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pET21-pelB-LaRIgG-2

Plasmid

#228996

Purpose

For bacterial expression of anti-rabbit IgG nanobody LaRIgG-2, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaRIgG-2

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaGIgG-12

Plasmid

#228999

Purpose

For bacterial expression of anti-mouse IgG nanobody LaMIgG-12, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaGIgG-12

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaRIgG-7

Plasmid

#228997

Purpose

For bacterial expression of anti-rabbit IgG nanobody LaRIgG-7, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaRIgG-7

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaRIgG-1

Plasmid

#228995

Purpose

For bacterial expression of anti-rabbit IgG nanobody LaRIgG-1, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaRIgG-1

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaMIgG-5

Plasmid

#228991

Purpose

For bacterial expression of anti-mouse IgG nanobody LaMIgG-5, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaMIgG-5

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-shNC-mCherry

Plasmid

#222962

Purpose

Negative control for lentiviral shRNA

Depositor

Xinyu Zhao

Article

Guo et al Nat Commun. 2023 Jun 26;14(1):3801. doi:

Insert

Negative control

Use

Lentiviral

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-shFMR1-mCherry

Plasmid

#222963

Purpose

Make lentivirus to knock down human FMR1 gene

Depositor

Xinyu Zhao

Article

Guo et al Nat Commun. 2023 Jun 26;14(1):3801. doi:

Insert

FMR1 shRNA (FMR1 Human)

Use

Lentiviral

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pBMN DHFR-HiBiT / Lyt2

Plasmid

#179731

Purpose

Expresses human dihydrofolate reductase (DHFR) with C-terminal HiBiT tag in mammalian cells. This retroviral vector has an IRES-Lyt-2 downstream of the cloning sites

Depositor

James Inglese

Article

Rana et al Cell Chem Biol. 2024 Feb 15;31(2):221-23

Insert

human DHFR-HiBiT and IRES-Lyt-2 (DHFR Human, Mouse)

Use

Retroviral

Tags

HiBiT

Promoter

LTR/MMLV

Available Since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-3*HA-Palmd

Plasmid

#216833

Purpose

expressing HA tag and the coding sequence of mouse Palmd

Depositor

Yuxuan Guo

Article

Guo et al Acta Pharmacol Sin. 2024 Jul 23. doi: 10

Insert

Palmd (mus musculus) (Palmd Mouse)

Tags

HA

Expression

Mammalian

Available Since

Aug. 8, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-haA3A-CBE-G-N

Plasmid

#220898

Purpose

AAV genome encoding the N-terminal of haA3A-CBE-G

Depositor

Dali Li

Article

Yang et al Nat Chem Biol. 2024 Mar 29. doi: 10.1038

Insert

haA3A-CBE-G-N

Use

AAV

Expression

Mammalian

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-haA3A-CBE-A

Plasmid

#220892

Purpose

Express haA3A-CBE-A (with SpCas9) in mammalian cells

Depositor

Dali Li

Article

Yang et al Nat Chem Biol. 2024 Mar 29. doi: 10.1038

Insert

haA3A-CBE-A

Expression

Mammalian

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-haA3A-CBE-VA-NG

Plasmid

#220896

Purpose

Express haA3A-CBE-VA-NG (with SpCas9-NG variant) in mammalian cells

Depositor

Dali Li

Article

Yang et al Nat Chem Biol. 2024 Mar 29. doi: 10.1038

Insert

haA3A-CBE-VA-NG

Expression

Mammalian

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pNK7187

Plasmid

#219761

Purpose

MoClo-compatible Level 1 for expression PpASCL in plants (N.benthamiana, BY2 cells)

Depositor

Karen Sarkisyan

Article

Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

Insert

PpASCL

Expression

Plant

Promoter

p35s_0.4kb - 5'UTR TMV omega

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-U6-sgRNA-Scaffold-cTNT-SaCas9-HA-OLLAS

Plasmid

#209781

Purpose

Expresses SaCas9 by the specific cTNT promoter and sgRNA scaffold by U6 promoter

Depositor

Yuxuan Guo

Article

Yang et al Circulation. 2024 May 28;149(22):1778-17

Insert

cTNT

Use

AAV

Tags

HA and OLLAS

Promoter

cTNT

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-U6-msCAMK2d-sgRNA-cTNT-SaCas9-HA-OLLAS

Plasmid

#209782

Purpose

Expresses SaCas9 by the specific cTNT promoter and sgRNA targeted the mouse Camk2d gene by U6 promoter

Depositor

Yuxuan Guo

Article

Yang et al Circulation. 2024 May 28;149(22):1778-17

Insert

sgRNA

Use

AAV

Tags

HA and OLLAS

Promoter

cTNT

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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Human FLAG-WDR20

Plasmid

#217618

Purpose

Mammalian expression of FLAG tagged WDR20

Depositor

Ethan Lee

Article

Ng et al Nat Commun. 2023 Oct 5;14(1):6173. doi:

Insert

WDR20 (WDR20 Human)

Expression

Mammalian

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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iLID-tdTomato-Omp25

Plasmid

#214400

Purpose

Expresses fusion of iLID with tdTomato and transmembrane domain of Omp25

Depositor

Liting Duan

Article

Liu et al Proc Natl Acad Sci U S A. 2024 Apr 2;121

Insert

iLID-tdTomato-Omp25 (Synj2bp Synthetic, Rat)

Tags

iLID-TdTomato-Omp25

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-PARP1-delM43;F44I

Plasmid

#211579

Purpose

CMV driven PARP1-delM43;F44I -eGFP expressing construct with IRES-Neomycin selection

Depositor

Chris Lord

Article

Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

Insert

PARP1 (PARP1 Human)

Tags

eGFP

Expression

Mammalian

Mutation

Contains deletion of M43 and F44I

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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CHFR-PBZ-eGFP

Plasmid

#211581

Purpose

Expressing CHFR PBZ domain fused to eGFP

Depositor

Chris Lord

Article

Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

Insert

CHFR PBZ domain (CHFR Human)

Tags

eGFP

Expression

Mammalian

Mutation

PBZ domain of the protein

Promoter

CMV

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_EBF1_PDGFRB

Plasmid

#205807

Purpose

Express mEGFP-tagged fusion protein, EBF1_PDGFRB from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

EBF1_PDGFRB (EBF1, PDGFRB Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Jan. 5, 2024

Availability

Academic Institutions and Nonprofits only

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