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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 13,244 results for: BASE

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Showing: 9481 - 9500 of 13244 results

  1. PRPF4.001.169.S34E

    Plasmid
    #137643
    Purpose
    Expresses N-terminal (His)6-TEV cleavage site fusion of human gene or portion of gene, with point mutation, in bacterial strains. pET based vector.
    Depositor
    Tara Davis
    Insert
    PRPF4 (PRPF4 Human)
    Tags
    (His)6-TEV
    Expression
    Bacterial
    Mutation
    001-169-S34E
    Available Since
    Jan. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    137643-plasmid-map-sequence-id-381376

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  2. PRPF4.211.522.gst

    Plasmid
    #137650
    Purpose
    Expresses N-terminal GST-(His)6-Thrombin cleavage site fusion of human gene or portion of gene in bacterial strains. pET based vector.
    Depositor
    Tara Davis
    Insert
    PRPF4 (PRPF4 Human)
    Tags
    GST-(His)6-thrombin
    Expression
    Bacterial
    Mutation
    211-522-gst
    Available Since
    Nov. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    137650-plasmid-map-sequence-id-377729

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  3. pSBbi-Pur-LPAR

    Plasmid
    #125205
    Purpose
    SB-transposon plasmid for stable expression of lipid raft-targeted FRET-based kinase activity reporter with EV spacer, with AarI cassette for cloning of a kinase-target peptide
    Depositor
    Eric Davis
    Insert
    Lyn-Phospho-Activity-Reporter, Empty
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    EF1a/RPBSA
    Available Since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    125205-plasmid-map-sequence-id-243607

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  8. pEF1a-PEmax-P2A-GFP

    Plasmid
    #202000
    Purpose
    Based on the pCMV-PEmax-P2A-GFP, we replaced the CMP promoter with the EF1a promoter for expression in iPSC.
    Depositor
    Daniel Geschwind
    Insert
    PEmax-P2A-GFP
    Use
    CRISPR and Synthetic Biology
    Tags
    GFP
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    202000-plasmid-map-sequence-id-395506

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  9. pTF268 (pFA6a-TEV-6xGly-3xFlag-KanMX)

    Plasmid
    #44095
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    3xFlag, 6xGly linker, ADH1 transcription terminat…
    Available Since
    April 10, 2013
    Availability
    Academic Institutions and Nonprofits only
    44095-plasmid-map-sequence-id-61672

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  10. piggyBAC-BRD4-Flag

    Plasmid
    #214432
    Purpose
    A piggyBAC based plasmid for the stable expression of BRD4 with a flag tag.
    Depositor
    Shaun Cowley
    Insert
    Brd4 bromodomain containing 4 (Brd4 Mouse)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    EF1
    Available Since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    214432-plasmid-map-sequence-id-437159

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  11. pOpen-RNAse A

    Plasmid
    #165565
    Purpose
    Ribonuclease A or RNase A; endoribonuclease purified from bovine pancreas. Important enzyme for the removal of RNA for RNA free DNA purification reactions such as plasmid DNA purification and genomic DNA purification, RNA removal from recombinant protein preparations, ribonuclease protection assays, mapping single-base mutations in DNA/RNA.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    RNAse A
    Use
    Synthetic Biology
    Available Since
    Aug. 17, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165565-plasmid-map-sequence-id-340093

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  13. zcUAS:PSD95.FingR-GFP-ZFC(CCR5TC)-KRAB(A)

    Plasmid
    #72638
    Purpose
    Tol2 based UAS modified with a CCR5TC binding site upstream of it, driving PSD95-FingR fused to GFP, CCR5TC zinc finger, and KRAB(A) repressor domain
    Depositor
    Joshua Bonkowsky
    Insert
    UAS upstream of PSD95-FingR with ZFC(CCR5TC) and KRAB(A)
    Use
    Gateway
    Available Since
    March 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    72638-plasmid-map-sequence-id-445453

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  14. pTF272 (pFA6a-TEV-6xGly-3xFlag-HphMX)

    Plasmid
    #44083
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    3xFlag, 6xGly linker, ADH1 transcription terminat…
    Available Since
    April 3, 2013
    Availability
    Academic Institutions and Nonprofits only
    44083-plasmid-map-sequence-id-61676

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  15. pOpen-Exonuclease III E. coli

    Plasmid
    #165571
    Purpose
    Double-stranded DNA specific exonuclease. Initiates at the 3' termini of linear double-stranded DNA with 5' overhangs or blunt ends and 3' overhangs containing less than four bases. Initiates at nicked sites in double-stranded DNA.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Exonuclease III E. coli
    Use
    Synthetic Biology
    Available Since
    Aug. 24, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165571-plasmid-map-sequence-id-340929

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  16. pActin5c-CD-dCas9m4-UDI-attB

    Plasmid
    #104879
    Purpose
    To generate point mutations using CRISPR/Cas9 system; encoded cytidine deaminase fused to deactived Cas9
    Depositor
    Christopher Potter
    Insert
    CD-dCas9(m4)-UDI
    Use
    CRISPR
    Expression
    Insect
    Available Since
    May 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    104879-plasmid-map-sequence-id-209447

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  18. pTF270 (pFA6a-PSc-6xGly-3xFlag-KanMX)

    Plasmid
    #44089
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    3xFlag, 6xGly linker, ADH1 transcription terminat…
    Available Since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44089-plasmid-map-sequence-id-61674

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  19. pZC03 (pFA6a-TEV-6xGly-V5-His3MX)

    Plasmid
    #44072
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, His3…
    Available Since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44072-plasmid-map-sequence-id-61692

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  20. AAV-CAG-K-red-SPOTIT

    Plasmid
    #191490
    Purpose
    Red fluorescent-based opioid sensor for the kappa opioid receptor in AAV viral vector under a CAG promoter
    Depositor
    Wenjing Wang
    Insert
    K-red-SPOTIT
    Use
    AAV
    Promoter
    CAG
    Available Since
    April 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    191490-plasmid-map-sequence-id-377865

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Showing: 9481 - 9500 of 13244 results