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pLenti6.3-SauriCas9-BlastR

Plasmid

#220967

Purpose

Expresses human codon-optimized SauriCas9

Depositor

Hyongbum Kim

Article

Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

Insert

SauriCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pRcCMV Cep164 (Nigg CW325)

Plasmid

#41148

Depositor

Erich Nigg

Article

Graser et al J Cell Biol. 2007 Oct 22;179(2):321-30.

Insert

Cep164 (CEP164 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

June 7, 2013

Availability

Academic Institutions and Nonprofits only
pMSCV-IRES-mOrange

Plasmid

#214807

Purpose

Mammalian expression vector

Depositor

Norio Komatsu

Article

De Marchi et al Leuk Res. 2022 Aug;119:106883. doi: 10.1

Type

Empty backbone

Use

Retroviral

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pFX-H2B-iRFP

Plasmid

#175842

Purpose

Express iRFP with nucleus-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

iRFP with nucleus-targeting sequence

Tags

H2B (human histone 2B)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV3Tag8li-hSAPS1-HA

Plasmid

#165466

Purpose

Human SAPS1 mammalian expression vector with C-terminal HA tag.

Depositor

Martin Dorf

Article

Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

Insert

Protein phosphatase 6 regulatory subunit 1 (PPP6R1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-mGold-Rab4a-C-7

Plasmid

#158002

Purpose

Visualization of endosome in mammalian cells.

Depositor

Francois St-Pierre

Article

Lee et al Sci Adv. 2020 Oct 23;6(43). pii: 6/43/ea

Insert

Rab4a (RAB4A Human)

Tags

mGold

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-mROCK1-delta2

Plasmid

#101290

Purpose

Mammalian expression of Rock1-GFP for the study of localisation signaling.

Depositor

Alpha Yap

Article

Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

Insert

Rock1 (Rock1 Mouse)

Expression

Mammalian

Mutation

1-727 aa

Promoter

CMV

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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mRFP-LAP2β

Plasmid

#228029

Purpose

lentiviral construct for mRFP-LAP2β expression

Depositor

David Pellman

Article

Zhao et al Dev Cell. 2023 May 22;58(10):847-865.e10

Insert

LAP2b (Tmpo Rat)

Use

Lentiviral

Tags

mRFP

Expression

Mammalian

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-mROCK1-delta3

Plasmid

#101291

Purpose

Mammalian expression of Rock1-GFP for the study of localisation signaling.

Depositor

Alpha Yap

Article

Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

Insert

Rock1 (Rock1 Mouse)

Expression

Mammalian

Mutation

1-1080 aa

Promoter

CMV

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pFX-GalT-EGFP

Plasmid

#174471

Purpose

Express EGFP with golgi apparatus-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

EGFP with golgi apparatus-targeting sequence

Tags

β1,4-galactose transferase

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pC002 - LshC2C2 locus into pACYC184

Plasmid

#79151

Purpose

Endogenous LshC2c2 locus cloned into pACYC184

Depositor

Feng Zhang

Article

Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

Insert

LshC2c2 locus

Expression

Bacterial

Available Since

June 30, 2016

Availability

Academic Institutions and Nonprofits only

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pSUMO-LIC-CHIKV-nsP2h

Plasmid

#127935

Purpose

Expresses CHIKV nsP2h (1-465 aa) in bacterial cells

Depositor

Dahai Luo

Article

Law et al Proc Natl Acad Sci U S A. 2019 May 7;116

Insert

CHIKV nsP2h

Tags

His6-Sumo-TEV

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

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TagRFP-T-Rabenosyn-5

Plasmid

#37537

Depositor

Silvia Corvera

Article

Navaroli et al Proc Natl Acad Sci U S A. 2012 Feb 21;10

Insert

Rabenosyn5 (RBSN Human)

Tags

TagRFP-T

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pAGM35171

Plasmid

#153222

Purpose

Level 1 position 1 part containing the Nos promoter, the BAR gene, and the Ocs terminator

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Insert

Nos promoter, BAR gene, Ocs terminator

Use

Synthetic Biology

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pSW457 - tCLV3

Plasmid

#115980

Purpose

entry vector for GreenGate cloning method, terminator

Depositor

Jan Lohmann

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

tCLV3

Use

Unspecified

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pSW455 - pCLV3

Plasmid

#115979

Purpose

entry vector for GreenGate cloning method, promoter

Depositor

Jan Lohmann

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

pCLV3

Use

Unspecified

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-mROCK1-delta1

Plasmid

#101289

Purpose

Mammalian expression of Rock1-GFP for the study of localisation signaling.

Depositor

Alpha Yap

Article

Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

Insert

Rock1 (Rock1 Mouse)

Expression

Mammalian

Mutation

1-375 aa

Promoter

CMV

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pCI HA NEDD4L S382A S468A

Plasmid

#27003

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

NEDD4L (NEDD4L Human)

Tags

HA

Expression

Mammalian

Mutation

S382A S468A

Available Since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only
pSLQ1371-sgGAL4

Plasmid

#121514

Purpose

sgGAL4 control. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.

Depositor

Ovijit Chaudhuri, Stanley Qi

Article

Lee et al Nat Commun. 2019 Apr 23;10(1):1848. doi:

Insert

sgGAL4

Use

CRISPR and Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

mouse U6

Available Since

April 22, 2019

Availability

Academic Institutions and Nonprofits only

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pAdv/TERT

Plasmid

#26744

Depositor

Bingliang Fang

Article

Gu et al Cancer Res. 2000 Oct 1. 60(19):5359-64.

Insert

human TERT promoter (TERT Human)

Expression

Mammalian

Available Since

March 15, 2011

Availability

Academic Institutions and Nonprofits only

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