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pLenti-Blast-sgSIK1-D

Plasmid

#138680

Purpose

Expresses a mouse SIK1-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgSIK1 mouse (Sik1 Mouse)

Use

Lentiviral

Promoter

hU6

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-Blast-sgSIK1-B

Plasmid

#138679

Purpose

Expresses a mouse SIK1-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgSIK1 mouse (Sik1 Mouse)

Use

Lentiviral

Promoter

hU6

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pLPC-Flag-SOCS1-Y80D

Plasmid

#129516

Purpose

Retroviral vector for the expression of human SOCS1 with a Y80D mutation and a FLAG tag in N-terminal

Depositor

Gerardo Ferbeyre

Article

Saint-Germain et al Cancer Res. 2019 Jul 1;79(13):3306-3319.

Insert

SOCS1 (SOCS1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Changed Tyrosine 80 to Aspartic acid

Promoter

CMV

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pLPC-Flag-SOCS1-Y80F

Plasmid

#129517

Purpose

Retroviral vector for the expression of human SOCS1 with a Y80F mutation and a FLAG tag in N-terminal

Depositor

Gerardo Ferbeyre

Article

Saint-Germain et al Cancer Res. 2019 Jul 1;79(13):3306-3319.

Insert

SOCS1 (SOCS1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Changed Tyrosine 80 to Phenylalanine

Promoter

cmv

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pTBL405 CHYRON1 integration construct

Plasmid

#126442

Purpose

To integrate the CHYRON1 locus at HEK293site3.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Inserts

pU6-CHYRON1 hgRNA
pCMV-puro

Expression

Mammalian

Mutation

The SpCas9 sgRNA constant region is mutated to ma…

Promoter

CMV and human U6

Available Since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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pTagGFP2-N SIN1 Na

Plasmid

#124928

Purpose

For expression of a.a. 1-68 of SIN1-GFP

Depositor

Jin Chen

Article

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Insert

MAPKAP1 (MAPKAP1 Human)

Tags

TagGFP2

Expression

Mammalian

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pLentiCMV-Blast mLST8-Flag Mut1

Plasmid

#124916

Purpose

For stable expression sgRNA resistant Flag tagging mLST8 Q44E/N46L mutant

Depositor

Jin Chen

Article

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Insert

MLST8 (MLST8 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Insert ORF was sirently mutated to be resistent t…

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pLentiCMV-Blast mLST8-Flag Mut2

Plasmid

#124917

Purpose

For stable expression_ sgRNA resistant Flag tagging mLST8 W272F/W274F mutant

Depositor

Jin Chen

Article

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Insert

MLST8 (MLST8 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Insert ORF was sirently mutated to be resistent t…

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pLX311 DEDD

Plasmid

#117745

Purpose

Open reading frame vector encoding DEDD

Depositor

William Hahn

Article

Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

Insert

DEDD1 (DEDD Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 Dynamin1 T78R L84R T92R V118R

Plasmid

#112106

Purpose

Mammalian expression plasmid of GFP-tagged Dynamin protein.

Depositor

Justin Taraska

Article

Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

Insert

Dynamin1 (DNM1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

T78R L84R T92R V118R

Promoter

CMV

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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TAF9 gRNA (BRDN0001148557)

Plasmid

#76563

Purpose

3rd generation lentiviral gRNA plasmid targeting human TAF9

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TAF9 (TAF9 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TAF9 gRNA (BRDN0001146200)

Plasmid

#76564

Purpose

3rd generation lentiviral gRNA plasmid targeting human TAF9

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TAF9 (TAF9 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TAF9 gRNA (BRDN0001146426)

Plasmid

#76565

Purpose

3rd generation lentiviral gRNA plasmid targeting human TAF9

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TAF9 (TAF9 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TAF9 gRNA (BRDN0001146418)

Plasmid

#76566

Purpose

3rd generation lentiviral gRNA plasmid targeting human TAF9

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TAF9 (TAF9 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PAPSS2 gRNA (BRDN0001145342)

Plasmid

#76316

Purpose

3rd generation lentiviral gRNA plasmid targeting human PAPSS2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PAPSS2 (PAPSS2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PAPSS2 gRNA (BRDN0001147912)

Plasmid

#76317

Purpose

3rd generation lentiviral gRNA plasmid targeting human PAPSS2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PAPSS2 (PAPSS2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PAPSS2 gRNA (BRDN0001146774)

Plasmid

#76318

Purpose

3rd generation lentiviral gRNA plasmid targeting human PAPSS2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PAPSS2 (PAPSS2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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KHBD00050

Plasmid

#34212

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG11502 (svp Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pPB_TRE3G_scFV-sfGFP-Dnmt3a3lCD

Plasmid

#235576

Purpose

Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector Dnmt3a3L fused with scFV to programme DNA methylation

Depositor

Jamie Hackett

Article

Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

Insert

Dnmt3a-Dnmt3L (Dnmt3a, Dnmt3l Mouse)

Use

CRISPR

Available Since

April 29, 2025

Availability

Academic Institutions and Nonprofits only

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pDONR223-ZNF532

Plasmid

#88740

Purpose

Donor Vector containing ZNF532 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF532 (ZNF532 Human)

Use

Gateway donor vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only