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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,410 results for: TIM;

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Showing: 9441 - 9460 of 14410 results

  3. pET28a(+)v2_TwinStrep-SUMO-FLP-8xHis

    Plasmid
    #246980
    Purpose
    Expresses E. coli codon-optimized TwinStrep-SUMO-tagged FLP recombinase with N- and C-terminal NLS, using an improved pET design from Shilling et al. 2020.
    Depositor
    Amro Hamdoun
    Insert
    FLP Recombinase
    Use
    Synthetic Biology
    Tags
    8xHis, Nucleoplasmin, SUMO, SV40, and TwinStrep
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Nov. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    246980-plasmid-map-sequence-id-491526

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  4. pT7CFE1_polio_3ABC_C147R

    Plasmid
    #212985
    Purpose
    Encodes Poliovirus type I Mahoney 3ABC with active site mutation optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    Poliovirus Type I Mahoney 3ABC C147R
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Mutation
    cysteine 147 in 3C changed to arginine
    Promoter
    T7
    Available Since
    Feb. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    212985-plasmid-map-sequence-id-422575

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  5. pT7CFE1_EVA71_VP12A_C110A

    Plasmid
    #212976
    Purpose
    Encodes EV-A71 4643 VP12A with active site mutation optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    Enterovirus A71 4643 VP12A C110A
    Use
    Mammalian cell free protein expression
    Tags
    HA tag
    Mutation
    changed cysteine 110 from 2A to alanine
    Promoter
    T7
    Available Since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212976-plasmid-map-sequence-id-420360

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  6. pT7CFE1_polio_VP12A_C109R

    Plasmid
    #212987
    Purpose
    Encodes Poliovirus type I Mahoney VP12A with active site mutation optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    Poliovirus Type I Mahoney VP12A C109R
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Mutation
    Cysteine 109 from 2A mutated to arginine
    Promoter
    T7
    Available Since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212987-plasmid-map-sequence-id-420359

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  12. psubCMV-WPRE-IL3SP-mVEGF-D-dNdC

    Plasmid
    #119229
    Purpose
    Plasmid encodes proteolytically cleaved murine VEGF-D deltaN deltaC form, which has signal peptide from murine IL3 gene to be used for stimulation of lymphangiogenesis in mice in vivo.
    Depositor
    Kari Alitalo
    Insert
    IL3SP-Vegfd-dNdC (Vegfd Mouse)
    Use
    AAV
    Promoter
    CMV
    Available Since
    March 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    119229-plasmid-map-sequence-id-239316

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  14. pHH0103 UTROPHIN WW domain

    Plasmid
    #104275
    Purpose
    Bacterial expression of WW domain from UTROPHIN
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    UTROPHIN WW domain (UTRN Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104275-plasmid-map-sequence-id-203220

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  15. pHH0103 KIAA1688 WW domain #1

    Plasmid
    #104277
    Purpose
    Bacterial expression of WW domain #1 from KIAA1688
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    KIAA1688 WW domain #1 (ARHGAP39 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104277-plasmid-map-sequence-id-202577

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  16. pHH0103 KIAA1688 WW domain #2

    Plasmid
    #104278
    Purpose
    Bacterial expression of WW domain #2 from KIAA1688
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    KIAA1688 WW domain #2 (ARHGAP39 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104278-plasmid-map-sequence-id-203477

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  17. pHH0103 IQGAP2 WW domain

    Plasmid
    #104280
    Purpose
    Bacterial expression of WW domain from IQGAP2
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    IQGAP2 WW domain (IQGAP2 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104280-plasmid-map-sequence-id-203486

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  18. pHH0103 ARHGAP27 WW domain #1

    Plasmid
    #104282
    Purpose
    Bacterial expression of WW domain #1 from ARHGAP27
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    ARHGAP27 WW domain #1 (ARHGAP27 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104282-plasmid-map-sequence-id-203481

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  19. pHH0103 ARHGAP27 WW domain #2

    Plasmid
    #104283
    Purpose
    Bacterial expression of WW domain #2 from ARHGAP27
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    ARHGAP27 WW domain #2 (ARHGAP27 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104283-plasmid-map-sequence-id-203211

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  20. pHH0103 PDZK10 WW domain

    Plasmid
    #104285
    Purpose
    Bacterial expression of WW domain from PDZK10
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    PDZK10 WW domain (FRMPD4 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104285-plasmid-map-sequence-id-202571

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Showing: 9441 - 9460 of 14410 results