We narrowed to 18,887 results for: nans

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pET-SOMA

Plasmid

#118935

Purpose

Used to express the SOMA FRET sensor in E. coli.

Depositor

Patrick Krysan

Article

Zaman et al Plant J. 2018 Nov 16. doi: 10.1111/tpj.1

Insert

SOMA FRET sensor for measuring MAPK activity in Arabidopsis thaliana

Expression

Bacterial

Available Since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pHCas9-Nours

Plasmid

#107733

Purpose

Escherichia coli- S. cerevisiae shuttle plasmid harbors a Cas9 gene, a natMX6 and URA3 selection markers used in S. cerevisiae

Depositor

Sheng Yang

Article

Wang et al Biotechnol Bioeng. 2018 Sep 10. doi: 10.

Insert

Cas9

Use

CRISPR

Tags

SV40 NLS

Expression

Yeast

Mutation

Human optimized

Promoter

TEF1

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pCS5

Plasmid

#55750

Purpose

Produces one half (beta) of aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.

Depositor

Matthew Bennett

Article

Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50

Insert

KanR

Expression

Bacterial

Mutation

Residues 59-264 of KanR

Promoter

T7-lac

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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pJBL705

Plasmid

#140375

Purpose

Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 5BP dowsntream from the promoter

Depositor

Julius Lucks

Article

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-5BP-tetO-3WJdB-T

Use

Synthetic Biology

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pMSCV FHA-ATG12

Plasmid

#27049

Depositor

Jayanta Debnath

Article

Radoshevich et al Cell. 2010 Aug 20. 142(4):590-600.

Insert

autophagy-related 12 (yeast) (Atg12 Mouse)

Use

Retroviral

Tags

HA and flag

Expression

Mammalian

Available Since

Jan. 13, 2011

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf137

Plasmid

#12825

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf137

Expression

Mammalian

Available Since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3.2 mir-1-1 reporter hsa-let-7a-1

Plasmid

#46670

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

hsa-let-7a-1 (MIRLET7A1 Human)

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2013

Availability

Academic Institutions and Nonprofits only

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pEN_TT Tgfbr3-HA

Plasmid

#58497

Purpose

Gateway entry vector for an inducible HA-tagged mouse Tgfbr3

Depositor

Kevin Janes

Article

Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

Insert

Tgfbr3_HA (Tgfbr3 Mouse)

Use

Entry vector

Tags

HA

Available Since

Aug. 12, 2014

Availability

Academic Institutions and Nonprofits only
DsRed2_pcDNA6.2/EmGFP-Bsd

Plasmid

#176988

Purpose

Mammalian expression vector encoding DsRed2 and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

DsRed2

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pDECKO_Malat1_E

Plasmid

#72624

Purpose

Expresses two gRNAs targeting MALAT1 promoter

Depositor

Roderic Guigo, Rory Johnson

Article

Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

Insert

gRNAs toward Malat1

Expression

Mammalian

Promoter

U6 (gRNA1) and H1 (gRNA2)

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti CRISPR V2 sgMTFMT_1

Plasmid

#106318

Purpose

Express Cas9 and sgRNA targeting MTFMT

Depositor

Richard Possemato

Article

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Insert

sgRNA targeting MTFMT

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPglu-600uDelta11 Aphrodite

Plasmid

#13569

Depositor

Wolf Frommer

Article

Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

Insert

FLIPglu F16A Delta11 Aphrodite

Tags

Aphrodite and ECFP

Expression

Bacterial

Mutation

Fluorophore-derived linker shortened. F16A mutati…

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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pMIII-Neo-E2Crimson

Plasmid

#153425

Purpose

Retroviral (MSCV) expression of E2-Crimson fluorescent protein

Depositor

Maki Nakayama

Article

Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

Insert

E2-Crimson

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pResQ shDna2'

Plasmid

#31952

Depositor

Sheila Stewart

Article

Duxin et al Mol Cell Biol. 2009 Aug;29(15):4274-82.

Insert

shRNA against hDna2 (DNA2 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
pLentiCRISPR sgSLC19A1

Plasmid

#102314

Purpose

genetic depletion of SLC19A1

Depositor

David Sabatini

Article

Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

sgSLC19A1 (SLC19A1 Human)

Use

Lentiviral

Promoter

U6

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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pBAD-SPW

Plasmid

#190628

Purpose

Bacterial expression of genetically encoded protease indicator SPW

Depositor

Robert Campbell

Article

Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

Insert

SPW

Expression

Bacterial

Available Since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp-DARPinOFF7

Plasmid

#182397

Purpose

Bacterial expression of Vesicle Nucleating peptide-DARPinOFF7 fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-DARPinOFF7

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pEF1α-owl-monkey-retroCHMP3(148)-HA

Plasmid

#154252

Purpose

expresses prematurely truncated owl monkey retroCHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(

Insert

retroCHMP3

Tags

HA

Expression

Mammalian

Mutation

C-terminal truncation of retroCHMP3 at amino acid…

Promoter

EF1-alpha

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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pCNL-N1_zyxin

Plasmid

#65682

Purpose

Expresses Zyxin-CNL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

zyxin (Zyx Mouse)

Use

Luciferase

Tags

CNL

Expression

Mammalian

Promoter

CMV

Available Since

June 17, 2015

Availability

Academic Institutions and Nonprofits only
pMT469

Plasmid

#139395

Purpose

Nanoluc placed on Output 1 of the 2in3out circuit (A001 and P002 design) with aTC and Bile Acid as inputs, AmpR, ErmR

Depositor

Christopher Voigt

Article

Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

Insert

Nanoluc placed on Output 1 of the 2in3out circuit (A001 and P002 design) with aTC and Bile Acid as inputs

Use

Synthetic Biology

Promoter

aTC and BA inducible promoters

Available Since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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