We narrowed to 9,493 results for: pho;

1. DNMT3ACD-CRY2-EGFP

   Plasmid

   #82556

   Purpose

   Encodes DMNT3 under LITE2.0 system

   Depositor

   Joseph Irudayaraj

   Article

   Lo et al Sci Rep. 2017 Feb 9;7:42047. doi: 10.103

   Inserts

   DNMT3A (DNMT3A Human)
   CRY2 (CRY2 Mustard Weed)
   EGFP
   

   Use

   AAV

   Tags

   2A, NLS(alpha-imp), and NLS-VP64

   Expression

   Mammalian

   Mutation

   CRY2PHR (N-terminal photolyase homology region do…

   Promoter

   EF-1alpha

   Available Since

   Sept. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. TET1CD-CRY2-EGFP

   Plasmid

   #82555

   Purpose

   Encodes TET1 under LITE2.0 system

   Depositor

   Joseph Irudayaraj

   Article

   Lo et al Sci Rep. 2017 Feb 9;7:42047. doi: 10.103

   Inserts

   TET1 (TET1 Human)
   CRY2 (CRY2 Mustard Weed)
   EGFP
   

   Use

   AAV

   Tags

   2A, NLS(alpha-imp), and NLS-VP64

   Expression

   Mammalian

   Mutation

   CRY2PHR (N-terminal photolyase homology region do…

   Promoter

   EF-1alpha

   Available Since

   Sept. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1/TO-myc-BirA*-cCE

   Plasmid

   #112713

   Purpose

   For tetracycline-inducible expression of myc-BirA*-NES-mCE ΔNLS ("myc-BirA*-cCE") in mammalian cells. For BioID studies.

   Depositor

   Daniel Schoenberg

   Article

   Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1

   Insert

   Rngtt (Rngtt Mouse)
   

   Tags

   BirA* (BirA R118G), HIV Rev nuclear localization …

   Expression

   Mammalian

   Mutation

   Silent mutation at Arg 132 (CGT to CGC), deleted …

   Promoter

   CMV

   Available Since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. MK08_HUMAN_D0

   Plasmid

   #79695

   Purpose

   This plasmid encodes the kinase domain of MK08. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   John Chodera, Nicholas Levinson, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   MK08 (MAPK8 Human)
   

   Tags

   His10-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

5. MDIS2/MRH1 P1_pECIA14

   Plasmid

   #114839

   Purpose

   Prey vector MDIS2/MRH1 P1_pECIA14 should be used with bait vector MDIS2/MRH1 P1_pECIA2.

   Depositor

   Youssef Belkhadir

   Article

   Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

   Insert

   AT4G18640 (MRH1 Mustard Weed)
   

   Expression

   Insect

   Promoter

   Metallothionein (Copper-Inducible)

   Available Since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMSCV40-EF1a-hBCL10(1-185)-SO

   Plasmid

   #75258

   Purpose

   retroviral expression vector for expression of StrepOne-Tagged human BCL10 (AS 1-185)

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   Bcl10 (BCL10 Human)
   

   Use

   Retroviral

   Tags

   StrepOne Tag

   Expression

   Mammalian

   Mutation

   AS 1-185 (w/o C-terminus)

   Promoter

   pMSCV-LTRs, EF1a

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMXS-IRES-BLAST flag-CAD M1601E

   Plasmid

   #169828

   Purpose

   Expresses C-terminal flag-tagged CAD with mutation at reported dimerization interface of the DHOase domain

   Depositor

   Richard Possemato

   Article

   Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

   Insert

   CAD (CAD Human)
   

   Use

   Retroviral

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   M1601E; TCCC -> AGTC silent mutations at nt527…

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. IL2-mCherry-AH in pmCherryN1

   Plasmid

   #187284

   Purpose

   Express IL2-AH-mCherry

   Depositor

   Alpha Yap

   Article

   Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

   Insert

   interleukin-2 receptor alpha-subunit, anillin (IL2, ANLN Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   AH

   Promoter

   CMV

   Available Since

   Sept. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. Venus-Bad-2A-pEGFP-C1

   Plasmid

   #166748

   Purpose

   Express Venus fused to the N-terminus of the Bcl-2 family protein, Bad with mutation to 2 positions in the BH3 domain

   Depositor

   David Andrews

   Article

   Liu et al Elife. 2019 Mar 12;8. pii: 37689. doi: 1

   Insert

   Bad-2A (BAD Human)
   

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   2 hydrophobic residues in the BH3 region mutated …

   Promoter

   CMV

   Available Since

   March 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. Venus-Puma-4E-pEGFP-C1

    Plasmid

    #166746

    Purpose

    Express Venus fused to the N-terminus of the Bcl-2 family protein, Puma with mutation in BH3 domain to disrupt binding anti-apoptotic proteins

    Depositor

    David Andrews

    Article

    Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

    Insert

    Puma-4E (BBC3 Human)
    

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    4 hydrophobic residues in the BH3 region mutated …

    Promoter

    CMV

    Available Since

    March 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. Venus-tBid-2E-pEGFP-C1

    Plasmid

    #166747

    Purpose

    Express Venus fused to the N-terminus of the Bcl-2 family protein, tBid with mutation to 2 positions in the BH3 domain

    Depositor

    David Andrews

    Article

    Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

    Insert

    tBid-2E (Bid Mouse)
    

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    2 hydrophobic residues in the BH3 region mutated …

    Promoter

    CMV

    Available Since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLuc-Optop38i-dsm

    Plasmid

    #89748

    Purpose

    Expression of light-regulated p38 inhibitor, firefly luciferase-fused, dark-state mutant photosensor (AsLov2Ja.C450A), inhibitor MK3BD3-13F

    Depositor

    Michael Courtney

    Article

    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

    Insert

    Optop38i dark-state mutant
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Mutation

    C450A mutation of AsLov2Jalpha

    Promoter

    CMV

    Available Since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEAHISPA4631

    Plasmid

    #97017

    Purpose

    Expressing Pseudomonas aeruginosa orf PA4631 protein

    Depositor

    Huanting Liu, James Naismith

    Article

    Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

    Insert

    Nucleoside-diphosphate-sugar epimerase
    

    Tags

    N-ter TEV protease cleavable 6HIs

    Mutation

    none

    Promoter

    T7

    Available Since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. CSKP_HUMAN_D0

    Plasmid

    #79714

    Purpose

    This plasmid encodes the kinase domain of CSKP. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Nicholas Levinson, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    CSKP (CASK Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pMSCV40-EF1a-hBCL10(1-114)-SO

    Plasmid

    #75257

    Purpose

    retroviral expression vector for expression of StrepOne-Tagged human BCL10 (AS 1-116)

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    Bcl10 (BCL10 Human)
    

    Use

    Retroviral

    Tags

    StrepOne Tag

    Expression

    Mammalian

    Mutation

    AS 1-116 (CARD-domain)

    Promoter

    pMSCV-LTRs, EF1a

    Available Since

    June 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLX307/PTPN11

    Plasmid

    #140941

    Purpose

    expresses PTPN11

    Depositor

    Kimberly Stegmaier

    Article

    Cremer et al Cancer Discov. 2020 Feb;10(2):214-231. d

    Insert

    PTPN11 protein tyrosine phosphatase non-receptor type 11 [ Homo sapiens (human) ] (PTPN11 Human)
    

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONR223-NME6

    Plasmid

    #23562

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    NME6 (NME6 Human)
    

    Use

    Gateway donor vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

18. pET30-2-GPD1

    Plasmid

    #83911

    Purpose

    stable overexpression

    Depositor

    David Sabatini

    Article

    Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

    Insert

    glycerol-3-phosphate dehydrogenase 1 (GPD1 Human)
    

    Tags

    6x His tags

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLPC-dnMAML1-mVenus

    Plasmid

    #171023

    Purpose

    Retroviral vector for CMV promoter driven expression of dominant negative (DN) MAML1 (Notch pathway) fused to mVenus fluorescent protein (to be used in conjunction with Phoenix packaging cells).

    Depositor

    Masashi Narita

    Article

    Hoare et al Nat Cell Biol. 2016 Sep;18(9):979-92. do

    Insert

    MAML1 Dominant Negative (MAML1 Human)
    

    Use

    Retroviral

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    amino acids 12-74 only, hence conferring dominant…

    Promoter

    CMV

    Available Since

    April 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-FLT3-D835Y-V5/HIS

    Plasmid

    #236007

    Purpose

    expression of the D835Y kinase defective mutant variant of human FLT3 that is associated with Acute Myeloid Leukemia and Acute Lymphoblastic Leukemia

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    human FLT3-D835Y receptor tyrosine kinase, full length (FLT3 Human)
    

    Tags

    V5/His

    Expression

    Mammalian

    Mutation

    D835Y substitution

    Promoter

    CMV

    Available Since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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