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BOLL_RRM_pGEX

Plasmid

#135130

Purpose

Recombinant expression of BOLL with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

BOLL (BOLL Human)

Tags

GST-SBP

Expression

Bacterial

Promoter

tac

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pTE4561

Plasmid

#107525

Purpose

Expresses Lb crRNA and mCherry in mammalian cells.

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

Inserts

Lb crRNA
mCherry

Expression

Mammalian

Promoter

CBh and human U6

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pHA-N1-GalT

Plasmid

#87326

Purpose

To express GalT-HA in mammalian cells, GalT encodes the 1-81 amino acids from β1,4-galactosyltransferase

Depositor

Lei Lu

Article

Tie et al Mol Biol Cell. 2016 Jan 13. pii: mbc.E15

Insert

1-81 amino acids from β1,4-galactosyltransferase (B4GALT1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV-F

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pCAGGS_m-AKAR5’

Plasmid

#114140

Purpose

Mammalian expression of the AKAR5’ sensor C-terminally fused to the C-terminus of KRas (membrane targeting) for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM

Depositor

Haining Zhong

Article

Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

Insert

AKAR5' C-terminally tagged by KRas C tail

Expression

Mammalian

Available Since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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L2E-2

Plasmid

#21194

Depositor

Paul Khavari

Article

Scholl et al Cancer Res. 2004 Sep 1;64(17):6035-40

Insert

Mek2 (Esr1, MAP2K2 Mouse, Human)

Use

Retroviral

Mutation

deletion of residues EQQKKRLE, S222D S226D in Me…

Available Since

July 24, 2009

Availability

Academic Institutions and Nonprofits only
pX330-Utf1 gRNA

Plasmid

#128842

Purpose

gRNA for targeting mouse Utf1 locus using CRISPR-cas technique

Depositor

Yossi Buganim

Article

Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

Insert

Utf1 gRNA (Utf1 Mouse)

Use

CRISPR

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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HOXD4 (murine) HIS-tag pET

Plasmid

#8548

Depositor

Corey Largman

Article

Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

Insert

HOXD4 (Hoxd4 Mouse)

Tags

His and T7

Expression

Bacterial

Mutation

makes protein by Western and by in vitro transcri…

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
His-MBP-NRBF2 (1-159)

Plasmid

#99331

Purpose

Mammalian expression of the monomeric form of NRBF2 that binds with the PI3K C1 complex

Depositor

James Hurley

Article

Young et al Proc Natl Acad Sci U S A. 2016 Jul 19;11

Insert

Nuclear receptor-binding factor 2 (NRBF2 Human)

Tags

6xHis-MBP-TEV

Expression

Bacterial

Mutation

AA1-159 are present

Promoter

T7

Available Since

Aug. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX Grb7-PIR-SH2

Plasmid

#46443

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Grb7 (GRB7 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 334-532

Promoter

Tac

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pAGM_Puro_shRNA n.2_mTet3

Plasmid

#79625

Purpose

Expression of shRNA for mouse Tet3

Depositor

Silvia Monticelli

Article

Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

Insert

Tet3 (Tet3 Mouse)

Use

Lentiviral

Tags

none

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only
pAGM_Puro_shRNA n.1_mTet3

Plasmid

#79624

Purpose

Expression of shRNA for mouse Tet3

Depositor

Silvia Monticelli

Article

Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

Insert

Tet3 (Tet3 Mouse)

Use

Lentiviral

Tags

none

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only
gh142

Plasmid

#106812

Purpose

expression of gRNA targeting EOGT

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

EOGT (EOGT Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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gh6

Plasmid

#106690

Purpose

expression of gRNA targeting GALNT6

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

GALNT6 (GALNT6 Human)

Expression

Mammalian

Available Since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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pEF Doc2beta 6X mutant GFP

Plasmid

#128823

Purpose

To visualize Doc2beta 6X mutant in PC12 cells

Depositor

Edwin Chapman

Article

Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

Insert

Doc2beta (Doc2b Rat)

Tags

EGFP

Expression

Mammalian

Mutation

D163A, D218A, D220N, D303A, D357A, D359A

Promoter

EF-1alpha

Available Since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX HSH2-SH2

Plasmid

#46446

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

HSH2 (HSH2D Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 1-134

Promoter

Tac

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pEMS2058

Plasmid

#49136

Purpose

AAV plasmid with CAGGS promoter driving expression of EmGFP. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-CAGGS-emGFP-WPRE

Use

AAV

Expression

Mammalian

Promoter

CAGGS

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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gh5

Plasmid

#106689

Purpose

expression of gRNA targeting GALNT5

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

GALNT5 (GALNT5 Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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gh14

Plasmid

#106698

Purpose

expression of gRNA targeting GALNT14

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

GALNT14 (GALNT14 Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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gh13

Plasmid

#106694

Purpose

expression of gRNA targeting GALNT13

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

GALNT13 (GALNT13 Human)

Expression

Mammalian

Available Since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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pBT3102

Plasmid

#122548

Purpose

Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a plasma membrane localized gene (aqp-1) under the intestinal specific promoter nhx-2

Depositor

Gary Silverman

Article

Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

Insert

Pnhx-2_aqp-1::CemOrange2 (aqp-1 Synthetic, Nematode)

Expression

Bacterial

Promoter

Pnhx-2

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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