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Plasmid#196337PurposeEasy-MISE toolkit Level 1 destination plasmid for cassette cloning with BsaI-based Golden Gate Assembly; allowing fast postitive clones test with mRFP1 fluorescent protein-based red/white screeningDepositorTypeEmpty backboneUseSynthetic Biology; Golden gate assembly backboneTagsExpressionMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only
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pAV10
Plasmid#63213PurposepUC19 backbone (Amp), contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. NotI or FseI sites for TU digest.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pENTR_AtU6p_BsaI_ccdb_gRNA
Plasmid#225986PurposeTo make Gateway entry clone of CRISPR guide RNA under AtU6pDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceOct. 7, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBD016
Plasmid#201307PurposeSr27 as Golden Gate compatible MoClo CDS partDepositorInsertSr27
UseTagsExpressionPlantMutationremoval of BsaI and BpiI sitesPromoterAvailable sinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPD1159 pLink 9
Plasmid#139247PurposeVector that holds the linker for circuits with 9 TUsDepositorInsertLinker9
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD1158 pLink 8
Plasmid#139246PurposeVector that holds the linker for circuits with 8 TUsDepositorInsertLinker8
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD1157 pLink 7
Plasmid#139245PurposeVector that holds the linker for circuits with 7 TUsDepositorInsertLinker7
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD1156 pLink 6
Plasmid#139244PurposeVector that holds the linker for circuits with 6 TUsDepositorInsertLinker6
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD1155 pLink 5
Plasmid#139243PurposeVector that holds the linker for circuits with 5 TUsDepositorInsertLinker5
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD1154 pLink 4
Plasmid#139242PurposeVector that holds the linker for circuits with 4 TUsDepositorInsertLinker4
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD1153 pLink 3
Plasmid#139241PurposeVector that holds the linker for circuits with 3 TUsDepositorInsertLinker3
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD1152 pLink 2
Plasmid#139240PurposeVector that holds the linker for circuits with 2 TUsDepositorInsertLinker2
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD1151 pLink 1
Plasmid#139239PurposeVector that holds the linker for circuits with 1 TUsDepositorInsertLinker1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
C9m
Plasmid#120958PurposeMoClo golden gate assembly CD part for deCFP (cyan fluorescent protein) - Please see Supplemental Documents for annotated Genbank fileDepositorInsertdeCFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C76m
Plasmid#120988PurposeMoClo golden gate assembly CD part for KanR (kanamycin resistance protein). Please see Supplemental Documents for annotated Genbank file.DepositorInsertKanR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAIP4_1EDG
Plasmid#234144PurposeHeterologous protein expression of GUNA_RUMCH in Escherichia coliDepositorInsert1EDG
UseTags10x HisTag-Smt3ExpressionBacterialMutationPromoterT7Available sinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
U4m
Plasmid#121017PurposeMoClo golden gate assembly BC part for UTR1 (very strong RBS). Please see Supplemental Documents for annotated Genbank file.DepositorInsertUTR1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV10.KH
Plasmid#63208PurposepCACS backbone (Amp), Hyg, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEN-X-hPGK-PuroR-Z
Plasmid#218904Purposegolden gate cloningDepositorInserthPGK-PuroR
UseTagsExpressionMammalianMutation246T>A and 398T>CPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAV10.KN
Plasmid#63209PurposepCACS backbone (Amp), cloNAT, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only