We narrowed to 24,518 results for: FUS
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Plasmid#135554PurposeExpress rat Munc18b in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Munc18b.DepositorInsertMunc18b (Stxbp2 Rat)
Tags6xHis tag and TEV siteExpressionInsectPromoterpolyhedrin promoterAvailable SinceMarch 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET24a-LaM4-1xTS
Plasmid#162778PurposeBacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to one tyrosine sulfation (TS) motif. LaM4-1xTS also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-mCherry nanobody fused to a TS site, T7, HA, BAP and His6 epitope
TagsBAP, HA, His6, T7, and TS site from proCCKExpressionBacterialPromoterT7Available SinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPLN-hFluc-DM-eGFP-KDEL
Plasmid#177728PurposeDM Firefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention and fused to eGFP at the C-terminusDepositorInserthFlucDM
TagsKDEL, Prolactin Signal Sequence, and eGFPExpressionBacterial and MammalianMutationR188Q, R261Q (Destabilized Mutant)Available SinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-M.Mus.1
Plasmid#196681PurposeRep/Cap plasmid for the production of M.Mus.1, an AAV capsid with CNS tropism in mice.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVMutationWVLPSGG insert between amino acids 588 and 589 of…Promoterp41Available SinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-MDV1B
Plasmid#196680PurposeRep/Cap plasmid for the production of MDV1B, an AAV capsid with CNS tropism in mice.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVMutationKTVGTVY insert between amino acids 588 and 589 of…Promoterp41Available SinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
DsRed +9e
Plasmid#191766PurposeExpression of DsRed fused to a positively charged polypeptide (2 x KSG) . Overall charge on tetramer: +9eDepositorInsertDsRed
TagsN terminal fusion of positively charged polypetod…ExpressionBacterial and MammalianPromoterCMVAvailable SinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
siRNA-resistant ABHD16A-mCherry
Plasmid#195161Purposemammalian expression of siRNA-resistant ABHD16A tagged with mCherryDepositorInsertABHD16A (ABHD16A Human)
TagsmCherryExpressionMammalianMutationWobble mutations for siRNA resistance from L90 to…PromoterCMVAvailable SinceFeb. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
siRNA-resistant ABHD16A-SNAP
Plasmid#195164Purposemammalian expression of siRNA-resistant ABHD16A tagged with SNAP tagDepositorInsertABHD16A (ABHD16A Human)
TagsSNAP tagExpressionMammalianMutationWobble mutations for siRNA resistance from L90 to…PromoterCMVAvailable SinceFeb. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNAS1b split mCherry NEDD4-2 WW2
Plasmid#111883PurposeMode #2 phosphosite expression (split mCherry, using NEDD4-2 WW2 binding domain)DepositorTypeEmpty backboneTagsSplit mCherry system: NEDD4-2 WW2 fused to C-term…ExpressionBacterialAvailable SinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA GAL4 CREB 1-341 M1
Plasmid#22973DepositorInsertCREB (Creb1 GAL4-fusion, Rat)
TagsGAL4 DNA-binding domainExpressionMammalianMutationChanged Serine 133 to Alanine of CREBAvailable SinceFeb. 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
pONSY-Lifeact:mCherry
Plasmid#111876PurposeThis plasmid expresses mCherry fused to Lifeact peptide. It labels actin bundles and filopodia in Capsaspora. Codons have been optimised according to their usages in Capsaspora and C. fragrantissima.DepositorInsertLifeact fused to mCherry
UseCapsaspora owczarzakiTagsmCherryPromoterElongation Factor 1 alpha (EF1a) from CapsasporaAvailable SinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
8200 Bicistronic_GFP_ires_hygro
Plasmid#64375PurposeThis a retroviral expression plasmid expressing GFP along with hygro resistance geneDepositorInsertEGFP
UseRetroviralTagsEGFPAvailable SinceJuly 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
(81) pcDNA3.1-BC2-Nup62-Flag-EPEA
Plasmid#168094PurposeNucleoporin Nup62 with N-terminal BC2 nanobody tag and C-terminal Flag and EPEA nanobody tagsDepositorInsertNup62-Ubc-Flag-EPEA
TagsBC2-Tag, EPEA-tag, and Flag-tagExpressionMammalianMutationP229A, S283T on Nup62 native sequencePromoterT7/CMVAvailable SinceMay 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
TtGNN-TAF4-HFD
Plasmid#106381Purposedox inducible expression of GFP fused TAF4 HFD (827-909) with neo resistanceDepositorInsertGFP fused TAF4 HFD (827-909) (Taf4 Mouse)
UseRetroviralTagsGFPExpressionMammalianPromoterTREtightAvailable SinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCD5-ss-D/bovine/France/5920/2014-HEFs57aED-GCN4-sfGFP-ST
Plasmid#175018PurposeExpresses influenza D virus trimeric hemagglutinin esterase fusion protein fused to a sfGFPDepositorInsertOK-HEF s57a ED
TagsGCN4-TEV-sfGFP-TwinStrepExpressionMammalianMutations57a esterase knock out mutantPromoterCMVAvailable SinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRAN357
Plasmid#120898PurposeE. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of cyan fluorescent protein (CfpOpt), multiple-cloning site for fusion to N-terminus of target proteinDepositorInsertcyan fluorescent protein, codon-optimized for low GC organisms, multiple-cloning site for protein fusion
ExpressionBacterialPromoterPtetAvailable SinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-X1-Neo-HA-UFL1-RAMP4
Plasmid#139863PurposeLentiviral expression of fusion of human UFL1 (full length) with RAMP4DepositorInsertUFL1-RAMP4 (UFL1 Human)
UseLentiviralTagsHAMutationrecombinant fusion of UFL1 (full length) with RAM…Available SinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dLbCpf1-BE0ΔiNLS
Plasmid#107684PurposeExpresses pCMV-dLbCpf1-BE0ΔiNLS crRNA in mammalian cellsDepositorInsertdLbCpf1-BE0ΔiNLS
ExpressionMammalianMutationD832A/E925A/D1148APromoterCMVAvailable SinceApril 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
FN(9-10)-I27-CC
Plasmid#85394PurposeMTFM mechanosensor for integrin. Fibronectin domains 9&10 fused to titan immunoglobulin domain (I27) with two cysteines for immobilization, site for p-azidophenylalanine incorporation & Cy3 labelingDepositorInsertFibronectin (FN) domains 9 & 10 for integrin binding fused to titin immunoglobulin domain (I27), containing a TAG (amber) codon
Tags6x HisExpressionBacterialPromoterT7Available SinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-TP1
Plasmid#160084PurposeBacterial expression of Tn5-APEX2 fusion protein, no linkerDepositorInsertTn5 transposase/APEX2 peroxidase fusion protein
TagsFLAG and Mxe intein - Chitin-binding domainExpressionBacterialAvailable SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only