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pNHA_SBV_NSs

Plasmid

#208932

Purpose

Expresses N-terminally HA-tagged Schmallenberg orthobunyavirus NSs protein from CMV promoter in mammalian cells

Depositor

Gerald Barry

Insert

NSs

Tags

HA

Expression

Mammalian

Mutation

Deleted 50 bp from 5' end (including start c…

Promoter

CMV

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCDH-V2-CD20-Puro

Plasmid

#209756

Purpose

Lentiviral transfer plasmid to express the 5'-UTR and CDS of the human CD20 gene, MS4A1. Specifically, the variant 2 mRNA isoform.

Depositor

Andrei Thomas-Tikhonenko

Article

Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

Insert

MS4A1 (MS4A1 Human)

Use

Lentiviral

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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p164-pAAV-mDlx-FLEX-mScarlet

Plasmid

#204689

Purpose

Fluorescent reporter under the control of the Dlx promotor

Depositor

Lynette Lim

Article

Fisher et al Neuron. 2023 Dec 7:S0896-6273(23)00887-5

Insert

mScarlet

Use

AAV and Cre/Lox

Tags

mScarlet palmitoylation

Expression

Mammalian

Promoter

mDlx

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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tetO-MESP1

Plasmid

#170694

Purpose

doxycycline-inducible overexpression of MESP1

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

MESP1 (MESP1 Human)

Use

Lentiviral; Doxycycline inducible

Expression

Mammalian

Promoter

TRE promoter, Tet-ON

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pLEX303 TagRFP-T LGALS3 R186S

Plasmid

#202428

Purpose

Expression of TagRFP-T-tagged Galectin 3 with mutation of carbohdrate recognition binding domain (CRD) (R186S mutant)

Depositor

David Bryant

Article

Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

Insert

LGALS3 (LGALS3 Human)

Use

Lentiviral

Tags

TagRFP

Mutation

R186S

Available Since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pQCXIZ GFP PODXL FKBP 4K>R

Plasmid

#202421

Purpose

Expression of ubiquitination-deficient GFP-tagged PODXL with FKBP-rapamycin binding (FRB) dimerization domain

Depositor

David Bryant

Article

Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

Insert

PODXL (PODXL Human)

Use

Lentiviral

Tags

FKBP and GFP

Mutation

K431/525/526/547R

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pSLIK 3xFLAG-KPNA1 zeo

Plasmid

#192329

Purpose

Lentiviral expression vector for an inducible 3xFLAG-KPNA1

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

KPNA1 (KPNA1 Human)

Use

Lentiviral

Tags

3xFLAG

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pSLIK 3xFLAG-BCAS2 zeo

Plasmid

#192319

Purpose

Lentiviral expression vector for an inducible 3xFLAG-BCAS2

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

BCAS2 (BCAS2 Human)

Use

Lentiviral

Tags

3xFLAG

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pSLIK 3xFLAG-KIF20A zeo

Plasmid

#192322

Purpose

Lentiviral expression vector for an inducible 3xFLAG-KIF20A

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

KIF20A (KIF20A Human)

Use

Lentiviral

Tags

3xFLAG

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3xFLAG-KPNA1

Plasmid

#192304

Purpose

Gateway entry vector for an inducible 3xFLAG-KPNA1

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

KPNA1 (KPNA1 Human)

Use

Gateway Cloning

Tags

3xFLAG

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MUC2 D1 with cleavable His tag

Plasmid

#181813

Purpose

Expresses human MUC2 D1 with cleavable His tag, residues 21-389

Depositor

Deborah Fass

Article

Reznik et al Cell. 2022 Oct 27;185(22):4206-4215.e11.

Insert

Muc2 D1 (MUC2 Human)

Tags

6xHistadine followed by TEV cleavage site

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-CBS (H65R)

Plasmid

#182924

Purpose

Mammalian expression plasmid for human CBS mutant H65R

Depositor

Philip Eaton

Article

Switzer et al Proc Natl Acad Sci U S A. 2023 Jan 17;12

Insert

Cystathionine Beta-Synthase (CBS Human)

Tags

eGFP

Expression

Mammalian

Mutation

changed histidine 65 to arginine

Promoter

CMV

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003 sgNFkB2 guide 1

Plasmid

#193593

Purpose

NFkB2 knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgNFkB2 guide 1 (NFKB2 Human)

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003 sgNFkB2 guide 2

Plasmid

#193594

Purpose

NFkB2 knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgNFkB2 guide 2 (NFKB2 Human)

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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SFRS10

Plasmid

#156007

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

SFRS10 (TRA2B Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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CCNL2

Plasmid

#155441

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

CCNL2 (CCNL2 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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PRPF40A

Plasmid

#155851

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

PRPF40A (PRPF40A Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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SFRS17A-1

Plasmid

#156011

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

SFRS17A (SFRS17A Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pET21a Lm-iPGM

Plasmid

#180242

Purpose

Bacterial expression plasmid for production of recombinant Leishmania mexicana iPGM His10

Depositor

James Inglese

Article

van Neer et al ACS Chem Biol. 2022 Aug 19;17(8):2284-22

Insert

Leishmania mexicana iPGM-10His

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pET21a Wb-iPGM

Plasmid

#180246

Purpose

Bacterial expression plasmid for production of recombinant Wuchereria bancrofti iPGM His10

Depositor

James Inglese

Article

van Neer et al ACS Chem Biol. 2022 Aug 19;17(8):2284-22

Insert

Wuchereria bancrofti iPGM-10His

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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