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pFPcR5

Plasmid

#149484

Purpose

Broad-Host range expression vector, RSF1010 origin, PcaU(AM) repressor, P3B5 promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

PcaU(AM)-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

P3B5B

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pQLinkHD-SYNZIP3

Plasmid

#80659

Purpose

Encodes the synthetic coiled coil peptide SYNZIP3

Depositor

Amy Keating

Article

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP3

Tags

6xHis

Expression

Bacterial

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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LP539

Plasmid

#90203

Purpose

pCMV-myc with cDNA encoding HsTRAPPC8 aa residues 1-700

Depositor

Lotte Pedersen

Article

Verdier et al Methods Mol Biol. 2016;1454:15-33. doi:

Insert

TRAPPC8 (TRAPPC8 Human)

Tags

Myc

Expression

Mammalian

Mutation

aa residues 1-700

Promoter

CMV

Available Since

June 2, 2017

Availability

Academic Institutions and Nonprofits only

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601-145-8X

Plasmid

#158620

Purpose

DNA fragment production for nucleosome core particle preparation

Depositor

Curt Davey

Article

Vasudevan et al J Mol Biol. 2010 Oct 15;403(1):1-10. doi

Type

Empty backbone

Use

Bacterial vector for dna production

Available Since

Nov. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pJA255

Plasmid

#21508

Depositor

Julie Ahringer

Article

Zeiser et al PLoS One. 2011;6(5):e20082. Epub 2011 Ma

Insert

pmex-5::citrine (w introns)::(Gly)5Ala

Use

Multisite gateway

Available Since

Aug. 28, 2009

Availability

Academic Institutions and Nonprofits only

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PSD95-TS:YSOG2

Plasmid

#42227

Depositor

Roger Tsien

Article

Butko et al Nat Neurosci. 2012 Nov;15(12):1742-51. d

Insert

PSD95

Tags

TS:YSOG2

Expression

Mammalian

Available Since

March 15, 2013

Availability

Academic Institutions and Nonprofits only

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pQE-WT-CGG-R12-KIR

Plasmid

#63626

Purpose

CGG-R12-KIR RNAP mutant

Depositor

Andrew Ellington

Article

Meyer et al ACS Synth Biol. 2014 Oct 7.

Insert

K1F IKR RNAP

Mutation

Q744K, L747V, N748H, L749I, R756E, L757M, H772R

Promoter

T5/Lac

Available Since

July 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pMAL658

Plasmid

#167837

Purpose

Encodes OptoLAC1B circuit

Depositor

Jose Avalos

Article

Lalwani et al Nat Chem Biol. 2021 Jan;17(1):71-79. doi

Insert

lacI

Use

Synthetic Biology

Tags

ssrA (LAA)

Expression

Bacterial

Available Since

Sept. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pMAL659

Plasmid

#167838

Purpose

Encodes OptoLAC2B circuit

Depositor

Jose Avalos

Article

Lalwani et al Nat Chem Biol. 2021 Jan;17(1):71-79. doi

Insert

lacI

Use

Synthetic Biology

Tags

ssrA (AAV)

Expression

Bacterial

Available Since

Sept. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pFCiK5

Plasmid

#167514

Purpose

Broad-Host range expression vector, RSF1010 origin, CinRAM activator, PCin promoter, mKelly2, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

CinRAM-mKelly2

Use

Synthetic Biology

Expression

Bacterial

Promoter

PCin

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAB147

Plasmid

#101662

Purpose

Opto-T7RNAP*(302)

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(302)

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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negEKARet-cyto

Plasmid

#114734

Purpose

Negative control of EKARet-cyto

Depositor

Ryohei Yasuda

Article

Tang et al Neuron. 2017 Mar 22;93(6):1315-1324.e3.

Insert

Negative Control of EKARet-cyto

Tags

EGFP and sREAChet

Expression

Mammalian

Promoter

CMV

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pJA258

Plasmid

#21511

Depositor

Julie Ahringer

Article

Zeiser et al PLoS One. 2011;6(5):e20082. Epub 2011 Ma

Insert

(Gly)5Ala::citrine(w introns)::tbb-2 3'UTR

Use

Multisite gateway

Available Since

Aug. 28, 2009

Availability

Academic Institutions and Nonprofits only

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pNXRVj-HACre

Plasmid

#78407

Purpose

constitutive Cre expression vector

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

3xHA-nls-Cre
aur1R (aur1 Fission Yeast)

Expression

Yeast

Mutation

Gly 240 to Cys

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR-R4r-HCN1miR-EGFP-R3r

Plasmid

#32586

Depositor

Matthew Nolan

Article

White et al Front Mol Neurosci. 2011;4:8. Epub 2011

Insert

Intronic HCN1miRNA and EGFP

Use

Gateway cloning vector

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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pMT Draper-mCherry

Plasmid

#126667

Purpose

Inducible expression of Draper-mCherry in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Draper mCherry

Expression

Insect

Mutation

C813Y- see depositor comments below

Available Since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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pQE-WT-N4-R5-RNRV

Plasmid

#63630

Purpose

N4-R5-RNRV RNAP mutant

Depositor

Andrew Ellington

Article

Meyer et al ACS Synth Biol. 2014 Oct 7.

Insert

N4-R5-RNRV RNAP

Mutation

L747I, N748D, L749C, M750V, F751I, Q754T, F755R, …

Promoter

T5/Lac

Available Since

April 15, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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p3E LAP2b

Plasmid

#105978

Purpose

3' element for Gateway cloning, lamina-associated polypeptide 2

Depositor

Caren Norden

Article

Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

Insert

LAP2b

Expression

Bacterial

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pBVR5

Plasmid

#167511

Purpose

Broad-Host range expression vector, pBBR origin, VanRAM repressor, PVanCC promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

VanRAM-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PVanCC

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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spoT_NS1

Plasmid

#100914

Purpose

expresses spoT (from native promoter) from the NS1 site in the S. elongatus PCC7942 genome

Depositor

Erin O'Shea

Article

Puszynska et al Cell Rep. 2017 Dec 12;21(11):3155-3165.

Insert

synpcc7942_1377 (spoT)

Use

Cyanobacteria cloning vector

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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