We narrowed to 26,912 results for: Emb
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Plasmid#116853PurposePI(3,4)P2 biosensorDepositorInsertPLEKHA1 (PLEKHA1 )
TagsEGFP and X.leavis map2k1.L(32-44)ExpressionMammalianMutationAmino acids 169.329Available SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMAL-SH3(2)-5R
Plasmid#112089PurposeBacterial expression plasmid containing His and MBP tags for 5 repeats of the second SH3 domain from human NCK1.DepositorInsertSH3(2)-5R (NCK1 Human)
TagsHis6 and MBPExpressionBacterialMutationconstruct contains only repeats of the second SH3…PromoterLacAvailable SinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
mEos3-Rac1
Plasmid#92039PurposeExpresses mEos3-Rac1 fusion proteinDepositorAvailable SinceJune 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Delta(Pro-MP)ADAM10-HA
Plasmid#65107PurposeExpression of HA tagged ADAM10 lacking prodomain and metalloprotease domain in mammalian cellsDepositorInsertDelta(Pro-MP)ADAM10 (ADAM10 Human)
TagsHAExpressionMammalianMutationdeleted AA 19-455PromoterCMVAvailable SinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
mEos3-dnRac1
Plasmid#92040PurposeExpresses mEos3 fusion with dnRac1 (T17N).DepositorInsertRAC1 (RAC1 Human)
TagsmEos3ExpressionBacterial, Insect, and Mamm…MutationT17N (dominant negative)PromoterCMVAvailable SinceJune 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
mEos3-caRac1
Plasmid#92041PurposeExpresses mEos3 fusion with caRac1 (Q61L)DepositorInsertRAC1 (RAC1 Human)
TagsmEos3ExpressionBacterial, Insect, and Mamm…MutationQ61L (constitutively active)PromoterCMVAvailable SinceJune 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
OPA1-isoform7
Plasmid#70179PurposeMammalian expression of hsOPA1 isoform 7DepositorAvailable SinceFeb. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CMV-mCherry-hPMCA2w/b
Plasmid#111570PurposeExpresses a plasma membrane calcium pump hPMCA2w/bDepositorAvailable SinceSept. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLpA-DN.JNK1
Plasmid#51942PurposeAdenoviral vector for dominant negative JNK1 expressionDepositorInsertDN JNK1 (Mapk8 Mouse)
UseAdenoviralTagsFLAGMutationdominant negative: the dual phosphorylation moti…PromoterCMVAvailable SinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
Actin-mRFP-PAGFP
Plasmid#62382PurposeExpresses beta actin with both a PAGFP and mRFP tag.DepositorAvailable SinceNov. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL3-E-cadherin promoter
Plasmid#61798PurposeLuciferase reporter containing the mouse E-cadherin promoterDepositorInsert-178/+92 fragment of the mouse Cdh1 promoter (Cdh1 Mouse)
UseLuciferaseExpressionMammalianAvailable SinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
CaV1.3e[8a,11,31b,Δ32,42a]
Plasmid#49333PurposeRepaired Addgene #26576 to agree with the rat ref sequence. Repairs are @: nt 3310 (GTG->GCG) [Val->Ala]; nt 731 (TCA->GGA) [Ser->Gly].DepositorInsertCACNA1D (Cacna1d Rat)
ExpressionMammalianMutationContains exons 8a, 11, 31b and 42a.Deletion of ex…PromoterCMVAvailable SinceFeb. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
TMED4-bio-His
Plasmid#51854PurposeExpresses full-length Transmembrane emp24 domain-containing protein 4 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertTMED4 (TMED4 Human)
TagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianPromoterCMVAvailable SinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
HSC1-HS4-D4Z4c-GiP
Plasmid#58541PurposeDerived from HSC1-GiP where 2 copies of the HS4 insulator core inside the 3'LTR and the D4Z4-c insulator, are combined upstream of the EF1 alpha promoterDepositorInsertsEnhanced Green Fluorescence Protein, Puromycin resistance gene
D4Z4 repeat, partial
UseRetroviralExpressionMammalianPromoterHuman EF1-alphaAvailable SinceSept. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEVA1
Plasmid#58953PurposeContains a synthetic DNA sequence, VAR1u, that specifies the Var1 protein. The Cox4 targeting signal is fused to the N-terminus to direct import of Var1. Complements var1 mutations in mtDNA.DepositorInsertVAR1u, VAR1 recoded for expression from the nucleus
UseYeast ecoli shuttle vectorTagspreCox4 mitochondrial matrix targeting signalExpressionYeastMutationSynthetic gene in standard genetic codePromoterADH1Available SinceAug. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
ACE2(-1119)-luc
Plasmid#31110PurposeLuciferase reporter construct for human ACE2 promoterDepositorInsertACE2 promoter (ACE2 Human)
UseLuciferaseExpressionMammalianMutationpromoter region -1119 to +103Available SinceOct. 14, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHR-CMV-TetO2_3C-Avi-His6_IRES-HA-BirA-ER
Plasmid#113898PurposeMammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of HA-tagged ER-resident E. coli biotin ligaseDepositorTypeEmpty backboneUseLentiviralTags3C-Avi-His6PromoterCMV-MIE-TetO2Available SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
Il12b 400bp pro-Luc
Plasmid#20020DepositorAvailable SinceJan. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
Jag1-HA pIRES
Plasmid#17336DepositorAvailable SinceApril 24, 2008AvailabilityAcademic Institutions and Nonprofits only -
pIRES2-hPAC-FLAG
Plasmid#129494PurposeExpresses human TMEM206 tagged at the C-terminus with FLAGDepositorInserthuman PAC tagged with FLAG tag at the C-terminal end (PACC1 Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable SinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only