We narrowed to 12,205 results for: LEA
-
Plasmid#221198PurposeScChd1-aa118-1274 chromatin remodeler with Y137A mutationDepositorInsertScChd1 (aa118-1274)
Tags6xHis-tag (Precission protease cleavable)ExpressionBacterialMutationY137APromoterT7Available sinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-ArgiNLS-miRFP670
Plasmid#220600PurposeConstitutive expression of a single-cell discriminating version of miRFP670 fluorescent protein.DepositorInsertmiRFP670
UseAAVTagsArgiNLSExpressionMammalianPromoterEF1aAvailable sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAGK43
Plasmid#222223PurposeEdits NPAS2 Gene.DepositorAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPCaspase
Plasmid#214042PurposeBacterial expression plasmid for PCaspase from Haliangium ochraceumDepositorInsertPCaspase
ExpressionBacterialMutationWTPromoterlacUV5 promoterAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSAVED-CHAT_PCaspase-C-term
Plasmid#214045PurposeBacterial expression plasmid for SAVED-CHAT and PCaspase C-terminal fragment (aa 154-666) from Haliangium ochraceumDepositorInsertSAVED-CHAT-PCaspase
ExpressionBacterialMutationaa 154-666PromoterlacUV5 promoterAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
GRPR-DuET
Plasmid#213306PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCL1315
Plasmid#217957PurposeExpression of human H3.1 carrying a triple point mutation to disrupt folding with histone H4. Expression in mammalian cells driven by the CMV promoter.DepositorInsertHistone H3.1 (H3C1 Human)
TagseGFPExpressionMammalianMutationF84A, L92A, Y99APromoterCMVAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMAT_M4_YVTM_YQNQ
Plasmid#197084PurposeThis plasmid contains the coding sequence for signaling peptide M4, which contains the YVTM and YQNQ signaling motifs. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFPDepositorInsertThis plasmid contains the coding sequence for signaling peptide M4, which contains the YVTM and YQNQ signaling motifs.
ExpressionMammalianAvailable sinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMAT_M10_PQQAT
Plasmid#197093PurposeThis plasmid contains the coding sequence for signaling peptide M10, which contains the PQQAT signaling motif. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFPDepositorInsertThis plasmid contains the coding sequence for signaling peptide M10, which contains the PQQAT signaling motif.
ExpressionMammalianAvailable sinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMAT_M11_PQEINF
Plasmid#197094PurposeThis plasmid contains the coding sequence for signaling peptide M11, which contains the PQEINF signaling motif. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFPDepositorInsertThis plasmid contains the coding sequence for signaling peptide M11, which contains the PQEINF signaling motif.
ExpressionMammalianAvailable sinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMAT_M12_RPTAVEG
Plasmid#197095PurposeThis plasmid contains the coding sequence for signaling peptide M12, which contains the RPTAVEG signaling motif. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFPDepositorInsertThis plasmid contains the coding sequence for signaling peptide M12, which contains the RPTAVEG signaling motif.
ExpressionMammalianAvailable sinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMAT_M13_6xSAG
Plasmid#197096PurposeThis plasmid contains the coding sequence for the control peptide M13, which contains a linker with six repeats of serine-alanine-glycine. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CDepositorInsertControl peptide M13, which contains a linker with six repeats of serine-alanine-glycine (SAG)
ExpressionMammalianAvailable sinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28_Caspase-4_W267N
Plasmid#214314PurposeProtein overexpression in bacteriaDepositorAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28_Caspase-4_R269D
Plasmid#214313PurposeProtein overexpression in bacteriaDepositorAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT7CFE1_EVD68_3ABC_C147R
Plasmid#212978PurposeEncodes EV-D68 MO/14/18947 3ABC with active site mutation optimized for expression in mammalian transcription/translation extractsDepositorInsertEV-D68 MO/14/18947 3ABC C147R
UseMammalian cell free protein expressionTagsHAMutationchanged Cysteine 147 in 3C to ArgininePromoterT7Available sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT7CFE1_EVD68_VP12A_N84T
Plasmid#212983PurposeEncodes EV-D68 MO/14/18947 VP12A with telaprevir resistance mutation optimized for expression in mammalian transcription/translation extractsDepositorInsertEV-D68 MO/14/18947 VP12A N84T
UseMammalian cell free protein expressionTagsHAMutationchanged Asparagine 84 from 2A to ThreoninePromoterT7Available sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-15b-aδNb072
Plasmid#204627PurposeExpresses the anti-IgD nanobody 072 (aδNb072) in the bacterial periplasm. aδNb072 is an orthosteric inhibitor of human anti-Phl p 7 antibodies of clone 102.1F10.DepositorInsertaδNb072
TagsHis6 and TEV protease cleavage siteExpressionBacterialPromoterT7Available sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEF mito-PAcGFP
Plasmid#188905PurposeExpresses mitochondrial photoactivatable GFPDepositorInsertmitochondrial photoactivatable GFP
TagsMitochondrial leader sequence: MSVLTPLLLRGLTGSARR…ExpressionMammalianAvailable sinceOct. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
MLKP1delTM
Plasmid#130841PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertMLKP1
TagseGFP-FLAG-HAExpressionPlantMutationDeletion of transmembrane domainPromoterCaMV 35SAvailable sinceSept. 28, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
MLKP1del4ec
Plasmid#130844PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertMLKP1
UseGateway CloningTagseGFP-FLAG-HAMutationDeletion of terminal four amino acid residuesAvailable sinceSept. 28, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits