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pMx/ATP8A1-HA

Plasmid

#209225

Purpose

Mammalian expression of ATP8A1

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

Insert

ATP8A1 (ATP8A1 Human)

Use

Retroviral

Tags

HA

Available Since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pL0_CE-OriT

Plasmid

#202150

Purpose

Level 0 plasmid containing an origin of transfer sequence from pPtPBR11 required for making plasmids mobilizable for conjugation with CE overhangs used to build level 1 constructs.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Origin of transfer

Use

Synthetic Biology

Mutation

OriT sequence mutated to remove sapI sites domest…

Available Since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-ef1a-DIO-eGCaMP(plus)

Plasmid

#201149

Purpose

AAV virus production for Cre recombinase dependent expression of eGCaMP(plus)

Depositor

Andre Berndt

Article

Wait et al Res Sq. 2023 Aug 7:rs.3.rs-3146778. doi:

Insert

eGCaMP+

Use

AAV, Cre/Lox, and Mouse Targeting

Expression

Mammalian

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot3-F672AY676AF727A_S

Plasmid

#147505

Purpose

Mammalian Expression of HsNot3-F672AY676AF727A

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot3-F672AY676AF727A (CNOT3 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsNot2-W507E_S

Plasmid

#147510

Purpose

Mammalian Expression of HsNot2-W507E

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot2-W507E (CNOT2 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsNot2_2-429_S

Plasmid

#147485

Purpose

Mammalian Expression of HsNot2_2-429

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot2_2-429 (CNOT2 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsNot2_429-540_S

Plasmid

#147486

Purpose

Mammalian Expression of HsNot2_429-540

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot2_429-540 (CNOT2 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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SEC7-2Katushka-ter-Hygromycin

Plasmid

#184772

Purpose

Red marker for yeast late Golgi/early endosome. Integration of 2xKatushka tag at SEC7 C terminus. Uses antibiotic resistance marker hphMX6.

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

SEC7 (SEC7 Budding Yeast)

Tags

2Katushka

Expression

Yeast

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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KBB382

Plasmid

#185085

Purpose

HA-tagged POM152 with glycosylation sites mutated

Depositor

Ken Belanger

Article

Belanger et al Genetics. 2005 Nov;171(3):935-47. doi: 1

Insert

POM152

Tags

HA

Expression

Yeast

Mutation

Pom152 N280A, N398A, N???A, N???A = Pom152Dglyc

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only
pBN086

Plasmid

#173448

Purpose

express E. coli E69 (O9a:K30) Wzc_K540M with Y715E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc_K540M with Y715E

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

K540M, Y715E

Promoter

araBAD

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBN087

Plasmid

#173449

Purpose

express E. coli E69 (O9a:K30) Wzc_K540M with Y717E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc_K540M with Y717E

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

K540M, Y717E

Promoter

araBAD

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBN088

Plasmid

#173450

Purpose

express E. coli E69 (O9a:K30) Wzc_K540M with Y718E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc_K540M with Y718E

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

K540M, Y718E

Promoter

araBAD

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBN081

Plasmid

#173443

Purpose

express E. coli E69 (O9a:K30) Wzc_K540M with Y705E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc_K540M with Y705E

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

K540M, Y705E

Promoter

araBAD

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBN082

Plasmid

#173444

Purpose

express E. coli E69 (O9a:K30) Wzc_K540M with Y706E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc_K540M with Y706E

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

K540M, Y706E

Promoter

araBAD

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBN084

Plasmid

#173446

Purpose

express E. coli E69 (O9a:K30) Wzc_K540M with Y708E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc_K540M with Y708E

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

K540M, Y708E

Promoter

araBAD

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBN085

Plasmid

#173447

Purpose

express E. coli E69 (O9a:K30) Wzc_K540M with Y713E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc_K540M with Y713E

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

K540M, Y713E

Promoter

araBAD

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pKP1193

Plasmid

#167614

Purpose

Express TonB H20N

Depositor

Kathleen Postle

Article

Swayne et al J Bacteriol. 2011 Aug;193(15):3693-701.

Insert

tonB H20N

Expression

Bacterial

Mutation

tonB with H20N mutation

Available Since

July 9, 2021

Availability

Academic Institutions and Nonprofits only

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C511-E15: attB5r-ffluc2-nostop-attB1r

Plasmid

#162939

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal Nanoluciferase fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Firefly luciferase

Use

Synthetic Biology

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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C453-E04: attB4-CMV51p>-attB5

Plasmid

#162973

Purpose

Gateway attB4/attB5 entry clone for mammalian expression using an optimized human CMV promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

optimized human CMV promoter

Use

Synthetic Biology

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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C415-E01: attB4-stuffer-attB1r

Plasmid

#162931

Purpose

Gateway attB4-attB1r entry clone containing a non-transcribed/translated region for use in generating negative control vectors

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

stuffer fragment for vector controls

Use

Synthetic Biology

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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