We narrowed to 1,086 results for: gibson assembly

1. pcDNA5/FRT/TO - RBM28 E8 G>T minigene

   Plasmid

   #169272

   Purpose

   Mammalian vector for constitutive expression of RBM28 Exon 8 G>T minigene

   Depositor

   Susan Baserga

   Article

   Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

   Insert

   RBM28 E8 G>T minigene (RBM28 Human)
   

   Expression

   Mammalian

   Mutation

   NM_018077.2:c.(946G>T)

   Promoter

   CMV

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5/FRT/TO - RBM28 Exon1-6 E5 WT minigene

   Plasmid

   #169273

   Purpose

   Mammalian vector for constitutive expression of RBM28 Exon 1-6 WT minigene

   Depositor

   Susan Baserga

   Article

   Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

   Insert

   RBM28 Exon1-6 WT minigene (RBM28 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5/FRT/TO - RBM28 Exon1-6 E5 GT>A minigene

   Plasmid

   #169274

   Purpose

   Mammalian vector for constitutive expression of RBM28 Exon 1-6 GT>A minigene

   Depositor

   Susan Baserga

   Article

   Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

   Insert

   RBM28 Exon1-6 GT>A minigene (RBM28 Human)
   

   Expression

   Mammalian

   Mutation

   NM_018077.2:c.(541+1_541+2delinsA)

   Promoter

   CMV

   Availability

   Academic Institutions and Nonprofits only

   

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4. plentiCRISPR-sgMDH1

   Plasmid

   #72875

   Purpose

   The lentiviral sgMDH1 vectors was generated via ligation of hybridized oligos (below) into lentiCRISPR-v1 vector linearized with BsmBI using Gibson assembly (NEB

   Depositor

   David Sabatini

   Article

   Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10

   Insert

   sgMDH1
   

   Use

   Lentiviral

   Available Since

   March 4, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pOSV801

   Plasmid

   #126044

   Purpose

   modular integrative vector for the assembly of DNA fragments and expression in Streptomyces species

   Depositor

   Jean-Luc Pernodet

   Article

   Aubry et al Appl Environ Microbiol. 2019 Jun 7. pii:

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. LP102: pMAGIC (R4-R3) NLS-Sp Cas9-NLS

   Plasmid

   #132927

   Purpose

   pMAGIC R4-R3 entry plasmid, contains 2xNLS SpCas9 (nuclease active) for 3 or 4-component MultiSite Gateway Pro assembly.

   Depositor

   Christopher Newgard

   Insert

   humanized SpCas9
   

   Use

   CRISPR and Synthetic Biology; Pmagic gateway entr…

   Available Since

   Oct. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHRSV_CHARMS

   Plasmid

   #233920

   Purpose

   Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS

   Depositor

   Marcus Taylor

   Article

   Lichtenstein et al bioRxiv 2024.05.08.593169

   Insert

   CHARMS
   

   Use

   Lentiviral

   Tags

   mEGFP

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   April 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONOR 6colors

   Plasmid

   #229974

   Purpose

   bigMamAct assembled plasmid for 6colors cellular labelling

   Depositor

   Martin Pelosse

   Article

   Pelosse et al Synthetic Biology 2025.02.13

   Insert

   mito-mCherry, EYFP-tubulin, H2B iRFP720, GTS-mTagBFP, mTFP1-actin
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   See Depositor Comments

   Available Since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMMK ENTR 4 CMV GST mTagBFP

   Plasmid

   #206259

   Purpose

   ENTR Vector 4 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes mTagBFP localised to Golgi organelles under the control of CMV promoter.

   Depositor

   Imre Berger

   Article

   Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

   Insert

   GTS mTagBFP
   

   Use

   Multimate/gateway entr 4

   Tags

   BFGT1 (Golgi localisation peptide)

   Expression

   Mammalian

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMMACE DEST CMV H2B iRFP

    Plasmid

    #206253

    Purpose

    DEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes H2B iRFP713 under the control of CMV promoter. CRE-Acceptor in MultiBac system.

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    H2B iRFP713
    

    Use

    Recombinant baculovirus production, multimate/gat…

    Tags

    iRFP713

    Expression

    Mammalian

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMMACE DEST CMV SpCas9

    Plasmid

    #206268

    Purpose

    DEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes SpCas9 under the control of CMV promoter. CRE-Acceptor in MultiBac system.

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    SpCas9
    

    Use

    CRISPR; Recombinant baculovirus production

    Expression

    Mammalian

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. EF1a-SRRD-V5-APEX2 (in pLEX_307)

    Plasmid

    #214921

    Purpose

    constitutive expression of SRRD fused to V5 and APEX2 - used for APEX2 proximity biotinylation

    Depositor

    Ophir Shalem

    Article

    Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

    Insert

    SRRD (SRRD Human)
    

    Use

    Lentiviral

    Tags

    V5-APEX2

    Available Since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMMACE DEST CMV PE2

    Plasmid

    #206276

    Purpose

    DEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes PE2 under the control of CMV promoter. CRE-Acceptor in MultiBac system.

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    PE2
    

    Use

    CRISPR; Recombinant baculovirus production, multi…

    Expression

    Mammalian

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC19-Q03JI6

    Plasmid

    #103123

    Purpose

    Cas9 coding gene template with optimized sequence for human codon usage

    Depositor

    Duhee Bang

    Article

    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

    Insert

    Q03JI6 (Cas9 coding gene from Campylobacter jejuni)
    

    Use

    CRISPR; Cloning vector

    Tags

    SV40NLS

    Mutation

    human codon-optimized

    Available Since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. PMXS-SLC1A3

    Plasmid

    #72873

    Purpose

    The retroviral SLC1A3 vector was generated by cloning an human SLC1A3 PCR fragment (https://www.addgene.org/32813/)into the pMXS-ires-blast vector by Gibson Assembly

    Depositor

    David Sabatini

    Article

    Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10

    Insert

    SLC1A3 (SLC1A3 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pCMU-4

    Plasmid

    #172353

    Purpose

    CRISPR gene editing in Streptomyces, codon-optimized Cas9 behind theophylline riboswitch and D4 promoter, with custom gRNA

    Depositor

    Eriko Takano

    Article

    Ye et al J Ind Microbiol Biotechnol. 2020 May;47(

    Insert

    D4-TheoR-cas9
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    D4

    Available Since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pZH514

    Plasmid

    #102667

    Purpose

    Constitutive expression of GFPmut2; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

    Depositor

    Zach Hensel

    Article

    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

    Insert

    GFPmut2
    

    Expression

    Bacterial

    Available Since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pZH516

    Plasmid

    #102669

    Purpose

    Constitutive expression of GFPmut2; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

    Depositor

    Zach Hensel

    Article

    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

    Insert

    GFPmut2
    

    Expression

    Bacterial

    Available Since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pZH520

    Plasmid

    #102673

    Purpose

    Tetracycline inducible expression of GFPmut2 with constitutive TetR; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

    Depositor

    Zach Hensel

    Article

    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

    Insert

    GFPmut2
    

    Expression

    Bacterial

    Available Since

    Dec. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pZH521

    Plasmid

    #102674

    Purpose

    Tetracycline inducible expression of GFPmut2 with constitutive TetR; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

    Depositor

    Zach Hensel

    Article

    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

    Insert

    GFPmut2
    

    Expression

    Bacterial

    Available Since

    Dec. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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