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pHH0103_SORBS1-2/3

Plasmid

#91530

Purpose

Protein expression and purification of human SH3 domain construct SORBS1-2/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SORBS1-2/3 (SORBS1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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FKBP-YPet-RanGAP*

Plasmid

#175245

Purpose

Bacterial expression and purification, SUMOylation substrate that can be recruited to FRB with rapamycin, Ypet is a FRET acceptor for CyPet

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

RANGAP1 (RANGAP1 Human)

Expression

Bacterial

Mutation

Amino acid 562 F to A

Promoter

tac

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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ABE8e(TadA-8e K20A R21A)

Plasmid

#138496

Purpose

A-to-G base editor

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

ecTadA(8e K20A R21A)-nSpCas9

Expression

Mammalian

Mutation

TadA-8e K20A R21A

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti6.3/V5-DEST-PKCi-A120E-ΔPB1

Plasmid

#56658

Purpose

expresses constitutively active human PKCi with a deletion on the PB1 domain

Depositor

Pedro Salas

Article

Forteza et al Biol Open. 2013 Oct 8;2(11):1264-9. doi:

Insert

Human PKCi (PRKCI Human)

Use

Lentiviral

Tags

V5/His

Mutation

A120E and deletion of PB1 domain (aa 24–98)

Promoter

CMV

Available Since

Sept. 3, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO V5 DNAJB5

Plasmid

#19527

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DnaJ (Hsp40) homolog, subfamily B, member 5 (DNAJB5 Human)

Tags

V5

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-AGM-TAN1 vpr

Plasmid

#115819

Purpose

Encodes codon optimized 3xFLAG-SIVAGM Vpr (TAN1) (U58991) with repaired premature stop codon and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVAGM Vpr (TAN1)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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psiCHECK CHAC1 3'UTR

Plasmid

#19861

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Insert

CHAC1 3'UTR (CHAC1 Human)

Expression

Mammalian

Available Since

Jan. 12, 2009

Availability

Academic Institutions and Nonprofits only

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NG1237 pCI-TPI-PTC160-4H

Plasmid

#65804

Purpose

NMD reporter mRNA

Depositor

Niels Gehring

Article

Boehm et al Cell Rep. 2014 Oct 23;9(2):555-68. doi:

Insert

TPI (TPI1 Human)

Tags

4H (4 copies of short beta-globin 3'UTR for …

Expression

Mammalian

Mutation

premature stop codon at 160

Promoter

CMV

Available Since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

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px330 MAU2 gRNA

Plasmid

#165589

Purpose

Insertion of MAU2 degron

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

MAU2 (CTCF Mouse)

Expression

Mammalian

Mutation

NA

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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pLENTI_RPS3_K214R_Keima

Plasmid

#127143

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

RPS3_Keima (RPS3 Human)

Use

Lentiviral

Mutation

K214R

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pBABE human IRS-1 CAAX

Plasmid

#11237

Depositor

Ronald Kahn

Article

Kriauciunas et al Mol Cell Biol. 2000 Sep . 20(18):6849-59

Insert

IRS-1 (IRS1 Human)

Use

Retroviral

Tags

CAAX

Expression

Mammalian

Mutation

C-terminal prenylation motif makes IRS-1 localize…

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA1 3XFlag ATXN1[15Q]-delAXH

Plasmid

#33241

Depositor

Huda Zoghbi

Article

Lam et al Cell. 2006 Dec 29;127(7):1335-47.

Insert

ATXN1[2Q]-delAXH (ATXN1 Human)

Tags

3xflag

Expression

Mammalian

Promoter

CMV

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only
E1b-GFP-Tol2-Gateway dre SE-zic2a S

Plasmid

#89388

Purpose

Vector for reporter assay of one zebrafish super-enhancer region

Depositor

Alena Shkumatava

Article

Perez-Rico et al Genome Res. 2017 Feb;27(2):259-268. doi:

Insert

zic2a (zic2a Zebrafish)

Available Since

June 15, 2017

Availability

Academic Institutions and Nonprofits only
pAAVsc.U6-sgFah.CMV/CB-EGFP

Plasmid

#121508

Purpose

Expresses sgRNA targeting mouse Fah intron 7 (sgFah).

Depositor

Guangping Gao

Article

Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

Insert

sgFah

Use

AAV

Expression

Mammalian

Promoter

U6

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pLV Tmem53-V5

Plasmid

#175142

Purpose

Lentiviral expression of mouse Tmem53-V5

Depositor

Larry Gerace

Article

Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

Insert

Tmem53 (Tmem53 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pET19b-hMED9

Plasmid

#15365

Depositor

Joan Conaway, Ronald Conaway

Article

Paoletti et al Proc Natl Acad Sci U S A. 2006 Dec 12. 1

Insert

hMED9 (MED9 Human)

Tags

His

Expression

Bacterial

Mutation

N/A

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
AcrIIC1X

Plasmid

#128112

Purpose

AcrX expression in mammalian cells; AcrX is an engineered anti-CRISPR protein targeting S. aureus Cas9

Depositor

Dominik Niopek

Article

Mathony et al Nat Chem Biol. 2020 Apr 13. pii: 10.1038

Insert

AcrX (AcrIIC1 N3F/D15Q/A48I)

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pMflT-o4

Plasmid

#101312

Purpose

Contains: complete oriC region of M. florum L1 (oriC4: rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA), colE1 rep origin, recoded tetM resistance gene, origin of transfer of RP4 plasmid.

Depositor

Sébastien Rodrigue

Article

Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7

Insert

oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)

Use

Synthetic Biology

Expression

Bacterial

Available Since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pCIneo-V5-SBP-HsSmg6-del36-576-siRNAres_I

Plasmid

#146553

Purpose

Mammalian Expression of HsSmg6-del36-576-siRNAres

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsSmg6-del36-576-siRNAres (SMG6 Human)

Expression

Mammalian

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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R4pGWB601_UBQ10p-BirA(R118G)-NES-YFP

Plasmid

#127363

Purpose

Binary vector for expressing cytosolic BirA* (R118G)-YFP under the UBQ10 promoter in plants

Depositor

Dominique Bergmann

Article

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

BirA* (R118G mutant)

Tags

GS linker, NES, V5, and YFP

Expression

Plant

Mutation

R118G

Promoter

Arabidopsis UBQ10 promoter

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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