation

aa990-1912

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only