Addgene

We narrowed to 24,257 results for: nans

Showing: 921 - 940 of 24257 results

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pTV-Nanog_2-5prime-1000-24MS96T-3F_NeoR

Plasmid

#174882

Purpose

Targeting vector for generating Nanog STREAMING-tag KI cell

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

STREAMING-tag with homology arms for Nanog

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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dCas9 fused to nanobody (anti-beta catenin)

Plasmid

#186420

Purpose

Expression of dCas9 with C-terminal nanobody fusion recognizing beta catenin tag

Depositor

Stephen Elledge

Article

Barber et al Cell Rep Methods. 2022 Oct 17;2(10):1003

Insert

dCas9-nanobody fusion (anti-beta catenin)

Use

CRISPR

Tags

6His

Expression

Mutation

Promoter

Available since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-amyloid-β 40/42 nanobody (R3VQ) with sortase tag

Plasmid

#233217

Purpose

Mammalian epression of anti-amyloid-β 40/42 nanobody (R3VQ) with a sortase tag for direct dye conjugation.

Depositor

Jeff Lichtman

Article

Han et al bioRxiv 2023.10.24.563674

Insert

Anti-amyloid-β 40/42 nanobody (R3VQ)

Use

Tags

Sortase tag, His tag

Expression

Mammalian

Mutation

Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-EGFP-anti-rabbit-IgG(Fc)-nanobody

Plasmid

#138133

Purpose

Expresses GST-tagged EGFP-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

Insert

EGFP-anti-rabbit-IgG(Fc)-nanobody

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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pCW-Tet-Cas9-miRFP670nano-PGK-Blast

Plasmid

#196722

Purpose

Tet-inducible expression of Cas9-T2A-miRFP670nano

Depositor

Bernhard Schmierer

Insert

Cas9-T2A-miRFP670nano

Use

CRISPR and Lentiviral

Tags

3xFLAG-SV40NLS and Nucleoplasmin NLS

Expression

Mutation

Promoter

TRE, PGK

Available since

April 12, 2023

Availability

Academic Institutions and Nonprofits only

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pSb_init containing the TM287/288 nanobody Nb_TM#2

Plasmid

#132698

Purpose

expresses the TM287/288 nanobody Nb_TM#2 in E.coli, to use as positive control in ELISA

Depositor

Markus Seeger

Article

Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

Insert

nanobody Nb_TM#2

Use

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-Flag3-pG-mNanog-pgk-hph

Plasmid

#74911

Purpose

Mammalian expression of Flag tagged mNanog

Depositor

Jose Silva

Article

Theunissen et al Development. 2011 Nov;138(22):4853-65. d

Insert

Nanog (Nanog Mouse)

Use

Tags

3xFlag

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-Klf2.2A.Nanog-Cherry-pA-pgk-zeo

Plasmid

#74919

Purpose

Mammalian expression of mKlf2 and mNanog-mCherry

Depositor

Jose Silva

Article

dos Santos et al Cell Stem Cell. 2014 Jul 3;15(1):102-10.

Inserts

Klf2 (Klf2 Mouse)
Nanog (Nanog Mouse)

Use

Tags

T2A and mCherry

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 26, 2016

Availability

Academic Institutions and Nonprofits only
C511-E02: attB5r-NanoLuc-nostop-attB1r

Plasmid

#162938

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal Nanoluciferase fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Nanoluciferase

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-mNanog-mut-pgk-hph

Plasmid

#74914

Purpose

Mammalian expression of mNanog (Y42E, K43T)

Depositor

Jose Silva

Article

Theunissen et al Development. 2011 Nov;138(22):4853-65. d

Insert

Nanog (Nanog Mouse)

Use

Tags

Expression

Mammalian

Mutation

K43T of Nanog protein and Y42E of Nanog homeodoma…

Promoter

CAG

Available since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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pB80-KIF1A(1-383)-GFP-SSPB(nano)

Plasmid

#174628

Purpose

Constitutively active KIF1A for optogenetic heterodimerization to iLID via SSPB(nano)

Depositor

Lukas Kapitein

Article

Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

KIF1A(1-383)-GFP-SSPB(nano) (Kif1a Mouse, Synthetic)

Use

Tags

GFP-SSPB(nano)

Expression

Mammalian

Mutation

mmKIF1A(aa1-383): Pro202Ala; EGFP: Met1Del;

Promoter

Chicken beta-actin

Available since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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Nuc-Orange Nano-lantern(Ca2+)/pcDNA3

Plasmid

#71349

Purpose

Express Nuc-Orange Nano-lantern-based luminescent Ca2+ indicator in mammalian cel

Depositor

Takeharu Nagai

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Orange Nano-lantern(Ca2+)_H2B

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 8, 2015

Availability

Academic Institutions and Nonprofits only
Mito-Cyan Nano-lantern(Ca2+)/pcDNA3

Plasmid

#71345

Purpose

Express Mito-Cyan Nano-lantern-based luminescent Ca2+ indicator in mammalian cel

Depositor

Takeharu Nagai

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

hOTC_Cyan Nano-lantern(Ca2+)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 8, 2015

Availability

Academic Institutions and Nonprofits only
pPB-CAG-Flag3-pG-cNanog-pgk-hph

Plasmid

#74912

Purpose

Mammalian expression of Flag tagged cNanog

Depositor

Jose Silva

Article

Theunissen et al Development. 2011 Nov;138(22):4853-65. d

Insert

Nanog

Use

Tags

3xFlag

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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TRE3G-mCherry-P2A-T2A-nanobody-KS-FtoA

Plasmid

#238288

Purpose

For integration of mCherry-P2A-T2A-nanobody-KS-FtoA

Depositor

Denes Hnisz

Insert

mCherry-P2A-T2A-nanobody-KS-FtoA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCSIIbleo-miRFP670nano(R57C/C86S)-P2A-EGFP

Plasmid

#197370

Purpose

The lentiviral vector for miRFP670nano(R57C/C86S) and EGFP

Depositor

Michiyuki Matsuda

Insert

miRFP670nano(R57C/C86S)

Use

Lentiviral

Tags

P2A-EGFP

Expression

Mammalian

Mutation

R57C/C86S mutation

Promoter

CAG

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-GST-DmNanos_116-163-V5His6_AC

Plasmid

#148469

Purpose

Insect Expression of DmNanos_116-163

Depositor

Elisa Izaurralde

Article

Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

Insert

DmNanos_116-163 (nos Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpG-DmNanos-opt_50-236-GB1opt_AB

Plasmid

#148331

Purpose

Bacterial Expression of DmNanos-opt_50-236

Depositor

Elisa Izaurralde

Article

Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

Insert

DmNanos-opt_50-236 (nos Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpG-DmNanos-opt_116-163-L127DF130D_AB

Plasmid

#148333

Purpose

Bacterial Expression of DmNanos-opt_116-163-L127DF130D

Depositor

Elisa Izaurralde

Article

Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

Insert

DmNanos-opt_116-163-L127DF130D (nos Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpG-DmNanos-opt_116-163-F152E_AB

Plasmid

#148332

Purpose

Bacterial Expression of DmNanos-opt_116-163-F152E

Depositor

Elisa Izaurralde

Article

Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

Insert

DmNanos-opt_116-163-F152E (nos Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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