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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 15,009 results for: flag

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Showing: 9441 - 9460 of 15009 results

  3. pMV7 lenti pEF-rTetR(SE-G72P)-3XFLAG-CACO1(Zn-C2H2_12)-T2A-mCherry-BSD-WPRE

    Plasmid
    #188753
    Purpose
    Lentiviral backbone containing CACO1 Zn-C2H2_12 domain as a fusion with rTetR(SE-G72P)-3XFLAG
    Depositor
    Michael Bassik, Lacra Bintu
    Publication
    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038
    Insert
    CACO1 Zn-C2H2_12 (CALCOCO1 Human)
    Use
    Lentiviral
    Tags
    rTetR(SE-G72P)-3xFLAG
    Expression
    Mammalian
    Promoter
    pEF1a
    Available since
    May 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    188753-plasmid-map-sequence-id-371427

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  7. pCLAP

    Plasmid
    #200786
    Purpose
    Cloning template for N-terminal CLIPf tagging and C-terminal SNAPf tagging of constructs for mammalian expression. Also used for the mammalian expression of CLIPf-SNAPf-HisTag-FLAG fusion protein.
    Depositor
    Alison Twelvetrees
    Publication
    Smith et al iScience. 2026 Feb 2;29(3):114875. doi:
    Type
    Empty backbone
    Tags
    CLIPf and SNAPf-6xHis-FLAG
    Expression
    Mammalian
    Available since
    Dec. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    200786-plasmid-map-sequence-id-435692

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  8. SpCas9-NRTH

    Plasmid
    #198541
    Purpose
    Expresses human codon-optimized SpCas9-NRTH and blasticidin resistance: EFS promoter-SpCas9-NRTH-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-NRTH
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198541-plasmid-map-sequence-id-388886

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  9. SpCas9-NRCH

    Plasmid
    #198542
    Purpose
    Expresses human codon-optimized SpCas9-NRCH and blasticidin resistance: EFS promoter-SpCas9-NRCH-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-NRCH
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198542-plasmid-map-sequence-id-388906

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  10. SpG

    Plasmid
    #198543
    Purpose
    Expresses human codon-optimized SpG and blasticidin resistance: EFS promoter-SpG-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpG
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    198543-plasmid-map-sequence-id-388887

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  11. pCAG-OSF-PP-ULK1(MIT)2, residues 833-1050

    Plasmid
    #180644
    Purpose
    Mammalian expression vector for ULK1 MIT domains, residues 833-1050. Has Precision protease cleavable N-terminal One-Strep Flag tag. Internal ID: WISP20-37.
    Depositor
    Wesley Sundquist
    Publication
    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75
    Insert
    ULK1 (ULK1 Human)
    Tags
    One-Strep-Flag (OSF)
    Expression
    Mammalian
    Mutation
    Residues 833-1050
    Promoter
    pCAG
    Available since
    July 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    180644-plasmid-map-sequence-id-358523

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  16. pcDNA5-FRT-TO-FH-Nsp15

    Plasmid
    #157721
    Purpose
    mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp15 under control of a tetracycline-inducible promoter
    Depositor
    David Tollervey
    Publication
    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FH-Nsp15 (ORF1ab SARS-CoV-2)
    Tags
    Flag-His8
    Expression
    Mammalian
    Mutation
    codon optimized
    Available since
    Aug. 10, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    157721-plasmid-map-sequence-id-308142

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  18. pcDNA5-FRT-TO-FH-Nsp8

    Plasmid
    #157695
    Purpose
    mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoter
    Depositor
    David Tollervey
    Publication
    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FH-Nsp8 (ORF1ab SARS-CoV-2)
    Tags
    Flag-His8
    Expression
    Mammalian
    Mutation
    codon optimized
    Available since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    157695-plasmid-map-sequence-id-307838

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  19. pcDNA5-FRT-TO-FH-Nsp7

    Plasmid
    #157689
    Purpose
    mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp7 under control of a tetracycline-inducible promoter
    Depositor
    David Tollervey
    Publication
    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FH-Nsp7 (ORF1ab SARS-CoV-2)
    Tags
    Flag-His8
    Expression
    Mammalian
    Mutation
    codon optimized
    Available since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    157689-plasmid-map-sequence-id-307827

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  20. pCS-TRE-FHDnd1-Y102A-Ubc-rtTA-I2G

    Plasmid
    #70075
    Purpose
    2nd gen lentiviral vector. tet/dox controllable expression of FLAG-HA-tagged murine Dnd1 (RRM mutant) in mammalian cells
    Depositor
    Thomas Tuschl
    Publication
    Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d
    Insert
    Dnd1 (Dnd1 Mouse)
    Use
    Lentiviral
    Tags
    FLAG and HA
    Expression
    Mammalian
    Mutation
    Y102A. Silent mutations are introduced to escape …
    Promoter
    Tet responsible promoter
    Available since
    Nov. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    70075-plasmid-map-sequence-id-129458

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Showing: 9441 - 9460 of 15009 results