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mEosEM-Lamin A

Plasmid

#132707

Purpose

application in Correlative light-electron microscopy

Depositor

Pingyong Xu

Article

Fu et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

Insert

lamin A

Tags

mEosEM

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pBAD-pHuji

Plasmid

#61555

Purpose

Red fluorescent protein based pH indicator in bacterial expression vector

Depositor

Robert Campbell

Article

Shen et al J Cell Biol. 2014 Nov 10;207(3):419-32.

Insert

pHuji

Tags

6xHis

Expression

Bacterial

Available Since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only
pENTR TERRA

Plasmid

#111383

Purpose

TERRA with approximately 130 telomere hexanucleotide repeats of TTAGGG and TTGGGG variant sequences

Depositor

Klaus Holzmann

Article

Kreilmeier et al Genes (Basel). 2016 Aug 16;7(8). pii: ge

Insert

TERRA

Use

Gateway entry cloning

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pC1-HyPer-C199S

Plasmid

#42213

Purpose

Peroxide-insensitive version of HyPer to be used as a pH-control. Can be applied as a pH sensor.

Depositor

Vsevolod Belousov

Article

Belousov et al Nat Methods. 2006 Apr;3(4):281-6.

Insert

HYPER C199S

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pNM3732

Plasmid

#153086

Purpose

mec-4p GFP-C1 positive control for RMCE integration

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Insert

mec-4p GFP-C1 tbb-2 3'

Use

Rmce

Expression

Worm

Promoter

not applicable

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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CAPZB2-TwinStrepII

Plasmid

#83194

Purpose

Expression of tagged protein in mammalian cells

Depositor

Peter Barr-Gillespie

Insert

CAPZB2 (Capzb Mouse)

Tags

Twin Strep Tag II

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pIVEX-anchor

Plasmid

#46844

Purpose

Template for the amplification of spiking anchors in BeSD

Depositor

Florian Hollfelder

Article

Diamante et al Protein Eng Des Sel. 2013 Oct;26(10):713

Type

Empty backbone

Use

In vitro expression

Available Since

Aug. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pNM3810

Plasmid

#154119

Purpose

mec-4 promoter driving QF in an RCME vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Insert

mec-4p::QF::act-4 '3

Use

Rmce

Expression

Worm

Promoter

not applicable

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW mkok-T2A-mTurquoise2

Plasmid

#118428

Purpose

Produces equimolar expression of mKokappa and mTq2

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

mkok-T2A-mTq2

Expression

Yeast

Available Since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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POC1423

Plasmid

#221559

Purpose

Plasmid designed to assay the site-selective methylation protection approach using the non-switchable methylases

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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pHD-SPARC2-S-13xLexAop-GCaMP8m_VK05

Plasmid

#238557

Purpose

A LexAop-based SPARC plasmid for SPARC-L. Can also be used as a standalone sparsener of a LexA driver line. Sparse (S) density. The GCaMP ORF can be swapped for your gene of interest.

Depositor

Tom Clandinin

Article

TA et al Cell, CELL-D-24-04567, CELL13979

Insert

GCaMP8m

Use

Sparc plasmid

Tags

DsRed-Express

Expression

Insect

Available Since

May 23, 2025

Availability

Academic Institutions and Nonprofits only

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pHD-SPARC2-I-13xLexAop-GCaMP8m_VK05

Plasmid

#238556

Purpose

A LexAop-based SPARC plasmid for SPARC-L. Can also be used as a standalone sparsener of a LexA driver line. Intermediate (I) density. The GCaMP ORF can be swapped for your gene of interest.

Depositor

Tom Clandinin

Article

TA et al Cell, CELL-D-24-04567, CELL13979

Insert

GCaMP8m

Use

Sparc plasmid

Tags

DsRed-Express

Expression

Insect

Available Since

May 23, 2025

Availability

Academic Institutions and Nonprofits only

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psLIB TRE GTS-mTagBFP

Plasmid

#229969

Purpose

encodes a mTagBFP targeted to Golgi apparatus under the control of a dox inducible promoter

Depositor

Martin Pelosse

Article

Pelosse et al Synthetic Biology 2025.02.13

Insert

GTS-mTagBFP

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pQE-9-RTXWTM-X2S-C

Plasmid

#225967

Purpose

Block V Repeats-In-Toxin (RTX) protein with global substitution of serine in nonapeptide repeats and C-terminal Capping Domain

Depositor

Danielle Mai

Article

Chang et al Polym Chem. 2024. doi: https://doi.org/1

Insert

RTXWTM-X2S-C

Tags

6xHis

Expression

Bacterial

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pQE-9-RTXWTM-X2N-C

Plasmid

#225968

Purpose

Block V Repeats-In-Toxin (RTX) protein with global substitution of asparagine in nonapeptide repeats and C-terminal Capping Domain

Depositor

Danielle Mai

Article

Chang et al Polym Chem. 2024. doi: https://doi.org/1

Insert

RTXWTM-X2N-C

Tags

6xHis

Expression

Bacterial

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pQE-9-RTXWTM-X2D-C

Plasmid

#225969

Purpose

Block V Repeats-In-Toxin (RTX) protein with global substitution of aspartic acid in nonapeptide repeats and C-terminal Capping Domain

Depositor

Danielle Mai

Article

Chang et al Polym Chem. 2024. doi: https://doi.org/1

Insert

RTXWTM-X2D-C

Tags

6xHis

Expression

Bacterial

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pQE-9-RTXWTM-X2E-C

Plasmid

#225970

Purpose

Block V Repeats-In-Toxin (RTX) protein with global substitution of glutamic acid in nonapeptide repeats and C-terminal Capping Domain

Depositor

Danielle Mai

Article

Chang et al Polym Chem. 2024. doi: https://doi.org/1

Insert

RTXWTM-X2E-C

Tags

6xHis

Expression

Bacterial

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pQE-9-RTXMCR-TLY-X2A-C

Plasmid

#225971

Purpose

Repeats-In-Toxin (RTX) protein consensus sequence GGAGADTLY repeated nine times with C-terminal Capping Domain

Depositor

Danielle Mai

Article

Chang et al Polym Chem. 2024. doi: https://doi.org/1

Insert

RTMCR-TLY-X2A-C

Tags

6xHis

Expression

Bacterial

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pQE-9-RTXMCR-TLY-X2H-C

Plasmid

#225972

Purpose

Repeats-In-Toxin (RTX) protein consensus sequence GGAGHDTLY repeated nine times with C-terminal Capping Domain

Depositor

Danielle Mai

Article

Chang et al Polym Chem. 2024. doi: https://doi.org/1

Insert

RTXMCR-TLY-X2H-C

Tags

6xHis

Expression

Bacterial

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pQE-9-RTXMCR-TLY-X2S-C

Plasmid

#225973

Purpose

Repeats-In-Toxin (RTX) protein consensus sequence GGAGSDTLY repeated nine times with C-terminal Capping Domain

Depositor

Danielle Mai

Article

Chang et al Polym Chem. 2024. doi: https://doi.org/1

Insert

RTXMCR-TLY-X2S-C

Tags

6xHis

Expression

Bacterial

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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