We narrowed to 15,730 results for: NOL

1. TK1 gRNA (BRDN0001145069)

   Plasmid

   #76424

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TK1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   TK1 (TK1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-BE3-FNLS(RA)

   Plasmid

   #112671

   Purpose

   CMV expression vector for BE3-FNLS construct (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   BE3-FNLS
   

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   NLS sequence at the N-terminus and D10A

   Promoter

   CMV

   Available Since

   July 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. SARS_COV2_ORF6_HA_BASU

   Plasmid

   #153984

   Purpose

   Express Orf6 gene along with HA and BASU

   Depositor

   Paul Khavari

   Article

   Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

   Insert

   orf6 (ORF6 Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-hA3C-BE3

   Plasmid

   #113412

   Purpose

   Expresses hA3C-BE3 in mammalian cells

   Depositor

   Jia Chen

   Article

   Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

   Insert

   hA3C-BE3 (APOBEC3C S. pyogenes and Bacteriophage PBS2, Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMCB320-sgNC.SPA

   Plasmid

   #169834

   Purpose

   Expresses a negative control sgRNA

   Depositor

   Laura Attardi

   Article

   Bieging-Rolett et al Mol Cell. 2020 Nov 5;80(3):452-469.e9. d

   Insert

   safe harbor sgRNA
   

   Use

   CRISPR, Lentiviral, and Mouse Targeting

   Expression

   Mammalian

   Promoter

   mU6

   Available Since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR221_SLC39A8

   Plasmid

   #132177

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Polesel et al Biometals. 2022 Dec 1. doi: 10.1007/s105

   Insert

   SLC39A8 (SLC39A8 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 25, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pDisplay-GACh1.0

   Plasmid

   #106072

   Purpose

   express the genetically-encoded fluorescent ACh indicator GACh1.0 in mammalian cells

   Depositor

   Yulong Li

   Article

   Jing et al Nat Biotechnol. 2018 Sep;36(8):726-737.

   Insert

   GPCR activation based ACh sensor (GACh) 1.0
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMonAID_dCas9-PmCDA_Hyg_ALS

   Plasmid

   #91692

   Purpose

   Monocot Target-AID vector expressing rice-optimized dCas9-PmCDA1 with sgRNA targeting OsAls

   Depositor

   Akihiko Kondo

   Article

   Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   SpCas9
   

   Tags

   PmCDA1

   Expression

   Plant

   Mutation

   D10A and H840A for dead Cas9

   Promoter

   2x35S

   Available Since

   June 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. SARS_COV2_ORF8_HA_BASU

   Plasmid

   #153982

   Purpose

   Express Orf8 gene along with HA and BASU

   Depositor

   Paul Khavari

   Article

   Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

   Insert

   orf8 (ORF8 Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYPQ265F2

    Plasmid

    #164722

    Purpose

    Gateway entry clone (attL1 & attR5) A3A/Y130F-nCas9-NG-CBE_V01 for C-T base editing

    Depositor

    Yiping Qi

    Article

    Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

    Insert

    A3A(Y130F)-zCas9(D10A)NG-UGI
    

    Use

    CRISPR; Gateway compatible a3a/y130f-ncas9-ng-cbe…

    Expression

    Plant

    Available Since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. SARS_COV2_nsp14_HA_BASU

    Plasmid

    #153993

    Purpose

    Express NSP14 gene along with HA and BASU

    Depositor

    Paul Khavari

    Article

    Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

    Insert

    NSP14 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. NUAK1 gRNA (BRDN0001162598)

    Plasmid

    #75577

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human NUAK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    NUAK1 (NUAK1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-ACBEmax

    Plasmid

    #139106

    Purpose

    Mammalian ACBEmax expression vector (pSI-632)

    Depositor

    Nozomu Yachie

    Article

    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    Bi_partite_NLS-TadA*-XTEN_linker-TadA-XTEN_linker-SpCas9(D10A)-SH3_linker-rAPOBEC1-2xUGI-SV40NLS
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-dCpf1-BE-YEE

    Plasmid

    #107687

    Purpose

    Expresses dLbCpf1-BE-YEE in mammalian cells

    Depositor

    Jia Chen

    Article

    Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    dLbCpf1-BE-YEE
    

    Expression

    Mammalian

    Mutation

    APOBEC1(W90Y/R126E/R132E), dLbCpf1(D832A/E925A/D1…

    Promoter

    CMV

    Available Since

    April 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. Polymerase expression - pCMV-Phi29(+exo) (LM2759)

    Plasmid

    #208964

    Purpose

    Unfused Phi29 DNA polymerase (+exo), expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    Phi29(+exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. AA145

    Plasmid

    #215937

    Purpose

    Fragmid fragment: (guide cassette) guide expression; Chen F+E trRNA (preferred)

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    U6_v1; BsmBI_v0; BsmBI_v1; trRNA_v4 [Sp]
    

    Use

    CRISPR; Fragment

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV_eA3A T31A-dCas9

    Plasmid

    #163537

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    eA3A T31A-dCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    dCas9 (D10A, H840A)eA3A T31A

    Available Since

    Dec. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. NUAK1 gRNA (BRDN0001147733)

    Plasmid

    #75576

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human NUAK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    NUAK1 (NUAK1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. NUAK1 gRNA (BRDN0001162525)

    Plasmid

    #75578

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human NUAK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    NUAK1 (NUAK1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. ABE8.17-d

    Plasmid

    #136299

    Purpose

    expresses ABE8.17-d in mammalian cells

    Depositor

    Nicole Gaudelli

    Article

    Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

    Insert

    ABE8.20-d
    

    Tags

    C-terminal BPNLS

    Expression

    Mammalian

    Mutation

    D10A in S. pyogenes Cas9, TadA mutations describe…

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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