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pMXS-IRES-Blast POLE1 N-ISCmut

Plasmid

#160803

Purpose

Express N-terminal ISC mutant POLE1

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

POLE1 N-ISCmut (POLE Human)

Use

Retroviral

Tags

3xFLAG

Mutation

shPOLE_1 and shPOLE2 resistant, C651S, C654S, C66…

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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c-Fos scFv [N486/76]

Plasmid

#190560

Purpose

Mammalian Expression of c-Fos scFV. Derived from hybridoma N486/76.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

c-Fos (Homo sapiens) recombinant scFV (FOS Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-EGFR-L747_T751delLREAT

Plasmid

#116270

Purpose

Lentiviral expression of EGFR L747_T751delLREAT

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

EGFR (EGFR Human)

Use

Lentiviral

Mutation

L747_T751delLREAT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-CDKN2A-A68T

Plasmid

#116220

Purpose

Lentiviral expression of CDKN2A A68T

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

CDKN2A (CDKN2A Human)

Use

Lentiviral

Mutation

A68T

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-Calretinin [L122/6R]

Plasmid

#177468

Purpose

Mammalian Expression Plasmid of anti-Calretinin (Human). Derived from hybridoma L122/6.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Calretinin (Homo sapiens) recombinant mouse monoclonal antibody (CALB2 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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NGFR 210Y177

Plasmid

#27487

Depositor

Warren Pear

Article

He et al Blood. 2002 Apr 15;99(8):2957-68.

Insert

bcr/abl (P210) (ABL1, BCR Human)

Use

Retroviral

Tags

IRES and NGFR

Expression

Mammalian

Mutation

Tyrosine 177 mutated to Phenylalanine (Y177F)

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only
pLEX307-mFzd9

Plasmid

#102877

Purpose

Expresses mouse Fzd9 in mammalian cells

Depositor

Henry Ho

Article

Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

Insert

Frizzled 9 (Fzd9 Mouse)

Use

Lentiviral

Expression

Mammalian

Mutation

A to G substitution at nucleotide 114, G to A sub…

Promoter

EF1A

Available Since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

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Anti-Nav1.8 Na+ channel [N134/12R]

Plasmid

#188203

Purpose

Mammalian Expression Plasmid of anti-Nav1.8 Na+ channel (Rat). Derived from hybridoma N134/12

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Nav1.8 Na+ channel (Rattus norvegicus) recombinant mouse monoclonal antibody (Scn10a Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-EGFR-R222C

Plasmid

#116293

Purpose

Lentiviral expression of EGFR R222C

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

EGFR (EGFR Human)

Use

Lentiviral

Mutation

R222C

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-EZH2-N322S

Plasmid

#116375

Purpose

Lentiviral expression of EZH2 N322S

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

EZH2 (EZH2 Human)

Use

Lentiviral

Mutation

N322S

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-BRAF-L597Q

Plasmid

#116177

Purpose

Lentiviral expression of BRAF L597Q

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Mutation

L597Q

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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B52 + FANCM sgSTOP

Plasmid

#100710

Purpose

B52 plasmid expressing FANCM sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)

Depositor

Alberto Ciccia

Article

Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

Insert

sgSTOP targeting FANCM (cloned using BbsI) (FANCM Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoters

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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Tet-O-FUW-NR3C1-P2A-mCherry

Plasmid

#203854

Purpose

Tet-inducible lentiviral plasmid expressing NR3C1-P2A-mCherry, with ORF specific barcode close to 3'LTR site

Depositor

Andrea Califano

Article

Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

Insert

NR3C1 (NR3C1 Human)

Expression

Mammalian

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-DDX3X-S412F

Plasmid

#116227

Purpose

Lentiviral expression of DDX3X S412F

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

DDX3X (DDX3X Human)

Use

Lentiviral

Mutation

S412F

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-DDX3X-E285K

Plasmid

#116226

Purpose

Lentiviral expression of DDX3X E285K

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

DDX3X (DDX3X Human)

Use

Lentiviral

Mutation

E285K

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CA-P539R

Plasmid

#116525

Purpose

Lentiviral expression of PIK3CA P539R

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CA (PIK3CA Human)

Use

Lentiviral

Mutation

P539R

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-EGFR-L747_P753delinsS

Plasmid

#116268

Purpose

Lentiviral expression of EGFR L747_P753delinsS

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

EGFR (EGFR Human)

Use

Lentiviral

Mutation

L747_P753delinsS

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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BKbeta2 K+ channel scFv [N53/32]

Plasmid

#190561

Purpose

Mammalian Expression of BKbeta2 K+ channel scFV. Derived from hybridoma N53/32.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

BKbeta2 K+ channel (Mus musculus) recombinant scFV (Kcnmb2 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-NSD1 [N312/10]

Plasmid

#190311

Purpose

Mammalian Expression Plasmid of anti-NSD1 (Human). Derived from hybridoma N312/10.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-NSD1 (Homo sapiens) recombinant Mouse monoclonal antibody (NSD1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-WRN [N428/12R]

Plasmid

#206575

Purpose

Mammalian Expression Plasmid of anti-WRN (Human). Derived from hybridoma N428/12.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-WRN (Homo sapiens) recombinant Mouse monoclonal antibody (WRN Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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