We narrowed to 16,144 results for: LEA

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4QPP

Plasmid

#61964

Purpose

Baculovirus expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

HRMT1L6:PBC001-A12:C213642 (PRMT6 Human)

Use

Baculovirus expression

Tags

6x His and TEV cleavage site

Mutation

See comments

Promoter

Polyhedrin

Available Since

Sept. 11, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pNIC-PelB-NH10T-AVITAG-GG

Plasmid

#215793

Purpose

Cloning of target gene with N terminal PelB SEC pathway signal peptide His10 and AVITAG and TEV cleavage

Depositor

ASAP Discovery, Frank von Delft

Article

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Expression

Bacterial

Available Since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pBXC3H

Plasmid

#47068

Purpose

FX cloning E.coli expression vector with araBAD promoter and C-terminal 3C protease cleavage site and 10x His tag

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Bacterial

Promoter

araBAD

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pGBW-m4134397

Plasmid

#152706

Purpose

Mammalian expression plasmid for SARS-CoV-2 nsp9

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp9 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;HA

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available Since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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p7XC3GH

Plasmid

#47066

Purpose

FX cloning E.coli expression vector with T7 promoter and C-terminal 3C protease cleavage site, GFP and 10x His tags

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site), GFP, and dec…

Expression

Bacterial

Promoter

T7

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pGBW-m4134251

Plasmid

#152087

Purpose

Mammalian expression plasmid for SARS-CoV-2 nsp7

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp7 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;3xFlag

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available Since

May 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pAS_MmaPylT_EF1_NES-MmaPylRS AF

Plasmid

#174902

Purpose

expression of Mma PylRS AF with nuclear export sequence for amber suppression in mammalian cells; transient or piggy bac mediated integration

Depositor

Simon Elsaesser

Insert

Mma PylRS (pylS Methanosarcina mazei)

Tags

FLAG and NES

Expression

Mammalian

Mutation

Y306A, Y384F

Promoter

EF1

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pGBW-m4133643

Plasmid

#152361

Purpose

Mammalian expression plasmid for SARS-CoV-2 nsp6

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp6 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;3xFlag

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available Since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4134063

Plasmid

#152631

Purpose

Mammalian expression plasmid for SARS-CoV-2 nsp6

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp6 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;HA

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available Since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4134375

Plasmid

#152788

Purpose

Mammalian expression plasmid for SARS-CoV-2 N (nucleocapsid)

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 N (nucleocapsid) (N Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;HA

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available Since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4134072

Plasmid

#152082

Purpose

Mammalian expression plasmid for SARS-CoV-2 nsp4

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp4 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;3xFlag

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available Since

May 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4134545

Plasmid

#152594

Purpose

Mammalian expression plasmid for SARS-CoV-2 N (nucleocapsid)

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 N (nucleocapsid) (N Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;V5

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available Since

July 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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WDR61

Plasmid

#28162

Purpose

Baculovirus expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

WD repeat domain 61 (SKIC8 Human)

Use

Baculovirus expression

Tags

His tag with TEV cleavage

Promoter

polyhedrin

Available Since

Aug. 8, 2012

Availability

Industry, Academic Institutions, and Nonprofits
pGBW-m4134256

Plasmid

#152522

Purpose

Mammalian expression plasmid for SARS-CoV-2 helicase

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 helicase (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;3xFlag

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available Since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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XLone-BSD NFIL3 P2A eGFP NLS

Plasmid

#140030

Purpose

Tunable and temporal expression control of NFIL3 and nuclear eGFP

Depositor

Xiaoping Bao

Insert

NFIL3 (NFIL3 Synthetic, Human)

Use

Synthetic Biology

Tags

eGFP

Expression

Mammalian

Promoter

TRE3G

Available Since

May 24, 2021

Availability

Academic Institutions and Nonprofits only

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pGBW-m4133554

Plasmid

#152860

Purpose

Mammalian expression plasmid for SARS-CoV-2 M (membrane)

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;HA

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available Since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4134114

Plasmid

#152632

Purpose

Mammalian expression plasmid for SARS-CoV-2 nsp9

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp9 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;3xFlag

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available Since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pGBW-m4133607

Plasmid

#152942

Purpose

Mammalian expression plasmid for SARS-CoV-2 M (membrane)

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;HA

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available Since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pGBW-m4133178

Plasmid

#152147

Purpose

Mammalian expression plasmid for SARS-CoV-2 nsp8

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp8 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;3xFlag

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available Since

May 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLIC.B2.MJ0936

Plasmid

#11538

Depositor

Sung-Hou Kim

Article

Chen et al J Biol Chem. 2004 Jul 23. 279(30):31854-

Insert

phosphodiesterase

Tags

HisTag, non-cleavable

Expression

Bacterial

Available Since

April 10, 2006

Availability

Academic Institutions and Nonprofits only