We narrowed to 6,650 results for: tac

1. pGEX-KG-CAND1

   Plasmid

   #19949

   Depositor

   Yue Xiong

   Article

   Liu et al Mol Cell. 2002 Dec . 10(6):1511-8.

   Insert

   CAND1 (cullin-associated and neddylation-dissociated 1) (CAND1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   April 28, 2009

   Availability

   Academic Institutions and Nonprofits only

2. CL7_2xNLS_iGeoCas9(G)_2xNLS_His

   Plasmid

   #222989

   Purpose

   Bacterial expression of iGeoCas9(G) construct

   Depositor

   Jennifer Doudna

   Article

   Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

   Insert

   CL7_2xNLS_iGeoCas9(G)_2xNLS_His
   

   Use

   CRISPR

   Tags

   CL7 and His

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGEX2TK-Osh2-PH

   Plasmid

   #71370

   Purpose

   bacterial expression of PH domain for phosphoinositide binding assay

   Depositor

   Mark Lemmon

   Article

   Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

   Insert

   Osh2-PH
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Pleckstrin homology (PH) domain; aa 277-398

   Promoter

   tac

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pGEX2TK-Num1-PH

   Plasmid

   #71369

   Purpose

   bacterial expression of PH domain for phosphoinositide binding assay

   Depositor

   Mark Lemmon

   Article

   Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

   Insert

   Num1-PH
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Pleckstrin homology (PH) domain; aa2563-2692

   Promoter

   tac

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pGEX2TK-Skm1-PH

   Plasmid

   #71371

   Purpose

   bacterial expression of PH domain for phosphoinositide binding assay

   Depositor

   Mark Lemmon

   Article

   Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

   Insert

   Skm1-PH
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Pleckstrin homology (PH) domain; aa1-132

   Promoter

   tac

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pGEX-4T1-Cdc42 G15A

   Plasmid

   #69356

   Purpose

   expression of nucleotide free Cdc42 in bacterial cells

   Depositor

   Rafael Garcia-Mata

   Article

   Garcia-Mata et al Methods Enzymol. 2006;406:425-37.

   Insert

   Cdc42 (CDC42 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   G15A

   Promoter

   tac

   Available since

   Sept. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pSub-MBP-GFP(2M-IKQE)

   Plasmid

   #185757

   Purpose

   Bacterial expression of MBP-GFP(2M IKQE)

   Depositor

   Jeffrey Bode

   Article

   Akimoto et al ACS Cent Sci. 2022 Feb 23;8(2):275-281.

   Insert

   GFP(2M-IKQE)
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. GST-N1IC

   Plasmid

   #47612

   Purpose

   bacterial expression of GST-myc-Notch1 IC

   Depositor

   Urban Lendahl

   Article

   Beatus et al Development. 1999 Sep;126(17):3925-35.

   Insert

   Notch1 intracellular domain (Notch1 Mouse)
   

   Use

   Tags

   GST and myc

   Expression

   Bacterial

   Mutation

   contains amino acids 1753-2531

   Promoter

   tac

   Available since

   Aug. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

9. T7 promoter + rpsL reporter plasmid

   Plasmid

   #156455

   Purpose

   A mutational reporter plasmid that can gain Streptomycin and/or Tetracycline resistance upon mutation. Contains T7 promoter.

   Depositor

   Matthew D. Shoulders

   Article

   Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

   Inserts

   rpsL
   TcR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   ATG start codon mutated to ACG

   Promoter

   tac and tet

   Available since

   Oct. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pENV8(GAAA)-GFPuv

    Plasmid

    #70128

    Purpose

    Negative control cobalamin riboswitch reporter vector for use in E. coli; deletion of regulatory kissing loop interaction by conversion of L5 to GAAA tetra loop

    Depositor

    Robert Batey

    Article

    Johnson JE et al Nature. 2012 Dec 6;492(7427):133-7. doi:

    Insert

    kissing loop regulatory mutant of environmental cobalamin riboswitch sequence (env8)
    

    Use

    Synthetic Biology

    Tags

    None

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Nov. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. PGEX 4T-1-PPARgamma2

    Plasmid

    #78773

    Purpose

    To purify GST-tagged recombinant protein

    Depositor

    Jaewhan Song

    Article

    Kim et al Cell Death Differ. 2014 Apr;21(4):594-60

    Insert

    PPAR_2
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    June 10, 2016

    Availability

    Academic Institutions and Nonprofits only

12. GST eIF2beta

    Plasmid

    #45900

    Depositor

    Louise Larose

    Article

    Kebache et al Proc Natl Acad Sci U S A. 2002 Apr 16;99

    Insert

    eIF2S2 (Eif2s2 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

13. GST-CLCb

    Plasmid

    #47404

    Depositor

    Peter McPherson

    Article

    Legendre-Guillemin et al J Biol Chem. 2005 Feb 18;280(7):6101-8.

    Insert

    CLCb
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

14. GST-PRMT9 G260E mutant

    Plasmid

    #67610

    Purpose

    bacterial expression of GST-PRMT9 G260E Double E loop mutant

    Depositor

    Steven Clarke

    Article

    Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

    Insert

    PRMT9 (PRMT9 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    G260E double E loop mutant

    Promoter

    tac

    Available since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

15. GST-PRMT9 D258G mutant

    Plasmid

    #67609

    Purpose

    bacterial expression of GST-PRMT9 D258G Double E loop mutant

    Depositor

    Steven Clarke

    Article

    Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

    Insert

    PRMT9 (PRMT9 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    D258G double E loop mutant

    Promoter

    tac

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pFM111

    Plasmid

    #40157

    Depositor

    Geraldine Seydoux

    Article

    Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

    Insert

    par-1N:FLAG (par-1 Nematode)
    

    Use

    Tags

    Flag and GST

    Expression

    Bacterial

    Mutation

    Contains aa 1-964

    Promoter

    tac

    Available since

    Sept. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pFM112

    Plasmid

    #40158

    Depositor

    Geraldine Seydoux

    Article

    Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

    Insert

    par-1C:FLAG (par-1 Nematode)
    

    Use

    Tags

    GST and modified Flag

    Expression

    Bacterial

    Mutation

    Contains aa 966-1192

    Promoter

    tac

    Available since

    Oct. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

18. CL7_2xNLS_iGeoCas9(C2)_2xNLS_His

    Plasmid

    #222988

    Purpose

    Bacterial expression of iGeoCas9(C2) construct

    Depositor

    Jennifer Doudna

    Article

    Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

    Insert

    CL7_2xNLS_iGeoCas9(C2)_2xNLS_His
    

    Use

    CRISPR

    Tags

    CL7 and His

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. CL7_2xNLS_iGeoCas9(C1)_2xNLS_His

    Plasmid

    #222987

    Purpose

    Bacterial expression of iGeoCas9(C1) construct

    Depositor

    Jennifer Doudna

    Article

    Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

    Insert

    CL7_2xNLS_iGeoCas9(C1)_2xNLS_His
    

    Use

    CRISPR

    Tags

    CL7 and His

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLKO.1-shCdh2.1 GFP

    Plasmid

    #209099

    Purpose

    GFP expressing shRNA targeting Cdh2

    Depositor

    Cagla Eroglu

    Article

    Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

    Insert

    shCdh2.1
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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