We narrowed to 1,069 results for: gibson assembly

1. pUC19-Q03JI6

   Plasmid

   #103123

   Purpose

   Cas9 coding gene template with optimized sequence for human codon usage

   Depositor

   Duhee Bang

   Article

   Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

   Insert

   Q03JI6 (Cas9 coding gene from Campylobacter jejuni)
   

   Use

   CRISPR; Cloning vector

   Tags

   SV40NLS

   Expression

   Mutation

   human codon-optimized

   Promoter

   Available since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. PMXS-SLC1A3

   Plasmid

   #72873

   Purpose

   The retroviral SLC1A3 vector was generated by cloning an human SLC1A3 PCR fragment (https://www.addgene.org/32813/)into the pMXS-ires-blast vector by Gibson Assembly

   Depositor

   David Sabatini

   Article

   Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10

   Insert

   SLC1A3 (SLC1A3 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pCMU-4

   Plasmid

   #172353

   Purpose

   CRISPR gene editing in Streptomyces, codon-optimized Cas9 behind theophylline riboswitch and D4 promoter, with custom gRNA

   Depositor

   Eriko Takano

   Article

   Ye et al J Ind Microbiol Biotechnol. 2020 May;47(

   Insert

   D4-TheoR-cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   D4

   Available since

   Dec. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pZH514

   Plasmid

   #102667

   Purpose

   Constitutive expression of GFPmut2; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

   Depositor

   Zach Hensel

   Article

   Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

   Insert

   GFPmut2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pZH516

   Plasmid

   #102669

   Purpose

   Constitutive expression of GFPmut2; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

   Depositor

   Zach Hensel

   Article

   Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

   Insert

   GFPmut2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pZH520

   Plasmid

   #102673

   Purpose

   Tetracycline inducible expression of GFPmut2 with constitutive TetR; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

   Depositor

   Zach Hensel

   Article

   Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

   Insert

   GFPmut2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pZH521

   Plasmid

   #102674

   Purpose

   Tetracycline inducible expression of GFPmut2 with constitutive TetR; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

   Depositor

   Zach Hensel

   Article

   Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

   Insert

   GFPmut2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pZH517

   Plasmid

   #102670

   Purpose

   Tetracycline inducible expression of GFPmut2 using autoregulation; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

   Depositor

   Zach Hensel

   Article

   Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

   Insert

   GFPmut2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pZH519

   Plasmid

   #102672

   Purpose

   Tetracycline inducible expression of GFPmut2 using autoregulation; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

   Depositor

   Zach Hensel

   Article

   Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

   Insert

   GFPmut2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pZH522

    Plasmid

    #102675

    Purpose

    Tetracycline inducible expression of GFPmut2 with constitutive TetR; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

    Depositor

    Zach Hensel

    Article

    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

    Insert

    GFPmut2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pZH518

    Plasmid

    #102671

    Purpose

    Tetracycline inducible expression of GFPmut2 using autoregulation; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

    Depositor

    Zach Hensel

    Article

    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

    Insert

    GFPmut2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pNA-011

    Plasmid

    #238994

    Purpose

    Empty Node A that can be used for the assembly of pEND-11Y

    Depositor

    Javier Santos-Moreno

    Article

    Nevot et al ACS Synth Biol. 2025 Aug 15;14(8):2878-2

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pNC-011

    Plasmid

    #238997

    Purpose

    Empty Node C that can be used for the assembly of pEND-Y11 and pEND-Z11

    Depositor

    Javier Santos-Moreno

    Article

    Nevot et al ACS Synth Biol. 2025 Aug 15;14(8):2878-2

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    July 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHRSV_CHARMS-3xAla

    Plasmid

    #233921

    Purpose

    Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-3xAla (mutated TRAF6 binding site)

    Depositor

    Marcus Taylor

    Article

    Lichtenstein et al bioRxiv 2024.05.08.593169

    Insert

    CHARMS-3xAla
    

    Use

    Lentiviral

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHRSV_CHARMS-DHF

    Plasmid

    #233922

    Purpose

    Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-DHF (Designed Helical Filament)

    Depositor

    Marcus Taylor

    Article

    Lichtenstein et al bioRxiv 2024.05.08.593169

    Insert

    CHARMS-DHF
    

    Use

    Lentiviral

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHRSV_CHARMS_bDD

    Plasmid

    #233931

    Purpose

    Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-dDD contains the bacterial death domain from Nostoc.

    Depositor

    Marcus Taylor

    Article

    Lichtenstein et al bioRxiv 2024.05.08.593169

    Insert

    CHARMS-bDD
    

    Use

    Lentiviral

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHRSV_CHARMS-TIR

    Plasmid

    #233919

    Purpose

    Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS): CHARMS-TIR

    Depositor

    Marcus Taylor

    Article

    Lichtenstein et al bioRxiv 2024.05.08.593169

    Insert

    CHARMS-TIR
    

    Use

    Lentiviral

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pENTR_HA-spGFP11

    Plasmid

    #240224

    Purpose

    Gateway entry clone for cloning in insert sequences by gibson assembly to create a C-terminal HA-spGFP11 tag. No ATG start codon.

    Depositor

    Norbert Perrimon, Justin Bosch

    Article

    Bosch et al bioRxiv 2025.07.09.659702; doi: https://

    Type

    Empty backbone

    Use

    Gateway shuttle vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TRE-mClover3-TDP-43ΔNLS

    Plasmid

    #214917

    Purpose

    inducible expression of TDP-43 harboring mutant NLS and N-terminal mClover3. Expression yields cytosolic TDP-43 that forms peinuclear puncta

    Depositor

    Ophir Shalem

    Article

    Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

    Insert

    TDP-43ΔNLS (TARDBP Human)
    

    Use

    Lentiviral

    Tags

    mClover3

    Expression

    Mutation

    mutant NLS GCAGCAGCAATGGATGAGACAGATGCTTCATCAGCAGT…

    Promoter

    Available since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFP.E222

    Plasmid

    #138248

    Purpose

    Three input / three output logic circuit plasmid - Expresses different intein split ECFs from different inducible promoters

    Depositor

    Baojun Wang

    Article

    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

    Inserts

    araC (araC )
    ECF20.N.(VDA).M86.N2
    Terminator
    SspGyrB.C2.(SAK).ECF16.C
    Terminator
    rhaS
    ECF17.N.(NPC).NrdJ-1.N2
    Terminator
    M86.C2.(SDL).ECF20.C
    Terminator
    luxR
    Terminator
    ECF16.N.(AGG).SspGyrB.N2
    Terminator
    NrdJ-1.C2.(SEI).ECF17.C
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23115, P(araBAD), P(lux2), P(rhaBAD), iJ23101, a…

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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